test 2 (chapter 5,6,8,) Flashcards
(100 cards)
What is a gene composed of
DNA that transcribes to produce an RNA molecule during transcription.
what is mRNA and what does it do
Messenger RNA and it provides the information for the ribosome to catalyze protein synthesis in translation.
what is gene expression?
the synthesis of a specific protein with a sequence of amino acids that is encoded into a gene.
What is central dogma
the flow of genetic information from DNA to RNA to protein.
what is a cells genotype
its full collection of its genes it contains.
What is a cells phenotype
The set of observable characteristics from those genes.
semiconservative replication
after replication each parent strand has a new and an old strand
What is DNA pol 3
is required from DNA synthesis and adds deoxyribose nucleotides one by one on the 3’ -OH strand.
what are DNA pol 1 and 2 for
repair.
what is a histone
a protein that provides structural support for a chromosome.
What happens if DNA is supercoiled?
it is inaccessible.
How does DNA get un-supercoiled?
Topoisomerase(gyrase)
What does helicase do
break the DNA structure by destroying the hydrogen bonds.
What nucleotide has fewer hydrogen bonds?
AT
What is primer?
a free 3’ -OH group that start the DNA synthesis.
what is primer synthesized by
RNA primase.
Ligase
Seals the gaps between the Okazaki fragments on the lagging strand to create one continuous DNA strand
DNA pol 1
Exonuclease activity removes RNA primer and replaces it with newly synthesized DNA
Topoisomerase II (DNA gyrase)
Relaxes supercoiled chromosome to make DNA more accessible for the initiation of replication; helps relieve the stress on DNA when unwinding, by causing breaks and then resealing the DNA
Topoisomerase IV
Introduces single-stranded break into concatenated chromosomes to release them from each other, and then reseals the DNA
A segment of DNA that codes for a functional product
gene
What process converts one parental DNA strand into 2 identical offspring molecules
transcription
In DNA replication the newly added nucleotide is joined to the growing DNA strand by
DNA polymerase
DNA polymerase adds nucleotides specifically to the what prime side of the growing strand?
3 prime