thin layer chromatography Flashcards
(12 cards)
what is visualisation of TLC
where somethin needs to be done to temporarily see the comounds
uses of TLC
to assess reaction progress to determine if reactants have been consumed to form a new product
what is a co spot
where reactant and product are spotted on top of each other
what is the retention factor
how chemists report the result of TLC plate , as R value, where the Rf = distance travelled by the compound/distance travelled by the solvent
what does an Rf value of 0.55 mean
the spot moved 55% as far as the solvent front
what is the order of elution on silica TLC plates
less polar is higher, more polar is lower
silica attracts polar compounds
describe betweeb benzyl alcohol, benzaldehyde and ethylbenzene which will elute from TLC plate the quickest
benzyl alcohol = most polar - retained the most
benzaldehyde = moderately polar so can be carried further by the mobile phase
ethylbenzene = least polar - travels with mobile phase
what are the functional groups polarity
increasing n polarity
alkanes
alkenes
aromatic hydrocarbons
ethers, halocarbons
aldehydes, ketones, esters
amines
alcohols
carboxylic acids
is amide or amine more polar
amide is more polar, bc it can hydrogen bond and accept hydrogen bonds on oxyden and nitrogen
structure of amide group?
has a carbonyl group bonded to nitrogen atom