Tecniques Of Genetic Analysis Flashcards Preview

Biochemestry and Medical Genetics > Tecniques Of Genetic Analysis > Flashcards

Flashcards in Tecniques Of Genetic Analysis Deck (5)
Loading flashcards...
1
Q

What other situation is mandatory to ask PCR to detect HIV infection rather than ELISA or WB?

A

Early testing after known exposure to HIV-positive blood (ex; needle sticks) or other fluids/tissue

2
Q

What test is used to study infants whose mothers are HIV positive? In what time of age?

A

PCR (test for the integrated proviral genome, HIV DNA PCR)
- advantages:
✔️(+) much earlier after infection
✔️Doesn’t rely on an Ab response
*useful because antibody test is uninformative (ELISA and WB)
*from birth until 18 month

2
Q

What test is use to measure the concentration of viral load in AIDS patient?

A

Reverse transcriptase PCR (HIV RNA Assay)

3
Q

What is and uses of a short tandem repeats (STR)?

A

Repeats of di- to tetra nucleotide sequence (spacer, between and within gene regions)
- uses: as known and documented in chromosome maps, use to genetic testing (Test for CAG repeat in Huntington disease by PCR)

4
Q

What is a RFLP and its utility?

A
  • Mutations in restriction endonuclease sites (DNA restriction fragments)
  • Southern blot
  • infer presence of a disease-causing allele of a gene in a family. Ex; sickle cell anemia - its mutation destroys a restriction site endonuclease.