TEst 3-9 (Major groups disease causing bacteria)

Question 1

Gram-positive bacteria

Answer 1

• Aerobic Gram-positive Rods
• Anaerobic Spore-Forming Gram-positive Rods
• Gram-positive cocci

Question 2

Aerobic Gram-positive Rods

Answer 2

o Bacillus
o Corynebacterium
o Listeria

Question 3

Anaerobic Spore-Forming Gram-positive Rods

Answer 3

o Clostridium

Question 4

Gram-positive cocci

Answer 4

o Staphylococcus
o Streptococcus
o Enterococcus

Question 5

Gram-negative bacteria

Answer 5

• Gram-negative Cocci
• Enteric Gram-negative rods
• Nonenteric Gram-negative rods

Question 6

Gram-negative Cocci

Answer 6

o Neisseria

Question 7

Enteric Gram-negative rods

Answer 7

o Escherichia
o Campylobacter
o Yersinia enterocolitica
o Salmonella
o Shigella
o Helicobacter
o Vibrio
o Bacteroides

Question 8

Nonenteric Gram-negative rods

Answer 8

o Haemophilus
o Legionella
o Francisella
o Pasteurella
o Brucella
o Rickettsia
o Pseudomonas
o Klebsiella
o Yersinia pestis

Question 9

Bacteria not usually Gram-stained

Answer 9

• Elementary body/Reticulate body
• Spirochetes
• No-cell wall
• Unique cell wall

Question 10

Elementary body/Reticulate body

Answer 10

o Chlamydia

o Chlamydophila

Question 11

Spirochetes

Answer 11

o Borrelia

o Treponema

Question 12

No-cell wall

Answer 12

o Mycoplasma

Question 13

Unique cell wall

Answer 13

o Mycobacterium

Question 14

Bacillus anthracis morpology

Answer 14

• Aerobic Gram-positive Rods
• anthrax- biological warfare/bioterrorism agent
• morphology: large end to end chains
• Produces spores
• No motility
• Poly-D-glutamic acid capsule; spores

Question 15

Bacillus cereus morpology

Answer 15

• Aerobic Gram-positive Rods
• food poisoning
• morphology: large
• Produces spores
• 50% of strains have motility
• Spores

Question 16

Corynebacterium diphtheriae morpology

Answer 16

• Aerobic Gram-positive Rods
• diphtheria
• morpholoy: Small, narrow
• No spores
• No motility
• Pleomorphic; Chinese characters (snapping fission)

Question 17

Listeria monocytogenes morpology

Answer 17

• Aerobic Gram-positive Rods
• meningtis - foodborne
• morpholoy: small
• No spores
• tumbling motility
• Grows at 3-4oC; grows intracellularly

Question 18

Streptococcus - -catalase rxn and morphology

Answer 18

-catalase negative
-grow in chains
Gram-Positive Cocci

Question 19

Staphylococcus -catalase rxn and morphology

Answer 19

-catalase positive
-grow in large clusters
Gram-Positive Cocci

Question 20

Streptococcus pyogenes-catalase/bacaitracin/optochin rxn and morphology

Answer 20

• Gram-positive cocci
• hemolysis beta
• sensitive to bacitracin

Question 21

Streptococcus agalactiae–catalase/bacaitracin/optochin rxn and morphology

Answer 21

• Gram-positive cocci
• hemolysis beta usually, sometimes gamma
• resistant to bacitracin

Question 22

Streptococcus pneumoniae-catalase/bacaitracin/optochin rxn and morphology

Answer 22

• Gram-positive cocci
• hemolysis alpha; Viridans group
• -resistant to bacitracin
• sensitive to optochin
• looks green around colony
• clinical specimens appear lancet shaped or bullet shaped diplococcus

Question 23

Staphylococcus aureus - coagulase and novobiocin rxn

Answer 23

• coagulase positive

- novobiocin sensitive

Question 24

Staphylococcus epidermidis - coagulase and novobiocin rxn

Answer 24

• coagulase negative

- novobiocin sensitive

Question 25

Staphylococcus saprophyticus - coagulase and novobiocin rxn

Answer 25

- coagulase negative | - novobiocin resistant

Question 26

Clostridium botulinum - morphology, spore formation and motility?

Answer 26

Anaerobic Spore-Forming Gram-Positive Rods | MOTILE

Question 27

Clostridium tetani-- morphology, spore formation and motility?

Answer 27

Anaerobic Spore-Forming Gram-Positive Rods | MOTILE

Question 28

Clostridium perfringens-- morphology, spore formation and motility?

Answer 28

Anaerobic Spore-Forming Gram-Positive Rods | ONLY CLOSTRIDIUM THAT IS NOT MOTILE

Question 29

Neisseria gonorrhoeae -maltose fermentation test

Answer 29

- Gram-Negative Cocci - kidney bean shaped (diploccocus) - Maltose NEGATIVE (Can ONLY use glucose)

Question 30

Neisseria meningitidis-maltose fermentation test

Answer 30

- Gram-Negative Cocci - kidney bean shaped (diploccocus) - Maltose POSITIVE (it can use maltose and glucose)

Question 31

Escherichia - lactose fermentation and H2S production tests

Answer 31

- Gram-Negative Rods - YES Lactose Fermention - NO H2S gas

Question 32

Salmonella- lactose fermentation and H2S production tests

Answer 32

- Gram-Negative Rods - NO Lactose Fermention - YES H2S gas

Question 33

Shigella- lactose fermentation and H2S production tests

Answer 33

- Gram-Negative Rods - NO Lactose Fermention - NO H2S gas

Question 34

Yersinia- lactose fermentation and H2S production tests

Answer 34

- Gram-Negative Rods - NO Lactose Fermention - NO H2S gas

Question 35

Chlamydia and Chlamydophila (cell wall/acid fast staining/wrights stain/obligate intra-pathogens)

Answer 35

- Bacteria Not Usually Gram Stained | - obligate intra cellular pathogen

Question 36

Borrelia (cell wall/acid fast staining/wrights stain/obligate intra-pathogens)

Answer 36

- Bacteria Not Usually Gram Stained | - Identify with Wright stain

Question 37

Treponema (cell wall/acid fast staining/wrights stain/obligate intra-pathogens)

Answer 37

-Bacteria Not Usually Gram Stained | ??

Question 38

Mycobacterium (cell wall/acid fast staining/wrights stain/obligate intra-pathogens)

Answer 38

- Bacteria Not Usually Gram Stained | - acid fast staining

Question 39

Mycoplasma (cell wall/acid fast staining/wrights stain/obligate intra-pathogens)

Answer 39

- Bacteria Not Usually Gram Stained | - NO CELL WALL