Unit 3 (pre-test 1) Flashcards
(40 cards)
what are the 4 methods of transport
Explain the Ran-GTP gradient and how it drives nuclear import/export + how it is reestablished
what regulates NPC transport?
What are the 3 types of proteins that bind import receptors during their normal function while they import proteins into the nucleus
Why can small molecules freely diffuse in/out of the nucleus while larger ones can’t
whats the max size for free nucleus diffusion
explain subcellular fractionating and how we do it
how do we prove a signal sequence is necessary and sufficient for localization of a protein to a specific compartment
explain what a sorting signal is and what happens to proteins that don’t have one
explain the difference between a sorting sequence and a sorting patch
is the plasma membrane a small or large fraction of the total membrane in a eukaryotic cell?
how is nuclear import/export regulated
answer relates to the avalibility of the NLS sequence
how does chloroplast transport compare to mitochondrial transport
what are Ran’s other jobs
what do GEFs and GAPs do? What do they stand for?
How do nuclear import receptors get into the nucleus? what do they bind to?
explain the process of nuclear import
how is the GTP/GDP gradient maintained during mitosis?
what is NPC?
what does the nuclear membrane look like? what organelle is it continous with?
what is the nuclear import sequence and where would you find it on a polypeptide?
What is rich in K and R (relating to nuclear transport)
what moves in and out of the nucleus?
what are the 4 evolutionary cell compartment classes
