Test 8 Lecure 3 Flashcards
How do you create recombinant DNA?
Fragment isolation using electrophoresis
A common technique to separate different fragments of cut DNA so that they can be isolated is ___________. This technique takes advantage of the negative charge on DNA by using an electrical field to separate DNA molecules based on size. Fill in the blank.
electrophoresis
Which statement does not describe the main steps of electrophoresis?
Take 3 restriction enzymes → Cutters depend on each enzymes recognition sequence
Load fragment into gel
Fragments move based on size
What can occur after finishing electrophoresis? (i.e. what is the final product and what can you do with it)
You can put fragments together to make recombinant DNA
Put together sticky ends (using DNA ligase)
DNA libraries are collections of recombinant DNA molecules that can be stably maintained and replicated , via ______________, in a suitable host organism. Fill in the blank.
According to the video PCR, what is the main function of PCR (polymerase chain reaction)?
According to the video PCR, why do we complete PCR (i.e. how can it be utilized)?
According to the video PCR, why do we have to use reverse transcriptase in order to complete PCR to diagnosis the disease COVID-19?
________ mimics the process of DNA replication and an individual reaction is cycled through a series of steps in which each step is analogous to a step in DNA replication. Fill in the blank.
PCR
During PCR, ___________ is when heat is used to separate strands of double-stranded DNA, _________n is when primers provide the 3’ OH required for elongation by DNA polymerase, and __________ is the last step and when the DNA polymerase makes new DNA. Fill in the blanks.
denaturation
annealing of primers
synthesis
Who is using DNA fingerprinting?
Mainly law enforcement
What is DNA fingerprinting?
Trying to find short tandem repeats (STRs) inside someone’s
DNA
STRs
PCR and STRs
Use PCR to get results very quickly and inexpensively
Which statement does not describe one of the three things we can now do involving gene editing?
- Change a gene’s sequences
- Inactivate a gene
- Interfere with the expression of the gene in the cell
Which statement describes one of the main ways we can edit genes in vivo (we discussed two in class)?
Either by using TALE proteins
T → transcription
A → activator
L → like
E → effector
Or CRISPR/Cas9
According to the video CRISPR Gene-Editing Reality Check, how does CRISPR/cas9 work? (know the steps discussed in the video)
