Thermal DNA Denaturation Flashcards
(5 cards)
1
Q
The midpoint of melting (Tm) depends on
A
Base composition
2
Q
High CG
A
Increases Tm= more hydrogen bonds
3
Q
Tm depends on
A
Dna length
4
Q
Longer DNA =
A
Higher Tm
5
Q
Tm depends on pH and
A
Ionic strength
HIGH SALT🍟 increase Tm
