topic three - enzymes - mr hedditch Flashcards
define an enzyme?
enzymes are biological catalysts. They speed up
chemical reactions without being used up. They are globular proteins with a specific tertiary structure
what are the two types of metabolic reactions?
breaking large molecules into smaller molecules (catabolic)
building smaller molecules up into larger molecules (anabolic)
what’s an enzyme made up of and what structure does it have?
enzymes are proteins with a tertiary structure
define activation energy?
the level of energy required to enable a reaction to
take place
enzymes reduce the amount of energy required to allow a reaction to take place (means that reactions can proceed at lower temperatures)
what would happen to the chemical reactions if there were no enzymes?
many chemical reactions would proceed too slowly to sustain life
define intracellular?
describes an enzyme that remains active only within the cell in which it is formed
define extracellular?
describes an enzyme (such as a digestive enzyme) that functions outside the cell from which it originates
what does catalyse break down?
it catalyses the breakdown of hydrogen peroxide into
water and oxygen :
H2O2 H2O + O2
two examples of extracellular enzymes?
amylase & trypsin
where is amylase produced and what does it do to your mouth?
salivary amylase is produced in the salivary glands and hydrolyses starch to maltose in the mouth
the pancreas also produces amylase, which acts in the small intestine
where is trypsin made and what does it breakdown?
Made in the pancreas and acts
made in the small intestine
breaks peptide bonds and hydrolyses proteins into
smaller polypeptides, ready for other enzymes to break the polypeptides into amino acid
what type of protein is enzyme?
globular proteins
what are the medicinal drugs reduce enzyme activity?
enzyme inhibitors
what are the two different models of enzyme action?
the lock-and-key model and induced-fit model
state the stages of the lock-and-key model?
enzymes are specific to particular substrates
the active site has a shape complementary to the shape of the substrate
the substrate binds to the enzyme to form an enzyme substrate complex
the products are released (no longer fit
the active site)
state the stages of induced-fit model?
shape of the active site does not match the shape of the substrate exactly
active site moulds itself around the substrate
a close fit is needed before the reaction can take place
an enzyme-product complex forms
the products are released (no longer fit the active site)
the enzyme reverts to its original shape