The Microbial World and You Flashcards

Question

Discuss the other scienific observations that Robert Hooke had, including Cell theory.

Answer 1

Robert Hooke reported that living things were composed of little boxes, or cells, that in later years led to Cell Theory Cell Theory- that all living things are comprise of cells (Schwann's theory) -He also had his observations detailed in Micrographia, the first scientific best seller that contained illustrations drawn by Hooke himself (Drew fleas and gnats) -He was able to observe and see tiny honeycomb like structures of a cork which he referred to as cells (named after small rooms in monasteries) the cell structure of a cork (described as boxers) and drew fleas, gnats in his works.

Answer 2

Although Hooke's microscope showed LARGE cells, he lacked the resolution to see microbes clearly -the diameter of a Cork cell that he could see is 25 um (micrometers) -Hooke could not see bacteria that was 1 um

Answer 3

Scientist Anton Van Leewenhoek known as "The father of Microbiology" -from 1673-1723, Leewenhoek described live microorganisms in series of letters to the Royal Society of London he was a dutch merchant and amateur scientist -

Answer 4

Hooke's COMPOUND microscope increased magnification from 9x to 30x while van Leeuwenhoek's SIMPLE microscope magnified around 300 x

Answer 5

Because his lens was much better Leeuwenhoek saws protozoa, fungi and bacteria. He also made drawings of bacteria in 1683

Answer 6

Prior to the 1850s, many scientists and philosophers believed in Spontaneous Generation Spontaneous Generation: the hypothesis that living organisms could arise from nonliving matter; a vital force forms life They tried to prove it by observing maggots from decaying corpses and flies from manure -They found recipe for mice: Dry shirt + Wheat + 21 days = mice

Answer 7

experiment to test the spontaneous generation maggots: -1668: Francesco Redi filled jars with decaying meat (before van Leeuwenhoek) - wanted to pose question: Did maggots come from spontaneous generation or fly reproduction Results: No maggots appeared when flies prevented from laying their eggs -They had two jars and closed one jar with meat inside it, and no maggots were there -another jar was open with meat and maggots appeared to fly around - This experiment was criticized because you need AIR to test for spontaneous generation and closing the jar with meat in side prevented that

Answer 8

3 trials: 1. Flask sealed with meat inside jar: No maggots 2. Flask open with meat inside jar: maggots appear **New IDEA*** 3. Flask covered with GAUZE- saw no maggots, but flies were around; however flies were too complicated for spontaneous generation. Microbes could possibly undergo spontaneous generation. Conclusion: If large forms of life didn't arise form non life perhaps , MICROBES simple enough to be generating from Nonliving materials

Answer 9

Experiment FOR spontaneous generation: -in 1745, took a flask with broth and heated it up to boil, then opened the flask, let it sit and cool for some time and led to Microbial Growth Flaws: 1. when they cooled the flask, they OPENED the jar; things can float from the air into flask 2. Boiling does NOT kill endospores (bacteria producing endospores are resistant to boiling) Experiment AGAINST Spontaneous -in 1765, took a flask filled with broth an heat up to let boil, then SEALED the flask with a cap, and let sit and cool for some time; which led to NO GROWTH of microbes (hypthesize no spontaneous generation) Flaws: 1. The jar was blocked, so no air was available for microbes .

Answer 10

in 1858, the Biogenesis hypothesis further challenged the concept of Spontaneous Generation -Biogenesis hypothesis- stated that living organisms arise from preexisting life However, there was NO PROOF offered

Answer 11

Louis's Pasteur's experiment disproved spontaneous generation In1861: Louis PASTEUR designed a flask to let air in but seep out microbes -Pasteur has a special flask that had a swan neck so air could get in and microbes won't be able to reach inside flask.

Answer 12

1) If you let one end of flask be exposed to air, so dust particles could get in, while the other end of swan neck is not exposed. 2) COME BACK!!

Answer 13

Several French asked Pasteur to determine why beer and wine sour and spoil -Pastuer discovered that when making beer and wine, YEAST (Not air) converted the sugars to alcohol by a process called FERMENTATION - Also discovered that beer and wine spoiled when different organisms (bacteria), lin the presence of air converted the alcohol to Acetic Acid (Vinegar) ex: acetic acid bacteria

Answer 14

Spoilage solution: PASTEURIZATION (1864) -Pasteur demonstrated that Most of these spoilage bacteria could be killed by heating below the evaporation point of the alcohol in wine/beer Pasteurization - the application of a high heat for a short time -two primary methods (63 degrees C/ 30 mins, 73 degrees C /16 sec)

Answer 15

Pasteurization aims to DESTROY pathogens and Reduce the number of spoilage bacteria

Answer 16

PASTEURIZATION is more effective at killing bacteria in liquids, because at a higher temperature (Boiling) you will kill more bacteria, but it will ruin the taste of food. COME BACK to this 2) If you boil, you will boil off the alcohol, which will alter the properties of foods. You tend to lose quality or taste of foods.

