Transcription I

Question 1

Promoter contains

Answer 1

• consensus DNA sequence TATA
• TFIIB recognition element (BRE)
• initiator sequences (INR)

Question 2

Transcription Factor Binding at Promoter

Answer 2

TFIID binds core promoter region
TFIIA and TFIIB stabilize TFIID binding
TFIIB recruit RNAPII
TFIIF binds RNAPII
TFIIE and TFIIH recruited
– melt, unwind promoter = activate RNAPII

Question 3

Chromatin Organization

Answer 3

• non-randomly distributed in nucleus
• cell type specific
• LADs bind to LAP = repress gene expression

Question 4

Co-Linear Expression of Globulins

Answer 4

transcribed at same area = easier to control

Question 5

Reporter Gene Qualities

Answer 5

• easily detectable
• not normally expressed in cell
• not quickly degraded
• no subject to post-transcription/translation regulation

Question 6

Promoter-Reporter Assays

Answer 6

Luciferase
- emission of light, stable, quantifiable

LacZ
- express β-galactosidase, stable, detectable, in vivo