Tests Flashcards

(43 cards)

0
Q

Purdy’s modification of Heat and HAc test

A

Sat. Soln. NaCl.
Glacial Acetic acid

Qualitative Protein
Coagulation or flocculation or precipitation of protein (turbidity to boiling solid)

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1
Q

Heat and HAc test

A

5% or 10% HAc

Qualitative Protein
Coagulation or flocculation or precipitation of protein (turbidity to boiling solid)

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2
Q

Picric acid test

A

sat. Picric acid

Qualitative Protein
Coagulation or flocculation or precipitation of protein (turbidity to boiling solid)

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3
Q

Osgood haskin’s test

A

50% HAc
Sat. Soln. NaCl.

Qualitative Protein
Coagulation or flocculation or precipitation of protein (turbidity to boiling solid)

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4
Q

Potassium Ferrocyanide Test

A

Glacial HAc
5% to 10% potassium
Ferrocyanide

Qualitative Protein
Coagulation or flocculation or precipitation of protein (turbidity to boiling solid)

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5
Q

Exton’s test ( or sulfosalicylic acid test)

A

Sulfosalicylic acid
Sodium sulfate

Turbidity or white rint

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6
Q

Spiegler’s test

A
30% HAc
Spiegler's reagent: (Sms)
- Succinic acid
- mercuric chloride
- sodium chloride

Turbidity or white ring

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7
Q

Heller’s test

A

Conc HNO3

White ring

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8
Q

Robert’s test

A

Robert’s reagent:

  • magnesium sulfate 7H2o
  • HNO3
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9
Q

Biuret reaction

A

10% NaOH or KOH
10% Cupric sulfate

Violet or rose color

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10
Q

Paper electrophoresis/ cellulose acetate electrophoresis

A

Refer to cc notes

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11
Q

Kwilecki’s modification if esbach’s test

A

10% FeCl
Esbach reagent:
- picric acid
- citric acid

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12
Q

Tsuchiya’s test

A

Tsuchiya’s reagent:

  • phosphotungstic acid
  • 95% alcohol
  • conc. HCL
  • barium sulfate or powdered pumicr
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13
Q

Purdy’s

A

50% HAc

10% potassium ferricyanide

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14
Q

Shanky and Stafford method

A

Tsuchiya’s reagent

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15
Q

Kingsbury and Clark Method

A

3% sulfosalicylic acid

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16
Q

Harrison’s test

A

HAc

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17
Q

Osgood-Haskin’s test

18
Q

Bradshaw test

19
Q

Heal precipitation

20
Q

Pulnam’s Method

A

2M acetate buffer

21
Q

Toluene sulfonic acid test

A

Toluene sulfonic acid

22
Q

Sulfosalicylic acid test

A

Sulfosal acid

23
Q

Jacobson and miner’s test

24
Protein paper extraction test
NaCl Acetate buffer Sulfosate acid
25
Method by hepler
Glacial Hac
26
Mucicarmine cytologic test
Mucicarmine stain
27
Benedict's test
Benedict's reagent: - copper sulfate - sodium citrate - sodium carbonate Green to yellow to orange red ppt
28
Fehling's test
Fehling's rgt: a = cupric sulfate B = rochelle salt, KOH
29
Haine's test
Haine's rgt
30
Trommer's test
10% KOH/NaOH | 10% Copper sulfate
31
Nylander's test
Nylander's rgt: - rochelle salt - 10% NaOH - bismuth subnitrate
32
Moore-heller's test
10% KOH
33
Yeast fermentation
Yeast
34
Phenylhydrazine test
Phenylhydrazine Hydrocholride sodium Acetate
35
Kowarsky's method
Phenylhydrazine Hydrochloride sodium Sat. NaCl
36
Benedict's quantitative test
Benedict's quantitative reagent: - copper sulfate - sodium carbonate - sodium citrate - potassium sulfocyanate - potassium ferrocyanate
37
Fehling's quantitative test
Fehling's quantitative reagent
38
Robert's fermentation test
Yeast Difference in Specific gravity
39
Einhorn's fermentation
Yeast Based on degree of fermentation
40
Millard-smith micromodification of benedict's
Benedict's reagent
41
Reagent strip test Glucose oxidase double Sequential enzyme reaction
Glucose oxidase Peroxidase Chromogen buffer
42
Oxygen rate method | Other tests for blood glucose
Cc notes