Test 2 - practice test content Flashcards

(48 cards)

1
Q

what kind of alpha linkage is glycogen made up of?

A

alpha 1,4 (strain chains) and 16, linkages (branch points)

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2
Q

What protein transports fatty acids in the blood?

A

albumin

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3
Q

What is the main protein in blood plasma

A

albumin

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4
Q

where is albumin made

A

in the liver

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5
Q

name all that albumin transports

A

fatty acids, hormones, drugs, bilirubin (residue of broken down red blood cells)

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6
Q

what is albumin’s function other than transport

A

maintains blood pressure by keeping water in the blood vessels

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7
Q

how does albumin carry fatty acids

A

albumin is a hydrophobic pocket that binds to fatty acids (also hydrophobic)

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8
Q

Why do some hormones need carriers and others dont?

A

Steroid hormones (like estrogen, testosterone) need carriers because they’re fat-soluble.
Peptide hormones (like insulin, glucagon) don’t — they just float in the blood.

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9
Q

what is acetylcholine

A

Acetylcholine (ACh) is a neurotransmitter — a chemical that nerve cells use to send signals.

It’s involved in:

Muscle contraction

Memory and learning

Parasympathetic nervous system (“rest and digest”)

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10
Q

What is rhodopsin

A

Rhodopsin is a light-sensitive protein found in the rods of the retina (in your eye)
It’s essential for night vision (helps you see in low light)
membrane-bound protein (in eye)
made of a protein called opsin + retinal

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11
Q

what is adrenaline/epinephrine

A

Adrenaline (also called epinephrine) is a hormone and neurotransmitter

Made by your adrenal glands (above the kidneys)

Released during stress or danger — part of the “fight or flight” response => Increases heart rate, Raises blood pressure, Boosts blood glucose, Opens airways, Prepares muscles for action

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12
Q

how is adrenaline carried in the body

A

Adrenaline is water-soluble, so it dissolves directly in the blood

No carrier protein is needed

It travels through the bloodstream to reach target organs quickly

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13
Q

In glycolysis, ATP molecules are produced by ____

A

substrate-level phosphorylation

It means a phosphate group is directly transferred from a high-energy molecule (like 1,3-BPG or PEP) to ADP to make ATP

No oxygen or mitochondria are needed — happens right in the cytoplasm

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14
Q

Whats the purpose of glycolysis

A

Breakdown of glucose to make energy

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15
Q

In glycolysis, glucose is broken down into what first? give the enzyme and reactants/products

A

glucose => glucose-6-phosphate. hexokinase is the enzyme that takes a phosphate from ATP and transforms it into ADP to give to glucose.
hexo = 6C
kinase = phosphorylation

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16
Q

in glycolysis, glucose-6-phosphate is transformed into what? give the enzyme and reactants/products

A

glucose-6-phosphate => fructose-6-phosphate.
isomerase is used. its just a rearrangement

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17
Q

in glycolysis, fructose-6-phosphate is transformed into what? give the enzyme and reactants/products

A

fructose-6-phosphate => fructose-1,6-biphosphate.
addition of a phosphate at 1
by phospho-fructokinase
1 ATP becomes ADP

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18
Q

in glycolysis, fructose-1,6-phosphate is transformed into what? give the enzyme and reactants/products

A

glyceraldehyde-3-phosphate and dihydroxyacetone phosphate (isomers).
done by aldolase

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19
Q

in the second phase of glycolysis, glyceraldeyde-3-phosphate is transformed into what? give the enzyme and reactants/products

A

glyceraldeyde-3-phosphate => 1-3bisphospho-glycerate.
Done by Gl-3-P, PO4 and dehydrogenase (the H is given to NAD => NADH)

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20
Q

in the second phase of glycolysis, 1-3bisphospho-glycerate is transformed into what? give the enzyme and reactants/products

A

1-3bisphospho-glycerate. => 3-phospho-glycerate
Done by phosphoglycerate kinase
ADP => ATP

21
Q

in the second phase of glycolysis, 3-phospho-glycerate is transformed into what? give the enzyme and reactants/products

A

3-phospho-glycerate => 2-phospho-glycerate.
done by phosphoglycerate mutase

22
Q

in the second phase of glycolysis, how is 2-phospho-glycerate is transformed into pyruvate? give the enzyme and reactants/products

A

2-phospho-glycerate => phosphoenol pyruvate => pyruvate
done by enolase MG2+ and pyruvate kinase
ADP => ATP

23
Q

give the net reaction of glycolysis

24
Q

The 4 different redox centers found in protein complexes I–IV of the Electron Transport Chain (ETC) are….

