TESTS

Question 1

Gmelin

Answer 1

test for bile pigments

Question 2

Lugol’s iodine + alcoholic solution of zinc acetate

Answer 2

Schlesinger

Question 3

Schlesinger

Answer 3

test for bile pigments

Question 4

Schlesinger (+) result

Answer 4

greenish fluorescence

Question 5

Gmelin (+) result

Answer 5

play of colors

Question 6

tests for hemoglobin

Answer 6

Benzidine
Guaiac
Ortho-Toluidine

Question 7

(+) results for tests for hemoglobin

Answer 7

Benzidine = green-blue
Guaiac = blue
Ortho-Toluidine = blue

Question 8

Sulkowitch is a test for

Answer 8

Calcium

Question 9

(+) Sulkowitch test

Answer 9

precipitation

Question 10

benedict’s test (+)

Answer 10

green-orange-red

*Reducing substances

Question 11

test for lactose and glucose

Answer 11

Rubner’s test

Question 12

positive rubner’s test

Answer 12

Lactose: brick red with red ppt. (+)
glucose: red color with yellow precipitate

Question 13

Borchardt’s also known as

Answer 13

Seliwanoff’s

Question 14

Borchardt’s/Seliwanoff’s tests for

Answer 14

sugars/ fructose

Question 15

(+) Borchardt’s/Seliwanoff’s test

Answer 15

Red color

Question 16

lead acetate + ammonia H2O

Answer 16

Rubner’s

Question 17

benedict’s rgt. composition

Answer 17

CuSO4 +NaCO3 + Na citrate buffer

copper sulfate
sodium carbonate
sodium citrate buffer

Question 18

tests for Pentose

Answer 18

Bial orcinol

Tauber’s

Question 19

Fehling’s (+)

Answer 19

Yellow precipitate: (+) glucose

Question 20

Ictotest

Answer 20

for Bile pigments

• blue to purple mat (+)
• uses Ictotablets

Question 21

Schales and Schales also known as

Answer 21

Mercurimetric titration

*test for Chloride

Question 22

Rothera’s test reagents

Answer 22

sodium nitroprusside + ammonium sulfate

Question 23

Rothera’s test (+)

Answer 23

acetone and AAA = (+) Rose/purple ring

Question 24

KETONES

Answer 24

Acetoacetate
Acetone
Beta-Hydroxybutyric acid

Question 25

a test for ketones that uses Na nitroprusside

Answer 25

Legal

Question 26

Legal test (+)

Answer 26

``` Acetone = (+) purple/violet AAA = (+) Red ```

Question 27

a test for ketones that uses 10% FeCl3

Answer 27

Gerhardt's

Question 28

Gerhardt's (+)

Answer 28

AAA = (+) Bordeaux red color

Question 29

Qualitative test for protein that uses Exton's reagent (Na2SO4 + SSA + Dist. H2O)

Answer 29

Sulfosalicylic acid

Question 30

Biuret test is for

Answer 30

Qualitative test for protein

Question 31

Quantitative test for Protein

Answer 31

Esbach's Kwilecki's Tsuchiya's Kingsburry-Clark