Answer 17

Pasteur believed that "establishing a connection between food spoilage and microbes was a major step toward establishing the relationship between disease and microbes -bacteria may cause diseases in human

Answer 18

Germ Theory of Disease: proposed that certain microorganisms cause disease

Answer 19

Observations that led to Germ Theory Diseased: -1835 and 1865: it was shown that silkworm diseases could be caused by a fungus and protozoan respectively -1840s: Hungarian physician Ignaz Semmelweis showed that fever death rates following childbirth could be dropped from 20% to less than 1% by hand disinfection from one OB patient to another (ideas largely REJECTED) 1860s: English surgeon Joseph Lister was a pioneer of antiseptic surgery (5% phenol; used to wash down surface ) to prevent wound infections -Conclusive proof of theory first came from ROBERT KOCH in 1876

Answer 20

1876: A German physician, Robert Kotch, proved that a bacterium causes anthrax and provided Koch's postulates. Koch's postulates were experimental steps used to prove that a specific microbe causes a specific disease (anthrax found in cattle; bacterial illness from spore bacterium) bacillus anthracs

Answer 21

Koch's Postulates Demonstrating the pathogenicity of a microorganism 1. Microorganism must be observed in every case of the disease 2. It must be isolated and grown in pure culture 3. The pure culture, when inoculated in animals, must reproduce the disease 4. Microorganism must be recovered from the diseased animal .

Answer 22

1796: Edward JENNER inoculated (injected) a person with cowpox virus, who was then protected from small pox 2) -Discovery: Jenner noticed that milk maids who had gotten cowpox virus, were also protected from small pox. Hence he used that as a way to protect people from getting small pox, by injecting others with cowpox. Vaccination is derived from Vacca for cow The protection is called IMMUNITY -This occurred 70 years BEFORE Kotch established the a specific organism causes anthrax 3) Jenner's vaccine: Jenner took material from cowpox sore on milkmaid's (had cowpox) hand and inoculated it into 8 year old boy James Phipps' s arm. James soon recovered from that and then Jenner inoculated James with some of small pox virus and saw no small pox disease developed in James; hence vaccine a success

Answer 23

Types of Vaccines include: -Related avirulent strains -Live attenuated strains (same virus that causes disease, but almost devoid of pathogenicity, not really infectious ) -Heat or chemical killed virulent strains -Isolated proteins components of virulent strains -Nucleic acids (DNA or RNA) expressing protein components of virulent strains

Answer 24

The First Golden Age of Microbiology: -1857-1914 (approx 60 years) -Begins with Pasteur's work on fermentation, period of RAPID ADVANCES in the science of microbiology occurred (Pasteurization, antibiotics, germ theory, vaccination) -This propelled Microbiology into becoming one of the main branches of biology and medicine

Answer 25

The First Golden Age led to new branches of Microbiology: -Bacteriology- is the study of bacteria -Mycology- is the study of Fungi -Virology - is the study of Viruses -Parasitology- is the study of protozoa and Parasitic worms -Immunology- is the study of immunity

Answer 26

BACTERIOLOGY (bacteria and archaea)

Answer 27

First Golden Age of Microbiology (from 1857-1930s) -"The Science of Microbiology rapidly advances and a relationship between microorganism and disease Is established" Second Golden Age of Microbiology (from 1940s- 1980s) - " A search or substances that could destroy pathogens without harming human cells and the birth of molecular genetics"

Answer 28

The Birth of Modern Chemotherapy -Once a relationship between microbes and disease was established, microbiologists searched for substances that killed pathogen without harming host -CHEMOTHERAPY- Treatment of infectious disease using chemicals -By Paul Ehrlich/ antimicrobial chemotherapy 1) Synthetic drugs- are agents produced from chemicals in the lab 2) Antibiotics- MULTIPLE Definitions a) Strict definition: agents produced NATURALLY by bacteria and fungi and act against other bacteria b) Broad definition: agents used to treat MICROBIAL infections

Answer 29

Semi-synthetic drugs- take naturally occurring antibiotic or drug and modify it (through chemical reactions) ex: ampicillin- semi-synthetic drug -Other terms: antimicrobial, antibacterial, antifungals, antivrials

