topic 2 : cells, viruses and reproduction of living things Flashcards
(46 cards)
compare and contrast the ultrastructure of eukaryotic cells and prokaryotic cells
similarities
- both have cell membranes
- sme eukaryotes like bacteria also have cell wall
- both have cytoplasm
- both have ribosomes
differences
- eukaryotes have membrane bound organelles
- prokaryotes have plasmid DNA, eukaryotes have a membrane bound nucleus
- eukaryotes have 80s ribosomes, prokaryotes have 70s ribosomes
what is the ultrastructure of prokaryotic cells (eg bacteria)
- cell wall : made of peptidoglycan. for strength and support
- slime capsule : protective layer –> allow cell to retain moisture and adhere to surfaces
- plasmid : circular piece of DNA
- flagellum : helps the cell move
- pili : hair-like structure –> receptors that allow plasmids to move from cell to cell
- 70s ribosomes : site of protein synthesis
- mesosomes : contain enzymes for respiration
- mitochondria : energy
what is the ultrastructure of eukaryotic cells
- nucleus : DNA
- RER : rer has ribosomes on surface. folds and process proteins (protein synthesis)
- SER : a system of membrane-bound sacs. The SER synthesises and processes lipids and steroids
- golgi apparatus : processes proteins and lipids (also produces lysosomes)
- mitochondria : site of aerobic respiration
- centrioles : involved in cell division
- 80S ribosomes : site of protein synthesis
- lysosomes : contain digestive enzymes
difference in structure between gram positive and gram negative plants
Gram positive bacteria have a thick peptidoglycan layer and no outer lipid membrane whilst Gram negative bacteria have a thin peptidoglycan layer and have an outer lipid membrane.
how to distinguish gram positive and gram negative bacteria
gram staining occurs :
- crystal violet used to stain over a fixed culture, which binds to peptidoglycan cell wall.
- after, stain poured off and slide is rinsed with water.
- iodine solution added and removed
- alcohol added (lipopolysaccharides are soluble in alcohol, so gram neg membrane dissolves)
- counterstain red safranin - gram neg is pink/red, gram positive is purple (trap the stain because of thick peptidoglycan layer)
describe how to use a micrometer to determine how many times bigger a drawing is than the actual cell in the blood smear
- measure the length of the blood cells using an eye piece/stage in micrometer
- divide the length by the magnification of object
- more than one measurement taken
- divide this value into the length of the blood cells in the drawing
magnification equation
image = actual size x magnification
advantages and disadvantages of electron microscope
adv :
- better resolution (more detailed pictures)
disadv :
- expensive
- can’t look at living material
- large
what are the ethical implications on using or not using untested drugs
- difficult to obtain informed consent
- unknown side effects
- may not be as effective as the currently accepted treatment
rna retrovirus structure
- have lipid envelope
- single strand of viral RNA : use reverse transcriptase to make viral dna
dna virus structure
- contain viral DNA : used as direct template for new viral DNA and for mRNA to make viral proteins
- eg. bacteriophages including lambda phage,
eg. small pox
rna virus structure
- much more likely to mutate
- majority contain single strand of `RNA (ss rna)
eg. tobacco mosaic virus, sars, polio
how does the rna retrovirus replicate and infect the host cell
and give some examples
- viral rna enters host cell - cannot be used as MRNA
- RNA translated to viral DNA by reverse transcriptase in cytoplasms
- viral DNA incorporated into host DNA.
- production of viral dna and proteins
- viral dna remains in host so continues to make more virus particles
eg. HIV
difference between positive and neg ssrna
- positive ssRNA : RNA acts directly as MRNA and is translated
- neg ssRNA : RNA is transcribed and then translated
how to control viral infections
antiviral drugs
- target receptors on virus –. prevet them from recognising host cell
- target enzyme of host cell used to make DNA/RNA
- inhibit protease enzyme (which allows the virus to be hidden)
vaccine : aim to provide herd immunity (dead/inactive pathogens that stimulate an immune response)
how to control viral infections
antiviral drugs
- target receptors on virus –. prevet them from recognising host cell
- target enzyme of host cell used to make DNA/RNA
- inhibit protease enzyme (which allows the virus to be hidden)
vaccine : aim to provide herd immunity (dead/inactive pathogens that stimulate an immune response)
how to reduce viral transmissions
- a lockdown
- good hygiene/disinfection
- distance (stop airborne viruses)
- sterilize medical equipment
what is the lysogenic cycle
- bacteria attach to bacterium
- inject dna into the host cell and synthesise viral enzymes
- viral dna incorporated in host cells
- the viral dna replicates each time (doesnt cause damage)
REMAIN DORMANT
what is the lytic cycle
(after change in environment, cycle goes from lysogenic to lytic)
- virus DNA take over biochem of cell
- DNA (viral) replicated
- viral proteins synthesised
- the cell will lyse and virus infects other cells
describe the stages of the cell cycle
G1 (interphase) : growth, proteins to make organelles
S : replication of DNA
G2 : organelles made, energy store increase and DNA checked for errors
mitosis : cell division
cytokinesis : formation of 2 cells which are genetically identical (cell membrane splits)
what are the checkpoints in the cell cycle
G1 cp : check cell size, nutrients, growth factors and DNA damage
G2 cp : cell size, replication done right? DNA damage check
metaphase cp : chromosomes aligned at equator, spindle fibres form properly
describe what happens in mitosis
- interphase : growth and dna replication, spindle formes
- prophase : centrioles move to poles, chromosomes condense, nucleolus disappears, nuclears membrane breaks down
- metaphase: chromosomes align at the equator. spindle fibres attach to the centromeres
- anaphase : centromere divide, sister chromatids pulls to opposite poles by spindle fibres
- telophase : nuclear membrane reforms around 2 sets of chromatids, chromosomes re forms
what are the products of mitosis
2 genetically identical diploid daughter cells.
describe the process of meiosis
P1 : nuclear membrane breakdown, centrioles move to opposite poles, nucleolus disappears, chromosomes condense. HOMOLOGUS CHROMOSOMES CROSS OVER - exchange genetic information
Metaphase 1:
Chromosome pairs line up at the equator – independent assortment of chromosomes
Spindle fibres attach to the homologous chromosomes
Anaphase 1:
Chromosomes are pulled apart
Telophase 1: nuclear membrane forms around chromatids and spindle fibre disappear, chromosomes decondense
Cytokinesis: produces 2 diploid daughter cells (genetically different)
Prophase 2:
Nuclear membrane breaks down, centrioles move to opposite poles, nucleolus disappears, chromosomes condense
Metaphase 2:
Chromosomes line up at the equator – independent assortment of chromosomes
Spindle fibres attach to the centromeres
Anaphase 2:
centromeres divides chromatids are pulled to opposite poles
Telophase 2 : nuclear membrane forms around chromatids and spindle fibre disappear, chromosomes decondense
Cytokinesis: produces 4 haploid genetically different daughter cells