TCA Cycle

Question 1

What activates pyruvate dehydrogenase complex?

Answer 1

The substrates activate by inhibiting the kinase: ADP and pyruvate

Question 2

What inhibits pyruvate dehydrogenase complex?

Answer 2

The products inhibit by activating the kinase: AcCoA and NADH

Question 3

What cofactors are needed for the PDHC?

Answer 3

1. ThiaminePP=E1 decarboxylate
2. Lipoic Acid=E2 oxidizes
3. FAD=E3 rexodizes lipoic acid
4. NAD
5. CoA

Question 4

What are the 4 irreversible enzymes in the TCA cycle?

Answer 4

1. pyruvate dehydrogenase
2. citrate synthase
3. isocitrate DH
4. alphaKG DH

Question 5

How much ATP does 1 AcCoA produce?

Answer 5

12 ATP

Question 6

Which enzymes produce NADH?

Answer 6

1. Isocitrate DH
2. aplhaKG DH
3. Malate DH

Question 7

How many carbon dioxides are produced per 1 AcCoA?

Answer 7

2 CO2

Question 8

What is the rate limiting step in TCA?

Answer 8

Isocitrate dehydrogenase

Question 9

What inhibits PDH?

Answer 9

ATP, AcCoA, and NADH

Question 10

What inhibits citrate synthase?

Answer 10

ATP and citrate

Question 11

What inhibits isocitrate dehydrogenase?

Answer 11

ATP and NADH

Question 12

What activates isocitrate dehydrogenase?

Answer 12

ADP

Question 13

What inhibits alphaKGDH?

Answer 13

Succinyl-CoA, NADH, and ATP

Question 14

What enzyme is actually located in the mitochondrial membrane and used in ETC?

Answer 14

succinate dehydrogenase