topic 3B: mass transport in animals Flashcards

(37 cards)

1
Q

Describe the role of red blood cells & haemoglobin (Hb) in oxygen transport

A

● RED BLOOD CELLS contain lots of HAEMOGLOBIN
○ NO NUCLEUS AND BIONCAVE → more space for haemoglobin, high SA:V & short diffusion distance
● haemoglobin ASSOCIATES with and BINDS loads of oxygen at GAS EXCHANGE SURFACES (lungs) where PARTIAL PRESUURE OF OXYGEN (pO2) is HIGH
● This forms OXYHAEMOGLOBIN which TRANSPORTS oxygen
○ Each can carry FOUR OXYGEN MOLECULE, one at each Haem group
● haemoglobin dissociates from / unloads oxygen near cells / tissues where pO2
is low

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2
Q

Describe the structure of haemoglobin

A

● PROTEIN with a QUATERNARY structure
● Made of 4 POLYPEPTIDE CHAINS
● Each chain contains a HAEM GROUP containing an IRON ION (Fe
2+)
The haemoglobins are a group of CHEMICALLY SIMILAIR molecules found in many different organisms

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3
Q

Describe the loading, transport and unloading of oxygen in relation to the
oxyhaemoglobin dissociation curve - Areas with low pO2 - respiring tissues

A

● Hb has a LOW AFFINITY for oxygen
● So oxygen readily UNLOADS and DISSACIOATES with haemoglobin
● So % saturation is LOW

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4
Q

Describe the loading, transport and unloading of oxygen in relation to the
oxyhaemoglobin dissociation curve - Areas with high pO2 - gas exchange surfaces

A

● haemoglobin has a HIGH AFFINITY for oxygen
● So oxygenreadily LOADS and ASSOCIATES haemoglobin
● So % saturation is HIGH

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5
Q

Explain how the cooperative nature of oxygen binding results in an
S-shaped (sigmoid) oxyhaemoglobin dissociation curve

A
  1. BINDIN of FIRST OXYGEN changes TERTIARY and QUATERNARY STRUCTURE structure of haemoglobin
  2. This UNCOVERS HAEM GROUP BINDING SITES, making FURTHER BINDING of oxygens EASIER
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6
Q

Describe evidence for the cooperative nature of oxygen binding

A

● A low pO2 as oxygen increases there is a SMALL INCREASE IN % SATURATION of haemoglobin with oxygen
○ When first oxygen is binding
● At higher pO2, as oxygen increases there is a BIG RAPID INCREASE in % SATURATION of haemoglobin with oxygen
○ Showing it has got easier for oxygens to bind

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7
Q

Explain effect of CO2 concentration on the dissociation of oxyhaemoglobin

A
  1. Increasing blood CO2 eg. due to increased rate of RESPIRATION
  2. LOWERS blood pH (more acidic)
  3. REDUCING Haemoglobin’s AFFINITY for oxygen as the SHAPE and tertiary / quaternary structure changes slightly
  4. So MORE and FASTER UNLOADING of oxygen to respiring cells at a given pO2
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7
Q

What is the Bohr effect?

A

Effect of CO2 CONCENTRATION on dissociation of oxyhaemoglobin → curve shifts to RIGHT

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8
Q

Describe evidence for the Bohr effect

A

At a given pO2 %, the SATURATION of haemoglobin with oxygen is LOWER

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9
Q

Explain the advantage of the Bohr effect (eg. during exercise)

A

More dissociation of OXYGEN → faster AEROBIC RESPIRATION and less anaerobic respiration → more ATP produced

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10
Q

Explain why different types of haemoglobin can have different oxygen
transport properties

A

● Different types of Hb are made of polypeptide chains with slightly DIFFERENT AMINO ACID SEQUENCES
● Resulting in different TERTIARY AND QUATERNARY STRUCTURES and shape
● So they have different AFFINITIES for oxygen

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11
Q

Explain how organisms can be adapted to their environment by having
different types of haemoglobin with different oxygen transport properties - Curve shift left
Hb has higher affinity for O2

A

● MORE O2 ASSOCIATES with Haemoglobin MORE READILY
● At GAS EXCHANGE SURFACES where pO2
is LOWER
● Eg. organisms in LOW O2 environments - HIGH ALTITUDES, UNDERGROUND OR FOETUSES

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12
Q

Explain how organisms can be adapted to their environment by having
different types of haemoglobin with different oxygen transport properties - Curve shift RIGHT Haemoglobin has LOWER affinity for O2

A

● MORE O2 DISSOCIATES from haemoglobin MORE READILY
● At RESPIRING TISSUES where more O2
is needed
● Eg. organisms with HIGH RATES OF RESPIRATION AND METABOLIC RATE (may be small or active)

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13
Q

Describe the general pattern of blood circulation in a mammal

A

CLOSED DOUBLE CIRCULATORY SYSTEM - blood passes through heart TWICE for every circuit around body:
1. DEOXYGENATED blood in RIGHT side of heart pumped to LUNGS; oxygenated returns to left side
2. OXYGENATED blood in LEFT side of heart pumped to REST OF BODY; deoxygenated returns to right

