Topic 4: Natural Selection and Genetic Modification - PAPER ONE Flashcards

(45 cards)

1
Q

Explain how bacteria can help prove Natural selection

A
  • Bacteria can develop random mutations creating new alleles which can change characteristics. For example, a bacteria could become resistant to a particular antibiotic
  • When exposed to the antibiotic, the resistant bacteria are more likely to survive than the non-resistant bacteria
  • So, it will live longer and reproduce
  • The allele for antibiotic resistance is therefore passed on to offspring and becomes more common in the population
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2
Q

How did Charles Darwin come up with his theory?

A
  • Spent 5 years on a voyage around the world studying plants and animals on a ship HMS Beagle
  • Noticed a variation in members of same species. Those with characteristics most suited to the environment were most likely to survived.
  • Also noticed characteristics could be passed on to offspring
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3
Q

How did Wallace contribute to the theory of evolution?

A
  • Wallace came up with the idea at the same time as Darwin (independently)
  • he observed lots of evidence to help support the theory of evolution by natural selection and published his paper at the same time as Darwin
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4
Q

Which fossils provided evidence of Human evolution?

A

Ardi, Lucy, Turkana boy

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5
Q

What features of ‘Ardi’ provide evidence for evolution?

A

Fossil Hominid 4.4 million year old
- Feet structure suggests she climbed trees, had an ape-like big toe to grasp branches
- Long arms, short legs (more ape like than human)
- Brain size same as a chimpanzee’s
- Leg structure suggests she walked upright. hand bone structure suggests she didn’t use her hands to help walk (like apes do)

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6
Q

What features of ‘Lucy’ provide evidence for evolution?

A

Fossil Hominid 3.2 million years old
- Arched feet more adapted to walking than climbing, no ape-like big toe
- Size of arms and legs between apes and humans
- Brain slightly larger than Ardi’s, still similar in size to chimp’s brain
- Structure of leg bones and feet suggest she walked upright, more efficiently than Ardi

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7
Q

What species are Ardi, Lucy and Turkana Boy from?

A

Write all in italics:
Ardi - Ardipithecus ramidus
Lucy - Australopithecus afarensis
Turkana Boy - Homo erectus

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8
Q

What did Richard Leakey discover?

A

Turkana Boy 1.6 million years old
- Short arms, long legs, more like a human than an ape
- Brain size much larger than lucy’s (similar to human size)
- Structure of legs and feet suggest he was even better adapted to walking upright than Lucy

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9
Q

How does the development of stone tools help to prove evolution?

A

Stone tools gradually became more complex. This means that the brain of the different Homo species were getting larger.

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10
Q

How can scientists identify how old tools/fossils are?

A

1) Structural features: Less complex tools are likely to be older than more complex tools.
2) Stratigraphy (study of rock layers): Tools/fossils found in deeper rock layers are usually older as older rock layers are usually found below younger layers
3) Stone tools are found with carbon-containing material. Carbon-14 dating can be used to date the material

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11
Q

How does the Pentadactyl Limb provide evidence for evolution?

A
  • It’s a limb with 5 digits
  • In each species the pentadactyl limb has a similar bone structure for a different function e.g. human hands and bat wings
  • Similarity in bone structure suggests that species with a pentadactyl limb evolved from a common ancestor
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12
Q

Describe the five kingdom classification system

A

Kingdoms (Animals, plants, fungi, prokaryote, protists) are subdivided into smaller groups…
Phylum, class, order, family, genus, species

KING PHILIP CAME OVER FOR GOOD SOUP

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13
Q

Why do we now use three domains rather than the five kingdoms classification method?

A

Technology has developed (more knowledge due to better microscopes - say this in exam):
- Woese found, using RNA SEQUENCING, that some members of the Prokaryote kingdom were not closely related
- DOMAIN THEORY BASED ON GENETICS USING GENETIC ANALYSIS

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14
Q

What does the three domain method of classification propose?

