Topic 2 - Special microscopes

Question 1

Definition;

Answer 1

LM developed for special diagnoctics, or reaserch purposes

Question 2

Types of special microscopes;

Answer 2

1. Stereo M
2. Dark field M
3. Phase contrast M
4. Polarization M
5. Fluroscence M
6. Confocal scanning M
7. Colposcope

Question 3

Stereo M

Answer 3

Used to increase resolution of specimens visible with naked eye.
Surgical M = Stereo M with moving stage

Question 4

Dark filed M

W&W + Usage

Answer 4

Reade, 1840

Demonstration of non-stainable bacteria

Question 5

Phase contrast M

W&W + Usage

Answer 5

Zernika, 1936
Study of non-stained, even living preparates
(ex:cell cultures)

Question 6

Polarization M

W&W + Usage

Answer 6

Talbot, 1829

For membranes, cross-striated muscles and diagnosing illnesses with crystal formation

Question 7

Fluroscense M

W&W + Usage

Answer 7

Reichert, 1911.

Studying autofluroescence or fluroescence immunocytochemistry

Question 8

Confocal scanning

Answer 8

Laser light source.
Scans surface of specimen
Emitted light is detected and used to form computerized image. Allows optical section og whicker samples and 3D reconstruction of tissue components.
Use: Study thicker sections and 3D reconstruction

Question 9

Colposcope

W&W + Usage

Answer 9

Hinselman, 1926

Used for examination of vagina and cervix