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Molecules to Medicine Unit 1 > Tools of Molecular Biology > Flashcards

Flashcards in Tools of Molecular Biology Deck (14):
1

Restriction sites are usually ____________

Palindromes

2

The basis of diagnosing disease with restriction fragment length polymorphisms (RFLP) is that a disease allele has to lead to a loss or a gain of a _____________ for ____________

Cleavage site for restriction endonuclease

3

DNA fingerprinting is used in ______________ by examining ___________

Paternity testing; variable number of tandem repeats

4

Southern blotting is used to identify ___________ and __________ is used to transfer from electrophoresis gel to the membrane.

DNA; radioactive DNA probe

5

Northern blotting is used to identify ______________

RNA fragments

6

Annealing the probe to the template at the temperature just below the ___________ is essential for specific hybridization and preventing non-specific hybridization.

melting temperature of the primer of the probe

7

Plasmids are the vector of choice for amplification of DNA in ____________

Bacteria

8

Bacteriophages are used as vectors when ______________ of the introduction of the recombinant vector is required

High efficiency

9

___________ are hybrids of plasmids and bacteriophages. They will not kill their host cell and use a plasmid replication origin.

Cosmids

10

_________ are used where integration into the host genome is required.

Viral Vectors

11

In microarrays, there are order array of oligonucleotides primers that hybridize to DNA and RNA targets. They are flouresantly labeled so the level of RNA or DNA in the sample is inferred from the ___________

Signal intensity

12

What is recombinant DNA?

DNA molecules produce artificially

13

What does transformation mean?

When bacterial cells are made competent to take up plasmid DNA

14

In PCR, the DNA strand is denatured by heating to _______, then the solution is cooled to _______ which allows primers to hybridize to the template. Finally, the polymerase uses each strand as a template and make a copy by extending the 3' end of the primer at around __________

95 degrees, 55 degrees, 72 degrees