Unit 2 Test Flashcards

1
Q

What are the parts of the nucleotide building block

A

The nitrogenous base, a sugar molecule (deoxyribose), and a phosphate.

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2
Q

Base pairing-pyrimidines and purines

A

Chargaff’s rule: A-T and C-G, Adenine and Guanine are purines, Thymine and Cytosine are pyrimidines.

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3
Q

Parts of the double helix

A

Deoxyribose and phosphate alternating (in the actual helix).

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4
Q

The Polymerase role

A

Builds the new DNA strand (by reading the parent strand and matching nucleotides).

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5
Q

Ligase role

A

Glues okazaki fragments on the lagging strand together.

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6
Q

Helicase role

A

Unwinds and unzips the double stranded DNA via breaking the hydrogen bonds between bases.

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7
Q

Unzipping is when

A

helicase breaks the hydrogen bonds

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8
Q

The Replication fork is

A

the shape the unzipped DNA makes

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9
Q

Copying is

A

polymerase recognizes the primer in which primase prepares and uses free nucleotides to build the new strand via parent code.

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10
Q

Base pairing

A

adenine (A), cytosine (C), guanine (G), thymine (T)… A = T and C = G

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11
Q

How many bonds do Cytosine and guanine have?

A

3 hydrogen bonds

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12
Q

How many bonds do Adenine and thymine have?

A

2 hydrogen bonds

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13
Q

What direction is the leading strand made in?

A

(3’ to 5’) as the moving fork

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14
Q

What direction is the lagging strand made in?

A

(5’ to 3’) in short Okazaki fragments.

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15
Q

Okazaki Fragments

A

Short synthesized DNA sequences that get put together to make the lagging strand of DNA.

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16
Q

semi conservative

A

Two copies of the original DNA molecule are produced. Each copy has one original strand and one newly-synthesized strand, hence semi-conservative.

17
Q

Some facts about DNA

A

Its sugar is deoxyribose, it has double-stranded molecules, its stores and transfers genetic information, and it has thymine.

18
Q

Some facts about RNA

A

Its sugar is ribose, it has single-stranded molecules, it codes for amino acids and acts as a messenger between DNA and ribosomes to make proteins, and it has uracil.

19
Q

Parts of the nucleotide building block

A

Sugar (ribose), base and phosphate.

20
Q

mRNA

A

(messenger): encodes amino acid sequence of a polypeptide.

21
Q

tRNA

A

(transfer): brings amino acids to ribosomes during translation.

22
Q

rRNA

A

(ribosomal): with ribosomal proteins, makes up the ribosomes.

23
Q

Transcription

A

the synthesis of RNA from a DNA template where the code in the DNA is converted into a complementary RNA code.

24
Q

Translation

A

the synthesis of a protein from an mRNA template where the code in the mRNA is converted into an amino acid sequence in a protein.

25
Ribosomes
are where protein synthesis happens
26
RNA polymerase
builds the new DNA strand
27
Codons
are the three-letter “words” in mRNA
28
Anticodons
are the three unpaired bases on tRNA
29
What are some changes in Chromosomal mutations
Duplication: production of an extra part (or an entire) chromosome. Deletion: loss of a part or all of a chromosome. Inversion: reverses the direction of parts of a chromosome. Translocation: one part of a chromosome breaks off and attaches to another chromosome.
30
What are some changes in Gene Mutations
Substitution: a single nucleotide is replaced. May result in a single amino acid change. Insertion: a single nucleotide is inserted into the sequence. All amino acids after the mutation may be changed. Deletion: a nucleotide is deleted. All amino acids after the mutation may be changed.
31
Chromosomal mutations
involve changes in the number or structure of chromosomes
32
Gene mutations
involve changes in one or a few nucleotides are known as point mutations
33
Recombinant DNA
DNA produced by combining DNA from different sources
34
Restriction enzymes
enzyme that cuts DNA at a sequence of nucleotides
35
Plasmid
small, circular piece of DNA in the cytoplasm of many bacteria
36
Cloning (including twins)
Member of a population of genetically identical cells produced from a single cell
37
Transgenic Organisms (GMO’s)
Organisms that contain genes from other organisms
38
Gene therapy
Changing a gene to treat a medical disease/disorder