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H/E: Term 1- Exam 2 > Term 1-L8- Intro to Histo > Flashcards

Term 1-L8- Intro to Histo Flashcards

1
Q

Resolution

A

Minimum distance by which 2 points can be distinguished (ability to focus)

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2
Q

Low power microscopy is used to distinguish

A

Tissue organization

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3
Q

Intermediate power microscopy is used to distinguish

A

Cell organization

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4
Q

High power microscopy is used to distinuish

A

Cell/nuclear detail

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5
Q

Light microscopy (LM)

A

Photons of light interact with tissue components. Focused with optical lense.

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6
Q

Electron Microscopy (EM). Two types

A

Electrons interact with tissue components. Focused with elctromagnetic lens. TEM and SEM

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7
Q

Transmission Electron Microscopy (TEM)

A

Shows INTERNAL structure. Can be used to split membranes by TEM freeze fracture technique.

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8
Q

What kind of structure are dark in a TEM photo

A

Electron dense structures

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9
Q

What kind of structure are light in a TEM photo

A

Electron lucent structures

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10
Q

Base-like stains interact with

A

acids (basophilic structures)

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11
Q

Scanning Electron Microscopy (SEM)

A

This shows the EXTERNAL structure (surface) of a molecule

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12
Q

What color would structures like nucleic acids or glycosaminoglycans be stained

A

Blue or Purple

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13
Q

Acid-like stains interact with

A

bases aka acidophilic structures

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14
Q

What color would mitochondria and proteins like collagen be stained

A

pink, red, or orange

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15
Q

A base-like stain

A

Hematoxylin

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16
Q

An acid-like stain

A

Eosin

17
Q

Periodic-acid Schiff (PAS) stains what color

A

Magenta

18
Q

Periodic-acid Schiff (PAS) stains what structures

A

Carbohydrates ie. glycoproteins

19
Q

Trichome stains what color

A

Blue or green

20
Q

Trichrome stains what structures

A

Collagen

21
Q

Silver stains what color

A

Black

22
Q

Silver stains what structures

A

Reticular fibers

23
Q

Orcein stains what color

A

Reddish-brown or black

24
Q

Orcein stains what structure

A

Elastic fibers

25
Q

Sudan red stains what color

A

Red

26
Q

Saudan red stains what structures

A

Lipids

27
Q

Osmic acid stains what structures

A

Lipids

28
Q

Osmic acid stains what color

A

Black

29
Q

Lower power magnification is used to visualize

A

Tissue organization

30
Q

Intermediate power magnification is used to viualize

A

Cell organization

31
Q

High power magnification is used to visualize

A

Cell/nuclei detail

H/E: Term 1- Exam 2 (1 decks)

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