TLC Flashcards

1
Q

chromatography is a method for…

A
  • identification
  • isolation
  • purification
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2
Q

what is the main function for thin layer chromatography

A

comparing/identifying unknown compounds

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3
Q

what is the stationary phase and is it polar or nonpolar

A

the stationary phase is the coating on the hard back of the TLC plate. The stationary phase is what the mobile phase adhears to as it carries the sample up the TLC plate.

the stationary phase is polar

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4
Q

what is the mobile phase and is it polar or nonpolar

A

the mobile phase is the solvent that the sample is dissolved in. the mobile phase carries the samples up through the stationary phase (coating)

the mobile phase is nonpolar

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5
Q

what does it mean if a dot is lower on the TLC plate

A

the compound in that dot is more polar, so it has a higher affinity for the polar stationary phase

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6
Q

what does it mean if a dot is higher on the TLC plate

A

the compound in that dot is more ononpolar and has a higher affinity for the nonpolar mobile phase

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7
Q

what ha[[ens if the solvent (mobile phase) is too nonpolar or too polar

A

the dots will not separate

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8
Q

what happens if the solvent is too polar

A

the dots will move too far up the TLC plate

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9
Q

what happens if the solvent is too nonpolar

A

the dots wil hardly move off the baseline

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10
Q
A
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