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14
Q

Suggest the importance of a double circulatory system

A

● Prevents MIXING of OXYGENATED AND DEOXYGENATED blood
○ So blood pumped to body is FULLY SATURATED with oxygen for AEROBIC RESPIRATION
● Blood can be pumped to body at a HIGHER PRESSURE (after being lower from lungs)
○ Substances taken to / removed from body cells QUICKER AND MORE EFFICIENTLY

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15
Q

Name the blood vessels entering and leaving the heart and lungs

A

● VENA CAVA – transports deoxygenated blood from respiring body tissues → heart
● PULMONARY ARTERY – transports
deoxygenated blood from heart → lungs
● PULMONARY VEIN – transports oxygenated blood from lungs → heart
● AORTA – transports oxygenated blood
from heart → respiring body tissues

16
Q

Name the blood vessels entering and leaving the kidneys

A

● RENAL ARTERIES – oxygenated blood → kidneys
● RENAL VEINS – deoxygenated blood to vena cava from kidneys

17
Q

Name the the blood vessels that carry oxygenated blood to the heart muscle

A

CORONARY ARTERIES - located on surface of the heart, branching from aorta

18
Q

Suggest why the wall of the left ventricle is thicker than that of the right

A

● Thicker muscle to CONTRACT with greater force
● To generate HIGHER PRESSURE to pump blood around ENTIRE BODY

19
Q

Explain the pressure & volume changes and associated valve movements
during the cardiac cycle that maintain a unidirectional flow of blood - Atrial systole

A

● Atria CONTACT and ventricles relax
● So their volume DECREASES,
pressure INCREASES
● ATRIOVENTRICULAR VALVES OPEN
when pressure in ATRIA EXCEEDS pressure in VENTRICLES
● SEMILUNAR VALVES remain SHUT
as pressure in ARTERIES EXCEEDS pressure in VENTRICLES
● So blood pushed into VENTRICLES

20
Q

Explain the pressure & volume changes and associated valve movements
during the cardiac cycle that maintain a unidirectional flow of blood - Ventricular systole

A

● Ventricles CONTRACT
● So their volume DECREASES,
pressure INCREASES
● ATRIOVENTRICULAR VALVES SHUT
when pressure in VENTRICLES EXCEEDS pressure in ATRIA
● SEMILUNAR VALVES OPEN when
pressure in VENTRICLE EXCEEDS
pressure in ARTERIES
● So blood pushed out of heart
through ARTERIES

21
Q

Explain the pressure & volume changes and associated valve movements
during the cardiac cycle that maintain a unidirectional flow of blood - Diastole

A

● Atria & ventricles RELAX
● So their volume INCREASES,
pressure DECREASES
● SEMILUNAR VALVES SHUT when
pressure in ARTERIES EXCEEDS pressure in VENTRICLES
● ATRIOVENTRICULAR VALVES OPEN when pressure in ATRIA EXCEEDS pressure in VENTRICLES
● So blood fills ATRIA via veins &
flows passively to VENTRICLES

22
Q

Explain how graphs showing pressure or volume changes during the cardiac
cycle can be interpreted, eg. to identify when valves are open / closed
-semilunar valves closed
-semilunar valves opened
-atrioventricular valves closed
-atrioventricular valves open

A

Semilunar valves closed
● Pressure in [named] ARTERY HIGHER than in VENTRICLE
● To PREVENT BACKFLOW of blood from artery to ventricles

Semilunar valves open
● When pressure in VENTRICLE is HIGHER than in [named] ARTERY
● So blood flows from VENTRICLE to ARTERY

Atrioventricular valves closed
● Pressure in VENTRICLE HIGHER than ATRIUM
● To PREVENT BACKFLOW of blood from ventricles to atrium

Atrioventricular valves open
● When pressure in ATRIUM is HIGHER than in VENTRICLE
● So blood flows from ATRIUM TO VENTRICLE

23
Q

How can heart rate be calculated from cardiac cycle data?

A

Heart rate (beats per minute) = 60 (seconds) / length of ONE CARDIAC CYCLE (seconds)

