Unit 2 Test Flashcards
What are the parts of the nucleotide building block
The nitrogenous base, a sugar molecule (deoxyribose), and a phosphate.
Base pairing-pyrimidines and purines
Chargaff’s rule: A-T and C-G, Adenine and Guanine are purines, Thymine and Cytosine are pyrimidines.
Parts of the double helix
Deoxyribose and phosphate alternating (in the actual helix).
The Polymerase role
Builds the new DNA strand (by reading the parent strand and matching nucleotides).
Ligase role
Glues okazaki fragments on the lagging strand together.
Helicase role
Unwinds and unzips the double stranded DNA via breaking the hydrogen bonds between bases.
Unzipping is when
helicase breaks the hydrogen bonds
The Replication fork is
the shape the unzipped DNA makes
Copying is
polymerase recognizes the primer in which primase prepares and uses free nucleotides to build the new strand via parent code.
Base pairing
adenine (A), cytosine (C), guanine (G), thymine (T)… A = T and C = G
How many bonds do Cytosine and guanine have?
3 hydrogen bonds
How many bonds do Adenine and thymine have?
2 hydrogen bonds
What direction is the leading strand made in?
(3’ to 5’) as the moving fork
What direction is the lagging strand made in?
(5’ to 3’) in short Okazaki fragments.
Okazaki Fragments
Short synthesized DNA sequences that get put together to make the lagging strand of DNA.
semi conservative
Two copies of the original DNA molecule are produced. Each copy has one original strand and one newly-synthesized strand, hence semi-conservative.
Some facts about DNA
Its sugar is deoxyribose, it has double-stranded molecules, its stores and transfers genetic information, and it has thymine.
Some facts about RNA
Its sugar is ribose, it has single-stranded molecules, it codes for amino acids and acts as a messenger between DNA and ribosomes to make proteins, and it has uracil.
Parts of the nucleotide building block
Sugar (ribose), base and phosphate.
mRNA
(messenger): encodes amino acid sequence of a polypeptide.
tRNA
(transfer): brings amino acids to ribosomes during translation.
rRNA
(ribosomal): with ribosomal proteins, makes up the ribosomes.
Transcription
the synthesis of RNA from a DNA template where the code in the DNA is converted into a complementary RNA code.
Translation
the synthesis of a protein from an mRNA template where the code in the mRNA is converted into an amino acid sequence in a protein.