Flashcards in Tools of Molecular Biology Deck (14):
Restriction sites are usually ____________
The basis of diagnosing disease with restriction fragment length polymorphisms (RFLP) is that a disease allele has to lead to a loss or a gain of a _____________ for ____________
Cleavage site for restriction endonuclease
DNA fingerprinting is used in ______________ by examining ___________
Paternity testing; variable number of tandem repeats
Southern blotting is used to identify ___________ and __________ is used to transfer from electrophoresis gel to the membrane.
DNA; radioactive DNA probe
Northern blotting is used to identify ______________
Annealing the probe to the template at the temperature just below the ___________ is essential for specific hybridization and preventing non-specific hybridization.
melting temperature of the primer of the probe
Plasmids are the vector of choice for amplification of DNA in ____________
Bacteriophages are used as vectors when ______________ of the introduction of the recombinant vector is required
___________ are hybrids of plasmids and bacteriophages. They will not kill their host cell and use a plasmid replication origin.
_________ are used where integration into the host genome is required.
In microarrays, there are order array of oligonucleotides primers that hybridize to DNA and RNA targets. They are flouresantly labeled so the level of RNA or DNA in the sample is inferred from the ___________
What is recombinant DNA?
DNA molecules produce artificially
What does transformation mean?
When bacterial cells are made competent to take up plasmid DNA