Term 1-L8- Intro to Histo Flashcards
(31 cards)
Resolution
Minimum distance by which 2 points can be distinguished (ability to focus)
Low power microscopy is used to distinguish
Tissue organization
Intermediate power microscopy is used to distinguish
Cell organization
High power microscopy is used to distinuish
Cell/nuclear detail
Light microscopy (LM)
Photons of light interact with tissue components. Focused with optical lense.
Electron Microscopy (EM). Two types
Electrons interact with tissue components. Focused with elctromagnetic lens. TEM and SEM
Transmission Electron Microscopy (TEM)
Shows INTERNAL structure. Can be used to split membranes by TEM freeze fracture technique.
What kind of structure are dark in a TEM photo
Electron dense structures
What kind of structure are light in a TEM photo
Electron lucent structures
Base-like stains interact with
acids (basophilic structures)
Scanning Electron Microscopy (SEM)
This shows the EXTERNAL structure (surface) of a molecule
What color would structures like nucleic acids or glycosaminoglycans be stained
Blue or Purple
Acid-like stains interact with
bases aka acidophilic structures
What color would mitochondria and proteins like collagen be stained
pink, red, or orange
A base-like stain
Hematoxylin
An acid-like stain
Eosin
Periodic-acid Schiff (PAS) stains what color
Magenta
Periodic-acid Schiff (PAS) stains what structures
Carbohydrates ie. glycoproteins
Trichome stains what color
Blue or green
Trichrome stains what structures
Collagen
Silver stains what color
Black
Silver stains what structures
Reticular fibers
Orcein stains what color
Reddish-brown or black
Orcein stains what structure
Elastic fibers
