transcription

Question 1

Ribose differ from deoxyribose

Answer 1

addl -OH

Question 2

Uracil differs from Thymine

Answer 2

absence of a -CH3 group

Question 3

All RNA is made by this

Answer 3

DNA transcription

Question 4

mRNA codes in this direction

Answer 4

5’ -to- 3’

Question 5

coding from gene to protein differ in euk and pro, which is more complicated?

Answer 5

Eukaryotes

Question 6

Number of proteins coded in bacterial mRNA, euk mRNA

Answer 6

several, one

Question 7

Main enzyme of transcription

Answer 7

RNA polymerases

Question 8

Start of a chain requires a primer in DNA or RNA

Answer 8

DNA

Question 9

Moves along DNA and adds nucleotides to RNA chain

Answer 9

RNA polymerase

Question 10

form of nucleotide in RNA

Answer 10

ribonucleoside triphosphates

Question 11

regulatory sequence that controls binding of RNA polymerase to DNA and identifies start site

Answer 11

promototer

Question 12

frequency of transcriptions controlled by the regulatory sequences

Answer 12

cis

Question 13

proteins that assist in binding and stability of RNA polymerase

Answer 13

trans

Question 14

bacteria have this many RNA polymerase

Answer 14

one

Question 15

protein that binds the core enzymes

Answer 15

sigma

Question 16

Name the 3 eukaryote RNA polymerases

Answer 16

Pol I, Pol II, Pol III

Question 17

Pol I produces this RNA

Answer 17

rRNA

Question 18

Pol II produces this RNA

Answer 18

mRNA, miRNA, lincRNA

Question 19

Pol III produces this RNA

Answer 19

tRNA and 5SrRNA

Question 20

binding of these cause the DNA strands to unwind

Answer 20

RNA Pol and sigma factor

Question 21

binding of a protein is called this

Answer 21

rho

Question 22

core polymerase plus sigma factor in bacteria

Answer 22

holoenzyme

Question 23

processing required for eukaryote mRNA

Answer 23

addition of 5’ cap, spicing of non-coding introns, addition of poly-A tail

Question 24

eukaryotic require this number if protein subunits for transcription to occur

Answer 24

100

Question 25

DNA sequence bound by transcription factors

Answer 25

TATA box

Question 26

Function of RNA polymeraser II

Answer 26

begin elongation phase of transcription

Question 27

unique to eukaryotes, these proteins are required for transcriptioin

Answer 27

transcriptional activator proteins

Question 28

Transcription factors

Answer 28

TFIIA, TFIIB

Question 29

ensures that exons are accurately spliced

Answer 29

spliceosome

Question 30

mRNA splicing occurs when this nucleotide attacks the splice site

Answer 30

adenine

Question 31

fate of spliced intron

Answer 31

degradation

Question 32

spliceosome is made of assembly of these

Answer 32

snRNPs

Question 33

mRNA leaves nuclear pore via this type of transport

Answer 33

active transport

Question 34

tRNA includes binding sites for these 2 items

Answer 34

anticodon of mRNA and binding site for encodd amino a.

Question 35

Binds TATA box to strand

Answer 35

TATA binding protein (TBP)

Question 36

enviromental toxins damage these proteins and can cause disease

Answer 36

inhibition of RNA polymerase

Question 37

S value for ribosomes

Answer 37

rate of sedimentation = size

Question 38

exon 1 to be spliced (prior to)

Answer 38

GU

Question 39

exon 2 to be spliced (after)

Answer 39

AG

Question 40

ribsomes (euk)

Answer 40

45s precursors -->18s-->40S and 5.8S, 28S -->60S. 40S, 60S --> 80S

Question 41

regulatory sites that are at a distance from the promoter

Answer 41

enhancers

Question 42

effect of activation domain

Answer 42

accelerates transcription

Question 43

IRE

Answer 43

iron response element

Question 44

UTR

Answer 44

untranslated region

Question 45

elf-2 phosphorylation controls this

Answer 45

protein synthesis rates by tying up elF-2B