Trimming

Question 1

The process by which processed tissue is trimmed and cut into uniformly thin slices

Answer 1

MICROTOMY

Question 2

Three parts of Microtome

Answer 2

Block holder (chuck)
Knife carrier and knife
Pawl,ratchet feed wheel and adjustment screws

Question 3

For cutting serial sections of large blocks of paraffin embedded tissues

Answer 3

ROCKING MICROTOME

Question 4

Invented by Minot in 1885-86
Used to cut paraffin embedded tissues

Answer 4

ROTARY MICROTOME

Question 5

The knife and the block holder are brought together by upward and vertical motions
It is heavier and more stable than the rocking microtome

Answer 5

ROTARY MICROTOME

Question 6

Invented by Adams in 1789

Answer 6

SLIDING MICROTOME

Question 7

For cutting of celloidin-embedded tissues
Base sledge microtome

Answer 7

SLIDING MICROTOME

Question 8

Invented by Queckett in 1848

Answer 8

FREEZING MICROTOME

Question 9

A refrigerated apparatus used in fresh tissue microtomy

Answer 9

Cryostat

Question 10

Used for cutting unembedded or gelatin-embedded blocks frozen in liquefied carbon dioxide, liquefied nitrogen,Freon and other refrigerants

Answer 10

FREEZING MICROTOME

Question 11

Used for cutting tissue sections of 0.5 micra for electron microscopy

Answer 11

ULTRATHIN MICROTOME

Question 12

Knives For electron microscopy

Answer 12

Glass knives

Question 13

Knives For electron microscopy

Answer 13

Diamond knives

Question 14

One side of the knife is flat while the other is concave

Answer 14

Plane-concave knife (25mm.in length)

Question 15

Both sides are concave

Answer 15

Biconcave knife (120 mm. in length)

Question 16

Both sides are straight

Answer 16

Plane-wedge knife (100mm in length)

Question 17

The angle formed between the cutting edges
27°-32°

Answer 17

Bevel angle

Question 18

The angle between the block face and lower facet of knife
10°-15°

Answer 18

Clearance angle

Question 19

Removal of gross nicks on the knife edge (coarse honing)
To remove blemishes

Answer 19

HONING

Question 20

Types of Honing

Answer 20

Belgian Yellow
Arkansas
Finecarborundum

Question 21

Gives the best result

Answer 21

Belgian Yellow

Question 22

10-20 strokes hone

Answer 22

plane-wedge knife

Question 23

20-40 strokes hone

Answer 23

biconcave knife

Question 24

Facilitates honing & removes metal cutting
Light oil
Dilute paraffin solution
Xylene

Answer 24

Lubricant

Question 25

Process whereby the "burr" formed during honing is removed and the cutting edge of the knife is polished

Answer 25

Stropping

Question 26

80-120 strokes on each side strope

Answer 26

Biconcave knife