Unit 2 - metabolism and survival Flashcards
What makes up a membrane
Bilayer of phospholipids with proteins embedded in it
What term is used to describe the structure of a membrane
Fluid mosaic model
What type of membrane is selectively permeable
Plasma membrane
Why does a membrane form compartments
To keep metabolic processes in specific regions/ to keep enzymes apart
Why is compartmentalisation import in cells like mitochondria
The folds increases surface area which makes reactions faster, ie more ATP can be produced
What are the 4 membrane protein functions
Enzymes, channel (pore) proteins, carrier (pump) proteins and structural support
What allows specific molecules to pass through the membrane
Channel (pore) proteins
What is the purpose of carrier proteins
Binds to specific molecule temporarily to let them cross the membrane
What is a catabolic pathway
Breakdown of complex molecules to simpler ones (releases energy)
What is an anabolic pathway
Biosynthesis of complex molecules from simpler building blocks
Give the definition of a metabolic pathway
Series of chemical reactions that follow one after another
What controls metabolic pathways
Enzymes
Give an example of a reversible metabolic pathway
Anaerobic respiration in mammals
What can interrupt metabolic pathways
Vemons, toxins and poisons
What is activation energy?
Energy required to initially break bonds for a reaction to happen
What effect does an enzyme have on the activation energy
Lowers activation energy
What is the enzyme function described as
An induced fit
When a substrate binds to the active site does it have a low or high affinity for the active site
High affinity
When substrate does the substrate have a low affinity for the active site
When it moves away from the active site
Is the active site a perfect fit for the substrate?
No it is a near perfect fit and then adapts to fit perfectly around the substrate
What is a co-factor
Molecules needed to make the enzyme more efficient
What term is given to enzymes that work in groups
Multi enzyme complex
How can cells control their enzyme activity
- Controlling number of enzymes
- changing enzyme shape
- keeping reactions in compartments
What is an enzyme inhibitor
Reduces rate of enzyme activity by interfering with enzyme
What is competitive inhibition
Inhibitor has similar shape to the substrate molecule, fits into active site but remains unreacted, decreases rate
Is competitive inhibition temporary or permanent
Temporary - effects reduced by increasing substrate concentration
How does a non-competitive inhibitor work
Inhibitor binds to enzyme but not to active site, shape of active site altered, substrate no longer fits
What is feedback inhibition
Rate controlled by end product build up
Where does the energy required to build up ATP from ADP come from
The breakdown of glucose
What are the purposes of ATP
- provide energy for cellular processes
- needed for phosphorylation reactions
What does ATP stand for
Adenosine triphosphate
Where does glycolysis take place
In the cytoplasm
What is the net gain of ATP from glycolysis
2 ATP
What enzyme removes the H ions and electrons
Enzyme dehydrogenase
What happens in the energy investment stage of glycolysis
Glucose is broken down to an intermediate which is broken down to another intermediate. 2ATP are required for phosphorylation to the intermediate
What happens in the energy pay off stage of glycolysis
The intermediate is broken down into pyruvate. 4ADP + Pi is build up to 4ATP. The enzyme dehydrogenase removes the H ions ad electrons passing them onto the coenzyme NAD which becomes NADH
Where does the critic acid cycle take place
In the matrix of the mitochondria
What does pyruvate break down into during the citric acid cycle
Acetyl
What bonds with acetyl to become acetyl coenzyme A
Co enzyme A
What two compounds join to make citrate in the citric acid cycle
Acetyl coenzyme A and oxaloacetate
What is released during the citric acid cycle
Carbon dioxide
FAD becomes what during the citric acid cycle
FADH2