A
  • He proposed that prokaryote kingdom should be split into Archaea and Bacteria
  • 3 domains were created: Eukarya, Archaea, bacteria
  • These domains are then subdivided into smaller groups used in the 5 kingdom system
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15
Q

What do each of the 3 domains contain?

A

Archaea: Organisms look similar to bacteria but have differences in their DNA and RNA sequences. Found in extreme places (hot springs)
Bacteria: Contains true bacteria
Eukarya: Contains broad range of organisms - fungi, plants, animals and protists

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16
Q

What’s selective breeding?

A

When humans artificially select plants or animals that are going to breed so that the genes for particular characteristics remain in the population

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17
Q

Explain how selective breeding works.

A

1) Select which characteristics you’re after from existing stock and breed them with each other
2) Select the best offspring and breed them together
4) Continue for several generations and the trait gets stronger and stronger
5) Eventually all the offspring will have the characteristic

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18
Q

Explain 2 uses for selective breeding

A
  • Farmers breed together cows and bulls that are larger so they produce more meat. They can continue to do this for several generations so that the offspring will have very high meat yields.
  • Rats have been bred with a strong or weak preference for alcohol so researchers can compare the effects of alcohol on their behaviour and brains to investigate the reasons behind alcoholism.
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19
Q

What are some disadvantages to selective breeding?

A
  • Reduces gene pool (animals/plants closely related - inbreeding)
  • Inbreeding can cause health problems as there’s more chance of organisms inheriting genetic defects when gene pool is limited e.g. heart disease in boxer dogs
  • If a new disease appears many might get killed as there is little variation in the population so there’s less chance of resistance alleles being present
20
Q

What’s tissue culture?

A
  • Involves growing cells on an artificial growth medium
  • Plants produced via tissue culture are clones (genetically identical). So, you can use it to created lines of clones with the same beneficial features (e.g. tasty fruit)
21
Q

Explain the process of growing plants using tissue culture.

A

1) Chose plant to clone based on its characteristics
2) Remove several small pieces of tissue from the parent plant (best results from root or shoot tips)
3) Grow tissue in a growth medium containing nutrients and growth hormones under aseptic conditions to prevent growth of microbes
4) As the tissues produce roots and shoots they can be moved to potting compost to carry on growing

22
Q

How tissue culture used for medical research?

A

You can investigate the effects of a substance or environmental change on the cells of a single tissue without complications from other processes in the whole organism

23
Q

Explain how the tissue culture for animal cells is carried out to be useful for medical research.

A

1) Extract a sample of the tissue you want to study e.g. from the pancreas of an animal
2) Cells in sample are separated from each other using enzymes
3) They are then placed in a culture vessel and bathed in a growth medium containing the nutrients that they need so that they can grow and multiply
4) After several months of cell division, they can be split up again and placed into separate vessels to encourage further growth
5) Once the tissue culture has been grown, it can be stored for future use

24
Q

What’s Genetic Engineering?

A

A process which involves modifying the genome of an organism to introduce desirable characteristics

