Types of Microscope Preparations and Types of Microtomes Flashcards

(29 cards)

1
Q

Size of a microscope slide

A

75 x 26 mm (3 x 1 inch)

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2
Q

Used to hold objects under a microscope

A

Microscope Slide

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3
Q

The type of microscope slide that has round indentations/depressions in the slide

A

Concave Style

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4
Q

The type of microscope slide that is flat and smooth on the surface

A

Flat Style

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5
Q

Microscope slide that can be stored for a long time

A

Permanent Slide

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6
Q

Few trapped air bubbles, less chance of sample loss

A

Permanent Slide

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7
Q

Two types of temporary slides

A

Wet mount and Dry mount

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8
Q

Made for short time observations

A

Temporary Slides

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9
Q

Usually uses liquids, thus is discarded after use

A

Temporary Slide

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10
Q

Uses liquids like water, glycerin, and oil immersion as a medium

A

Wet mount slides

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11
Q

A liquid that has preserving characteristics

A

Glycerine

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12
Q

Does not need a mounting medium

A

Dry Mount slide

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13
Q

Places a cover slip

A

Dry mount slides

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14
Q

It may lower low quality images

A

Dry mount slide

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15
Q

There is heat involved

A

Heat Fixed Slide

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16
Q

Immersion oil is placed directly on the slide and the 100x oil immersion objective is used to observe the bacteria

A

Heat Fixed Microscope Slide

17
Q

Smears, its angle determines the length of the smear; a steeper angle corrects a shorter smear

18
Q

Designed for soft samples, literally squishes the specimen

A

Squash slides

19
Q

Stains the slides, samples such us iodine, methylene blue, and crystal violent

A

Staining slides

20
Q

Use for cutting sections at a specific thickness by sliding a block i to a cutting tool

21
Q

Where the tissue is held in position

22
Q

For the actual cutting and experience

A

Knife and carrier and knife

23
Q

To line up the tissue block in the proper position with the knife

A

Pawl, Ratchet feed wheel and adjustment screws

24
Q

Cutting serial sections of large blocks of paraffin embedded tissues

A

Rocking Microtome

25
For cutting paraffin embedded section
Rotary Microtome
26
For cutting celloidin embedded section
Sliding Microtome
27
For cutting unembedded frozen sections
Freezing Microtome
28
For cutting frozen sections
Cryostat or Cold Microtome
29
For cutting sections for electron microscopy
Ultrathin Microscope