Test For nucleic Acids

Question 1

Test for base of nucleic acids

Answer 1

Purine and pyrimidine bases absorb maximally at 260nm (UV range) due to the aromatic rings.

Question 2

Test for sugar (ribose)

Answer 2

Orcinol test

Blue colour

Question 3

Test for sugar (deoxyribose) dddg

Answer 3

Diphenylamine/Dische test

Green colour

Question 4

Test for phosphate

Answer 4

Fiske subbarrow test/ammonium molybdate test

Red colour