Answer 30

QUININE- first drug in Europe's medical arsenal -Quinine occurs naturally in Cinchona tree bark and was the First effective treatment for (17th century) for Malaria caused by Plasmodium falciparum -it is an antimicrobial (broad definition) and a Natural drug -SELTZER (tonic) also has quinine that can be put into soft drinks

Answer 31

The First Synthetic Drugs: -Paul Ehrlich, German physician coined the term "magic Bullet"- a drug that could destroy a pathogen without harming the host -Observed that some dyes stained bacterial but NOT animal cells and thought it might be possible to develop toxic dyes that would bind and kill bacterial cells without harming animal cells -1910: developed a synthetic arsenic drug, SALVARSAN (Ehrlich 606), to treat syphilis 1930s: the burgeoning dye industry in his country prompted a German microbiologist to search for dyes for antimicrobials -in 1932, a dye with remarkable effects on stopping some bacterial infections in mice were found . It wad found that SULFANILAMIDE, and NOT the dye itself, killed bacteria. This class of drugs were called Sulfonides or "Sulfa Drugs" Sulfonide functional group (R1-S=0 =0-N-R3-R2)

Answer 32

The vast MAJORITY of bacteria are HARMLESS to humans and a large number are actually beneficial -Early microbiologists focused on pathogenic microbes -in the 1880s, Martinus Beijernick and Sergei Windogradsky were two of the first scientists that attempted to understand microbes outside of the medical context; their studies led to the field of microbial ecology *Microbial ecology: the relationship of microorganisms with their environment, each other and plant and animal species

Answer 33

Fortunate accident- ANTIBIOTICS -1928: Alexander FLEMING accidently discovered the first antibiotic it occurred after Fleming went on vacation and came back to observe petri dish; noticed that it had mold and staph bacteria. However, he noticed that the mold inhibited growth of bacteria -Fleming observed that a Penicillium fungus made an antibiotic, called penicillin that killed bacteria 1940s: Penicillin was tested clinically and mass produced Problems associated with antimicrobial drugs: RESISTANCE

Answer 34

More Recent Fields of Biology; 1) Microbial genetics: the study of how Microbes inherit traits 2) Molecular Biology- the branch of biology that deals with Structure and function of the macromolecules (ex: proteins and nucleic acids) essential to life -**this overlaps with other areas of biology and chemistry, particularly genetics and biochemistry 3) Genomics: The study of an organisms genes; has provided new tools for classifying microorganisms -(used DNA sequence to compare sequencers of other bacteria, see how closely related to each other )

Answer 35

prior to 1940s, genetic research was based on plants on animals -Unicellular microorganism (Bacteria/yeast) are advantageous for genetic and biochemical research: -Less complex than animals and plants -Life cycles of most bacteria require less than an hour, allowing the cultivation of large numbers of cells in short period of time (faster cycle) -Well defined genetic systems can easily modify genes that facilitate studies -Haploid genome (1 set of chromosomes) allows mutant analysis to be easy (only have to knock out one copy of gene to see phenotype of mutation)

Answer 36

Third Golden age of Microbiology (now): -"advances in genomics and biotechnology help us identify genes and their function

Answer 37

-Current time period considered the Golden age of classification because we use DNA/gene sequencing to compare, and it allows us to know how bacteria is related.

Answer 38

First organized team to optimize the biological activity of a lead compound through systemic chemical modification: A toxic organic compound was chosen as a precursor chemical. Hundreds of related organoarsenic compounds were synthesized, Each was tested for toxicity, and biological activity in rabbits, infected with the syphilis-causing bacteria (Treponema palladium), Salvarsan was the best candidate. SELECTIVE TOXICITY was far from absolute -still toxic and ocassionally killed patients -salvarsan- worked for many patients with syphilis, and did not kill some patients

Answer 39

Beneficial environmental activities of microbes: 1. Recycling vital elements 2. Bioremediation 3.Insect pest control 4. Biotechnology

Answer 40

Recycling Vital elements: -C, N, O, S, P are abundant but not necessarily in useful forms. *Microorganism are primarily responsible for converting these elements to forms useful for plants and animals Producers: *ALGAE, Cyanobacteria, and higher plants use CO2 to p produce carbohydrates for animals, fungi, and bacteria (for energy) -N2 is abundant but not in a form usable by plants and animals -ONLY BACTERIA can naturally "fix" atmospheric nitrogen Decomposers: Microorganisms, primarily* bacteria and fungi*, play a key role in returning CO2 to the atmosphere

Answer 41

Bioremediation -Any process that uses organisms or their enzymes to clean up pollutants and toxic wastes -Bacteria decompose organic material in sewage treatment plants to recycle water -Bacteria DEGRADE or DETOXIFY pollutants such as oil, chemicals and heavy metals *Bacterial enzymes (instead of chemicals) added to drain cleaners and detergents -*Indigenous or genetically modified bacteria