A

Flavin mononucleotide (FMN)/coenzymes

Iron-sulfur (Fe-S) clusters

Cytochromes (with heme groups)

Copper (Cu) centers

25
what is the role of activated protein kinase in the signaling cascade?
phosphorylates proteins
26
What converts ATP to cAMP?
Adenylyl cyclase (AC) in inner side of plasma membrane/inside the cell When certain hormones or signals bind to G-protein-coupled receptors (GPCRs), they activate a G protein (Gαs) That G protein activates adenylyl cyclase Adenylyl cyclase converts ATP → cyclic AMP (cAMP) by forming a ring structure
27
what is cAMP and why does it matter
cAMP is a second messenger It activates protein kinase A (PKA) That leads to lots of downstream effects, like: Breaking down glycogen Increasing heart rate Regulating gene expression
28
What degrades cAMP to AMP?
Phosphodiesterase (PDE) Phosphodiesterase breaks the cyclic bond in cAMP This converts cAMP → AMP, effectively turning off the signal Keeps the cAMP signal short-lived Helps the cell reset and avoid overstimulation
29
What activates adenylate cyclase? how do we get from that to cAMP?
The Gαs subunit of a G-protein, after activation by a G-protein-coupled receptor (GPCR). A ligand (like epinephrine or glucagon) binds to a GPCR on the cell surface This activates a G-protein inside the membrane The Gαs subunit swaps GDP for GTP (gets "turned on") Gαs then activates adenylate cyclase Adenylate cyclase converts ATP → cAMP
30
what reducing equivalent is used in gluconeogenesis?
NADH
31
what releases insulin
beta cells in pancreas
32
how many atp and gtp does gluconeogenesis need
4 atp and 2 gtp
33
what is gluconeogenesis
synthesis of glucose from a non carb source like fat, amino acids, pyruvate
34
where is glycogen stored
in the liver
35
what does glucagon do when blood glucose levels fall
glucagon cause the liver to break down glycogen
36
what is glucagon
Glucagon is a hormone made by the alpha cells of the pancreas. Glucagon binds to glucagon receptors on liver cells Activates adenylate cyclase → cAMP → PKA pathway This triggers: Glycogen breakdown (glycogenolysis) Glucose production from amino acids (gluconeogenesis) Inhibition of glycolysis in the liver
37
how many protons each complex pumps out in the electron transport chain (ETC)
38
Does calcium inhibit the TCA cycle?
No — calcium actually activates the TCA cycle. It stimulates key enzymes in the TCA cycle to boost energy (ATP) production, especially in active tissues like muscle. It activates: Pyruvate dehydrogenase (PDH) Isocitrate dehydrogenase Alpha-ketoglutarate dehydrogenase
39
Is PDH regulated by feedback inhibition from pyruvate and NADH?
no only nadh not pyruvate (it activates it)
40
what are some inhibitors of PDH
NADH – signals high energy, so PDH slows down Acetyl-CoA – product of PDH, so it feeds back to slow it down ATP – also signals high energy, and indirectly inhibits PDH
41
what are some activators of pdh
Pyruvate – doesn’t inhibit, it helps activate PDH by inhibiting PDH kinase (which normally inactivates PDH) Calcium (Ca²⁺) – activates PDH in muscle cells to make more energy ADP – signals low energy, promotes PDH activity
42
whats the role of pdh
PDH = Pyruvate Dehydrogenase It’s an enzyme complex that converts: Pyruvate → Acetyl-CoA
43
name three things that can be used for metabolic control mechanism for glycogen phosphorylation
action of hormone, covalent modification of an enzyme, allosteric regulation (Allosteric regulation is when a molecule binds to an enzyme at a site other than the active site (called the allosteric site) to change the enzyme's activity.)
44
what is acetyl-coa converted to before it can leave the mitochondrial matrix
citrate intermediate
45
give the products of the citric acid cycle
co2, fadh2, atp, nadh
46
how many nadh are produced in glycolysis
2
47
name the four coenzymes that participate in pyruvate dehydrogenase reactions
thiamine pyrophosphate, fad, nad+, coA
48
the pentose pathway produces what
nadph