Table 3: Morphology of cysts of intestinal Amoebae

Question 1

Entamoeba histolytica

Size
Shape
No. of nucleus

Answer 1

• 10-20um:usual range, 12-15um
• usually spherical
• 4 if mature; 1or2 if immature

Question 2

Entamoeba hartmanni

Size
Shape
No. of nucleus

Answer 2

• 5-10um: usual range, 6-8um
• Usually spherical
• Similar to E. histolytica

Question 3

Entamoeba coli

Size
Shape
No. of nucleus

Answer 3

• 10-35um: usual range, 15 -25um
• Usually spherical: sometimes oval, triangular, or of shape
• 8 if mature; 16 if super nucleate cyst

Question 4

Endolimax nana

Size
Shape
No. of nucleus

Answer 4

• 5-10um: usual range, 6-8um
• Spherical, ovoid, or ellipsoidal
• 4 if mature; less than 4 if immature

Question 5

lodamoeba buetschlii

Size
Shape
No. of nucleus

Answer 5

• 5-20um:usual range,10 -12um
• ovoid ellipsoidal, triangular, or of
  another shape
• 1 if mature

Question 6

Entamoeba histolytica

Peripheral Chromatin
Karyosomal chromatin

Answer 6

• present: fine, uniform granules, evenly distributed
• Small, discrete, usually centrally located

Question 7

Entamoeba hartmanni

Peripheral Chromatin
Karyosomal chromatin

Answer 7

• present: fine, uniform granules, evenly distributed
• Small, discrete, usually centrally located

Question 8

Entamoeba coli

Peripheral Chromatin
Karyosomal chromatin

Answer 8

• present:coarse granules irregular
  in size and distribution,
• Large, discrete, usually eccentrically ,but occasionally centrally, located

Question 9

Endolimax nana

Peripheral Chromatin
Karyosomal chromatin

Answer 9

• none
• Large (blotlike), usually centrally located

Question 10

lodamoeba buetschlii

Peripheral Chromatin
Karyosomal chromatin

Answer 10

• none
• Large, usually eccentrically located; refractile, achromatic granules on Granules one side of karyosome; indistinct in iodine preparations present

Question 11

Entamoeba histolytica cytoplasm

Chromatoid bodies
Glycogen and other features

Answer 11

• Present: elongated bars with bluntly rounded ends
• Usually diffuse: concentrated mass often in young cysts; stains reddish brown with iodine

Question 12

Entamoeba hartmanni cytoplasm

Chromatoid bodies
Glycogen and other features

Answer 12

• Present: elongated bars with bluntly rounded ends; may be rounded and grapelike
• Similar to E. hisolytica

Question 13

Entamoeba coli cytoplasm

Chromatoid bodies
Glycogen and other features

Answer 13

• Present, but less frequently seen Than in E. histolytica; usually splinterlike with pointed ends
  -Usually diffuse, but occasionally well-defined mass in immature cysts; stains
  reddish brown with iodine

Question 14

Endolimax nana cytoplasm

Chromatoid bodies
Glycogen and other features

Answer 14

• Granules occasionally or small oval
  masses present, but bodies as seen in Entamoeba species are not present
• Usually diffuse; concentrated mass occasionally in young cysts; stains reddish brown with iodine

Question 15

Iodamoeba buetschlii cytoplasm

Chromatoid bodies
Glycogen and other features

Answer 15

• occasionally present, but bodies as seen in Entamoeba species are not present
• Compact, welldefined mass; stains
  dark brown with iodine