Topic 8: The Control of Gene Expression

Question 1

Any DNA in the sample is hydrolyzed by enzymes before in sample is added to the reaction mixture.
Example why. (2 marks)

Answer 1

1. To remove any DNA present
2. As this DNA would be amplified/replicated

Question 2

Scientists have used the RT-PCR method to detect the presence of different RNA viruses in patients suffering from respiratory diseases.
The scientists produced a variety of primers for this procedure.
Explain why. (2 marks)

Answer 2

1. Base sequences differ
2. Different complementary primers required