24
Describe the equation for cardiac output
CARDIAC OUTPUT (volume of blood pumped out of heart per min) = STROKE VOLUME (volume of blood pumped in each heart beat) x HEART RATE (number of beats per min)
25
Explain how the structure of arteries relates to their function
Function – carry blood AWAY from heart at HIGH PRESSURE THICK smooth MUSCLE tissue: Can CONTRACT and CONTROL AND MAINTAINS AND WITHSTAND BLOOD FLOW AND PRESSUSRE THICK ELASTIC tissue: Can STRETCH as ventricles contract and RECOIL as ventricles relax, to reduce PRESSURE SURGES AND EVEN OUT blood pressure AND MAINTAINS HIGH PRESSURE THICK wall: WITHSTANDS high pressure / prevents BURSTING SMOOTH AND FOLDED endothelium: Reduces FRICTION and can stretch NARROW lumen: INCREASES AND MAINTAINS high pressure
26
Explain how the structure of arterioles relates to their function
Function – (division of arteries to smaller vessels which can) DIRECT BLOOD TO DIFFERENT CAPILLARIES AND TISSUE ● THICKER smooth MUSCLE layer than arteries ○ CONTRACTS → NARROWS lumen (VASOCONSTRICTION) → REDUCES BLOOD FLOW to capillaries ○ RELAXES → WIDENS lumen (VASODILATION) → INCREASES BLOOD FLOW to capillaries ● THINNER ELASTIC layer → pressure surges are lower (as further from heart / ventricles)
27
Explain how the structure of capillaries relates to their function
FUNCTION - allow efficient EXCHANGE of substances between blood and tissue fluid (EXCHANGE SURFACE) Wall is a THIN (one cell) layer of endothelial cells: Reduces DIFFUSION DISTANCE Capillary bed - LARGE NETWORK of branched capillaries: Increases SURFACE AREA for diffusion SMALL DIAMETER and narrow lumen: REDUCES BLOOD FLOW RATE so more TIME for diffusion PORES in walls between cells: Allow larger substances through
28
Explain how the structure of veins relates to their function
FUNCTION– carry blood BACK TO HEART at LOWER PRESSURE ● WIDER LUMER than arteries → less resistance to blood flow ● VERY LITTLE elastic and muscle tissue → blood pressure lower ● VALVES → prevent BACKFLOW of lood
29
Explain the formation of tissue fluid
At the ARTERIOLE END of capillaries: 1. HIGHER BLOOD AND HYDROSTATIC PRESSURE inside capillaries (due to contraction of VENTRICLES) than tissue fluid (so net outward force) 2. FORCING water (and dissolved substances) out of capillaries 3. LARGE PLASMA PROTEINS remain in capillary
30
Explain the return of tissue fluid to the circulatory system
At the VENULE END of capillaries: 1. Hydrostatic pressure REDUCES as fluid leaves capillary (also due to friction) 2. (Due to water loss) an INCREASING CONCENTRATION of plasma proteins LOWERS WATER POTENTIAL in capillary below that of tissue fluid 3. Water enters capillaries from tissue fluid by OSMOSIS down a WATER POTENTIAL GRADIENT 4. Excess water taken up by LYMPH CAPILLARIES and returned to circulatory system through veins
31
Suggest and explain causes of excess tissue fluid accumulation
● LOW concentration of PROTEIN in blood plasma ○ Water potential in capillary not as low → WATER POTENTIAL GRADIENT is REDUCED ○ So MORE tissue fluid FORMED at arteriole end and LESS WATER ABSORBED at venule end by osmosis ○ LYMPH system may not be able to drain excess fast enough ● High BLOOD pressure (eg. caused by HIGH SALT CONCENTRATION ) → high HYDROSTATIC pressure ○ INCREASES OUTWARD PRESSURE from arteriole end AND REDUCES INWARD PRESSURE at venule end ○ So MORE tissue fluid FORMED at arteriole end and LESS WATER ABSORBED at venule end by osmosis ○ LYMPH system may not be able to drain excess fast enough
32
What is a risk factor? Give examples for cardiovascular disease
● An aspect of a person’s LIFESTYLE or substances in a person’s BODY AND ENVIRONMENT ● That have been shown to be linked to an INCREASED RATE OF DISEASE ● Examples - age, diet high in salt or saturated fat, smoking, lack of exercise, genes
33
REQUIRED PRACTICAL 5: Dissection of animal or plant gas exchange system or mass transport system or of organ within such a system. Describe precautions that should be followed when performing a dissection
● COVER any CUTS with a waterproof dressing ● When using a SCALPEL, cut AWAY from body onto a HARD surface ● When using a SCALPEL, use a SHARP blade ● When using a SCALPEL, CARRY with blade protected / pointing DOWN ● Wear disposable GLOVES and DISINFECT HANDS / wash with SOAP ● DISINFECT surfaces / equipment ● Safe DISPOSAL - put gloves / paper towels / organ in a separate BAG / BIN to dispose ● If poisonous chemicals / toxins involved, work in a well VENTILATED environment
34
Suggest an ethical consideration when dissecting animals
● Morally wrong to kill animals just for dissection ● So use animals for dissection that have ALREADY BEEN KILLED (humanely) for meat
35
Describe how you could prepare a temporary mount of a piece of plant tissue for observation with an optical microscope
1. Add a drop of WATER to glass slide 2. Obtain a THIN section of specimen and place on slide 3. STAIN (eg. with iodine / potassium iodide to view starch) 4. Lower COVERSLIP at ANGLE using MOUNTED NEEDLE without trapping air bubbles
36
What are the rules of scientific drawing?
-Look SIMILAR to specimen / image - draw parts to the same SCALE / relative size -NO SKETCHING - only CLEAR, CONTINOUS lines -NO SHADING / hatching -Include a MAGNIFICATION SCALE (eg. x 400) -LABEL with straight, UNCROSSED LINES