Answer 42

Insect pest control: -Insect pathogens are Alternatives to chemical pesticides -B. thuringiensis produces a CRYSTAL protein during sporulation toxic to the digestive system of many insects, but harmless to plants and animals -Incorporated into dusting powder or spray and requires ingestion -targets several insect Orders, types specific to an insect family -Bt (Bacillus Thuringiensis) transgenic plants have been available since late 1990s -Bt transgenic plants- take a gene (DNA) from bacteria (Bacillus.t) , put it into another plant so it will form a spore and toxic crystal that will keep pests or insects away from crops

Answer 43

Biotechnology: the industrial application of microbes, cells or cell components to make a useful products -Although, the use of microbes to produce foods and chemicals is centuries old, techniques have become much more sophisticated in past few decades ex: use of microbes in making cheese -Milk has formed curd and has been inoculated with ripening bacteria for flavor and acidity -The curd ish then chopped into cubes, for draining of whey and will be milled and compressed to form cheese.

Answer 44

Biofilms- a complex aggregation of microorganism growing on a solid surface ex: rocks, pipes, teeth, and medical implants -Characterized by structural heterogeneity, genetic diversity, complex community interactions, and an Extracellular matrix of polymeric substances (EPS) EPS- Extracellular matrix of polymeric substances aid cohesion of microorganism and adhesion of biofilms to surfaces and aid with interactions between microorganisms ex: biofilm seen on catcher or contact lens case

Answer 45

Benefits of Biofilms: -Protect your mucus membrane from harmful microbes -food for aquatic animals in lakes -Can be harnessed for constructive purpose (ex: sewage treatment) Harmful Effects of Biofilms: -Can clog water pipes -can colonize medical implants and cause infections -Bacteria in biofilms have increased antibiotic resistance -Dental plaque can lead to tooth decay

Answer 46

Infectious Disease: a disease that occurs when a pathogen overcomes the host's resistance

Answer 47

Emerging Infectious Diseases (EIDs): New diseases and diseases increasing in incidence

Answer 48

Factors contributing to EIDs (Emerging Infectious Diseases): 1. Evolutionary changes in existing organisms -Increased pathogenicity (ex: Escherichia coli O157:H7) O: sugar exposed outside of cell; H: protein in flagella -change in pathogen's ecology (Powassan virus becoming establisher in the same deer ticks that cause Lyme disease; vectors) 2. Changes in weather patterns (rain, drought) 3. Ecological disster (lot of flooding, water contaminated) 4. Innapropriate use of antibiotics (increase resistance ) 5.Close interactions with wild animals, livestock (infection) 6. Spread of known diseases to new geographic regions by modern transportation (ex; West Nile virus) 7. Urbanization, expanding human settlement 8. War, Poverty 9. Animal habitat change 10. Ineffective or mistaken public health measures 11. Technological advances (ex: Legionella Pneumonia dispersed in mist from hot tubs, showers, air-conditioning systems) 12. Cultural changes (Toxic-Shock Syndrome: introduction of high absorbency tampons caused overgrowth of S. aureus and release of toxins from overgrowth; measles) 13. Use of microbes as weapons (ex: dispersal of Bacillus anthraces spores)

Answer 49

Avian Influenza A (bird flu) -Influenza A virus (H5N1) -Primarly in waterfowl and poultry (epizootic-killed tens of millions of birds since 2003 in Asia, Europe and Africa) -Person to person transmission has been very RARE, limited and unsustained 2022 cases: Poultry 43,851,907/ human 1

Answer 50

Swine Flu -in 2009, a new subtype of (H1N1) resulting from the reassortment of human and swine influenza viruses appeared -in 2009, Swine flu was the MOST COMMON cause of flu in humans; declared a pandemic and caused 17,000 deaths (Covid-19, 6.5 Million Deaths) -Still circulates in humans as seasonal flu virus - H1N1 (Swine flu) is a type A influenza virus that affects pigs (swine) -it can pass to humans through contact or contamination Human swine flu symptoms include fever, nausea, sore throat Swine flu outbreak in India, (2014-2015) resulted in 2,335 deaths

Answer 51

Influenza viruses are divided into subtypes based on two proteins on the surface of the virus: Hemagglutinin (H) and Neuraminidase (N) (H) enables the virus to BINDcells of the upper respiratory tract containing the sugar sialic acid on their membranes. It also allows FUSION of the viral envelope with the endoscome membrane - (N) CLEAVES sialic acid side groups and is essential for RELEASE of progeny virus particles from the surface of an infected cell

