test 6 Flashcards

(15 cards)

1
Q

DNA methylation

A

the addition of methyl groups to dna (often at cytosine bases) which typically suppresses gene expression by preventing transrciption factors from binding.

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2
Q

Histone acetylation

A

the addition of histone to acetyl proteins loosening the dna wrapped around them and allowing genes to be more easily transcribed

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3
Q

Enhancers

A

Dna sequences that increase the rate of transcription when specific proteins (activators) bind to them, even if they are far from the gene

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4
Q

Repressor Proteins

A

Proteins that bind to specific DNA sequences (like silencers or operators) and block transcriptionn by preventing RNA polymerase from binding

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5
Q

Transcription Factors

A

Proteins that help regulate gene expression by binding to specifc DNA sequences and assisiting ot blocking RNA polymerase

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6
Q

Master genes

A

Genes that control the expression of many other genes and direct large developmental processes like body plan formation

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7
Q

RNAi

A

A process where small RNA molecules (like siRNA or miRNA) bind to mRNA and either block translation or cause the mRNA to be destrpyed reducing gene expression.

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8
Q

5’ Cap

A

A modified guanine nucleotide added to 5’ end of the mRNA for protection and ribosome binding

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9
Q

Alternative Splicing

A

A process during mRNA editing where different exons are combined various ways to produce multiple proteins from one gene

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10
Q

Cell Differentiation

A

The process where unspecialized cells (like stem cells) become specialized in structure and function through gene regulation

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11
Q

Universal Genetic Code

A

the nearly identical way that codons (triplets of RNA bases) specify amino acids in all living organisms - evidence of a common ancestor

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12
Q

Stickleback Gene regulation

A

In stickleback fish, chnages in gene regulation (like turning off pelvic genes via switches) show how evolution can act on DNA control elements not just genes themselves

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13
Q

Gel Electrophoresis

A

A technique to separatte DNA fragments by size. DNA is loaded into gel and pulled throuogh by an electric current; smaller fragments move faster

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14
Q

Restriction Enzymes

A

Proteins that cut DNA at a specific sequences often used to prepare DNA for gel electrophores or to clone genes

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15
Q

PCR ( Polymerase Chain Reaction )

A

A method to amplify (make many copies of) specific DNA segments using heat cycles and enzymes, often used before gle electrophoresis

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