test 6 Flashcards
(15 cards)
DNA methylation
the addition of methyl groups to dna (often at cytosine bases) which typically suppresses gene expression by preventing transrciption factors from binding.
Histone acetylation
the addition of histone to acetyl proteins loosening the dna wrapped around them and allowing genes to be more easily transcribed
Enhancers
Dna sequences that increase the rate of transcription when specific proteins (activators) bind to them, even if they are far from the gene
Repressor Proteins
Proteins that bind to specific DNA sequences (like silencers or operators) and block transcriptionn by preventing RNA polymerase from binding
Transcription Factors
Proteins that help regulate gene expression by binding to specifc DNA sequences and assisiting ot blocking RNA polymerase
Master genes
Genes that control the expression of many other genes and direct large developmental processes like body plan formation
RNAi
A process where small RNA molecules (like siRNA or miRNA) bind to mRNA and either block translation or cause the mRNA to be destrpyed reducing gene expression.
5’ Cap
A modified guanine nucleotide added to 5’ end of the mRNA for protection and ribosome binding
Alternative Splicing
A process during mRNA editing where different exons are combined various ways to produce multiple proteins from one gene
Cell Differentiation
The process where unspecialized cells (like stem cells) become specialized in structure and function through gene regulation
Universal Genetic Code
the nearly identical way that codons (triplets of RNA bases) specify amino acids in all living organisms - evidence of a common ancestor
Stickleback Gene regulation
In stickleback fish, chnages in gene regulation (like turning off pelvic genes via switches) show how evolution can act on DNA control elements not just genes themselves
Gel Electrophoresis
A technique to separatte DNA fragments by size. DNA is loaded into gel and pulled throuogh by an electric current; smaller fragments move faster
Restriction Enzymes
Proteins that cut DNA at a specific sequences often used to prepare DNA for gel electrophores or to clone genes
PCR ( Polymerase Chain Reaction )
A method to amplify (make many copies of) specific DNA segments using heat cycles and enzymes, often used before gle electrophoresis