25
Explain the role of restriction and ligase enzymes
In genetic engineering... - Restriction enzymes recognise specific sequences of DNA and cut the DNA at the points - Ligase enzymes are used to join the 2 pieces of DNA together at their sticky ends - Two different bits of DNA stuck together are known as recombinant DNA
26
Explain the role of vectors in genetic engineering
A vector is something used to transfer DNA into a cell, there are two types: 1) Plasmids are small, circular molecules of DNA that can be transferred between bacteria 2) Viruses insert DNA into the organisms they infect
27
Explain how Genetic Engineering works
1) DNA you want to insert is cut out with restriction enzyme. Vector DNA is then cut open using the same restriction enzyme 2) The vector DNA and DNA you are inserting are left with sticky ends. They are mixed together with ligase enzymes. 3) Ligases join pieces of DNA together to make Recombinant DNA 4) The recombinant DNA (vector containing new DNA) is inserted into other cells e.g. bacteria
28
Explain the pros and cons of using genetic engineering to improve crop yields
Pro: GM crops can increase food production e.g. modified to grow better in drought conditions to improve crop yields/modified to become resistant to diseases Cons: - Many people go hungry because they can't afford food, not because there isn't enough - fears that countries may become dependent on companies who sell genetically modified seeds - Sometimes poor soil is why crops fail, so even GM crops won't survive
29
Explain the pros and cons of using fertilisers to keep up with the growing population
Pro: - Contain minerals essential for plant growth e.g. nitrates and phosphates Con: - Excess fertilisers can run into the rivers and lakes causing eutrophication
30
Explain the pros and cons of using biological control to keep up with the growing population
Biological control methods use other organisms to reduce pest numbers... Pro: - Can have longer-lasting effects than chemical pesticides and is less harmful to wildlife Con: - introducing new organisms can cause problems e.g. cane toads are now a pest in Australia because they poison native species that eat them
31
Species Homo habilis became extinct shortly after Homo Erectus inhabited the earth. Suggest an explanation for the extinction of Homo habilis.
- Likely to have been out competed by homo erectus - For resources essential to survival
32
Describe how stone tools were made.
By hitting them with another rock
33
Describe how the thinner beaks of bird species is a result of evolution.
- There was a variation in beak shape (1) - Thinner beaks are more suited to catching food in the species' environment (1) - Therefore, there birds with thinner beaks outcompeted/obtained more food than birds with thicker beaks of the island (1) - So more birds with thinner beaks survived and passed on their alleles for thinner beaks (1)
34
Explain the pros and cons of using genetic engineering for crop insect resistance.
Pros: - Makes them resistant to pests - This improves crop yields and profits - Reduces need for chemical pesticides - Bacterium used (Bacillus thuringiensis) produces a toxin that kills insect larvae harmful to crops, but is not harmful to humans/animals/other insects Cons: - Long term effect of exposure to Bt crops are not yet known - There's a chance that the insects that feed on the crops will develop resistance as they are constantly exposed to the toxin (so they will no longer be killed by it) - This reduces biodiversity in the area - plants are initially more expensive
35
Describe the method the scientist could use to grow plants from these seeds that are tolerant to low levels of acid in the soil.
- Prepare the soil with a low level of acid - Put seeds in the soil to grow
36
Explain mitochondrial DNA is used as evidence for evolution rather than nuclear DNA.
higher abundance (1) less susceptible to decay (1) (easier to extract)
37
exam tip: for evolution part, in 2 markers mention 2 aspects e.g. a change in stone tools AND change in body structure are evidence for human evolution
:)
38
Explain why the fossil record is incomplete.
* some fossils are yet to be found (1) * fossils may be damaged (1) * conditions not correct for fossil formation (1)
39
Suggest why such a high percentage of oxygen in the atmosphere was needed for large land animals to evolve
large organisms {more complex/carry out greater number of functions / more cells} * for (more aerobic) respiration * for (more) energy
40
Describe what is meant by the term speciation.
The formation of a new species due to geographical isolation/a change of habitat
41
Describe the variation of a graph when values are not increasing/decreasing in order
Discontinuous
42
Suggest how a named product from genetically modified (GM) bacteria can benefit humans
Can produce medicines to cure diseases
43
Describe the stages used in the laboratory to clone animals from a genetically engineered cell
diploid nucleus is removed from the genetically engineered cell  making a lone nucleus  a donor egg has its nucleus is removed  the diploid nucleus from the GE cell is inserted into the enucleated egg cell  division of the nucleus is stimulated  by electric shock/chemicals  cell divides by mitosis  cells put into surrogate mother  cells divide further and differentiates to form an embryo  Animal born
44
Suggest the advantages of cloning mammals, such as dogs, for use in medical research into human diseases
- the clones will all be genetically identical (1) - so test results will be similar / not affect by genes (1) - the clones could be GE to have specific human diseases /(dogs have) similar diseases / disorders to humans (1) - dogs and humans are mammals / have similar anatomy / physiology / DNA
45
for GM exam questions, what does italics e.g. 'Describe how -Agrobacterium tumefaciens- can be used to create transgenic plants.' mean??????
Means it is the vector