Answer 52

Staphylococcus aureus- ANTIBIOTIC-RESISTANT Infection -The bacteria are generally harmless, unless they enter the body through a cut or other wound, and even then they usually cause only minor skin problems in healthy people -However, it can be an OPPORTUNISTIC pathogen causing a wide range of infections (pimples. boils, pneumonia, food poisoning, surgical wound infection) and is a significant cause of hospital-associated infections

Answer 53

Antibiotic-Resistant infections -Stapholococcous aureus -1950s: Penicillin resistance developed 1980s: Methicillin resistance (Methicillin- semi-synthetic drug; modified version of penicillin) -MRSA: Methicillin-resistant S. aureus -1990s: MRSA resistance to vancomycin reported -VISA: Vancomycin- intermediate-resistant S. aureus -VRSA; Vancomycin- resistant S. aureus (2002) Vancomycin (peptide antibiotic)

Answer 54

Healthcare-associated MRSA infections are the MOST COMMON and occurs in people who have been in hospitals or other health care settings, such as nursing homes and dialysis centers Community-associated MRSA occurs in WIDER Communtiy- among healthy people. At risk populations include groups such as high school wrestlers, child care workers, and people who live in crowded conditions Multiple S. aureus strains have been isolated from HA-MRSA and CA-MRSA infected patients. Some are HA- or CA-MRSA specific while others have been isolated from both patients.

Answer 55

Multidrug-resistant Tuberculosis (MDR-TB): Tuberculosis is caused by Mycobacterium Tuberculosis that is RESISTANT t to at least two of the most potent TB drugs, Isoniazid and Rifampin

Answer 56

Clostridium difficile (bacteria)

Answer 57

Clostridium difficile- is the MOST COMMON cause of hospital-acquired infections -Epidemic C. difficile produce More TOXINS and are more RESISTANT to antibiotics difficile- difficult to grow in culture

Answer 58

Ebola virus Disease (formerly known as Ebola Hemorrhagic fever) -Ebola virus causes fever, hemorrhaging and blood clotting -First identified (1976) near Ebola River, Congo -Outbreaks occur every few years (2 outbreaks in 2021) -50-90% death rate -Transmitted human to human via blood/body fluids/tissue

Answer 59

Ebola Virus Disease -ERVEBO is currently the only FDA-approved vaccine (12/19) available to prevent EVD. It doesn't contain the whole virus. Rather, it is a VIRAL VECTOR carrying a single gene specific to the Zaire strain of ebolavirus -several other vaccines are in development

Answer 60

Marbug virus -another HEMORRHAGIC Fever virus with clinical symptoms almost indistinguishable from Ebola Hemorrhagic fever -first noticed during small outbreaks in several German cities (ex: Marburg) in the 1960s (began with lab workers that handled African green monkeys) -Most outbreaks in African (NO vaccine) -RARE: 13 outbreaks in Africa since 1975 killing 1 to 252 people -Transmitted human to human like EVD, but has also occurred by handling ill or dead infected green monkeys and fruit bats

Answer 61

African Fruit bats are the Natural reservoir for Marburg virus. Although not proven, bats are the likely reservoir for EBOLA virus as well

Answer 62

Zika Virus disease: -Although discovered in 1947, in the Zika forest of Uganda, it didn't make world headlines until 2005 -Virus is spread to people primarily through the bite of an infected AEDES species mosquito -Many people infected with the virus won't have symptoms or will only have mild symptoms However, the Virus can pass from a pregnant woman to her fetus during pregnancy or around time of birth and is linked to problems, such as miscarriage, stillbirth, and birth defects since 2017- No current local transmission of Zika virus have been reported in the continental United States -In 2016, and 2017, local mosquito-borne transmission of Zika was reported in FLORIDA (N= 220) AND Texas (N= 11)

Answer 63

Middle East Respiratory Syndrome (MERS) -Respiratory illness also known as CAMEL flu (believed camels involved in spread but unclear how) -Caused by a new member of the coronavirus family called Middle East respiratory syndrome coronavirus (MERS-CoV) -first reported in Saudi Arabia in 2012, spread to Europe and Asia, and two travel-associated cases occurred in US in 2014

Answer 64

Advertisements tell you that bacteria and viruses are all over your home and that you need to buy antibacterial cleaning products. -You Should NOT buy antibacterial products; you can wash your hands

Answer 65

In September 2016, the FDA announced that effective in September 2017, it would PROHIBIT the sale of non-medical soap containing triclosan or 18 other ingredients marketd as antimicrobials due to FDA's findings of the lack of efficacy in these products

The Microbial World and You Flashcards

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