Unit 4

Question 1

The GasPak anaerobic system will remove _____, and replace it with _____ and _____.

Answer 1

Oxygen, Hydrogen and Carbon Dioxide

Question 2

This oxidase positive, gram negative diplococci is not part of normal human flora and is spread person to person by sexual contact.

Answer 2

Neisseria gonorrhoeae

Question 3

To determine if an organism is definitely an obligate anaerobe what procedure must be performed?

Answer 3

Aerotolerance test

Question 4

This organism may cause lethal meningitis but is commonly carried in the respiratory tract of humans and may be asymptomatic.

Answer 4

Neisseria meningitdis

Question 5

The candle jar will be useful when culturing organisms requiring

Answer 5

increased carbon dioxide

Question 6

This slender anaerobic gram negative rod produces a fluorescent chartreuse color and is sensitive to colistin and kanamycin

Answer 6

Fusobacterium nucleatum

Question 7

All of the following medium are examples of PRAS media except:
Anaerobic blood agar
LKV agar
BBE agar
Modified Thayer Martin

Answer 7

Modified Thayer Martin

Question 8

Among H. influenzae strains, group designations for serotyping are based on:

Answer 8

capsular characteristics

Question 9

A colony was taken from growth on a chocolate plate from a respiratory specimen. The gram stain is gram negative diplococci. What is the next test that should be performed?

Answer 9

Oxidase

Question 10

Which atmospheric condition is optimum for the cultivation of Campylobacter jejuni?

Answer 10

Microaerophilic

Question 11

Proprionibacterium acnes will appear on gram stain as:

Answer 11

small club shaped gram pos rods

Question 12

All of these are indicative of the presence of anaerobes, except:
Goul odor
Growth at the surface of liquid (thioglycollate) media
Gas
Positive gram stain with no aerobic growth

Answer 12

Growth at the surface of liquid (thioglycollate) media

Question 13

Indicate whether the following clinical specimens are suitable or unsuitable for anaerobic culture:
Bile

Answer 13

Suitable

Question 14

Indicate whether the following clinical specimens are suitable or unsuitable for anaerobic culture:
Throat swab

Answer 14

Unsuitable

Question 15

Indicate whether the following clinical specimens are suitable or unsuitable for anaerobic culture:
Coughed (expectorated) sputum

Answer 15

Unsuitable

Question 16

Indicate whether the following clinical specimens are suitable or unsuitable for anaerobic culture:
Pleural fluid

Answer 16

Suitable

Question 17

Indicate whether the following clinical specimens are suitable or unsuitable for anaerobic culture:
Endometrium

Answer 17

Suitable

Question 18

Indicate whether the following clinical specimens are suitable or unsuitable for anaerobic culture:
Blood

Answer 18

Suitable

Question 19

Indicate whether the following clinical specimens are suitable or unsuitable for anaerobic culture:
Feces

Answer 19

Unsuitable

Question 20

Indicate whether the following clinical specimens are suitable or unsuitable for anaerobic culture:
Aspirated bladder urine

Answer 20

Suitable

Question 21

Match the organism with the appropriate term, disease or selective media.
CCFA agar

Answer 21

Clostridium difficile

Question 22

Match the organism with the appropriate term, disease or selective media.
Gas gangrene

Answer 22

Clostridium perfringens

Question 23

Match the organism with the appropriate term, disease or selective media.
Botox

Answer 23

Clostridium botulinum

Question 24

Match the organism with the appropriate term, disease or selective media.
Satellite test

Answer 24

Haemophilus influenzae

Question 25

Match the organism with the appropriate term, disease or selective media. Modified Skirrow agar

Answer 25

Campylobacter jejuni

Question 26

Match the organism with the appropriate term, disease or selective media. BBE agar

Answer 26

Bacteroides fragilis

Question 27

Match the organism with the appropriate term, disease or selective media. Modified Thayer Martin

Answer 27

Neisseria gonorrhoeae

Question 28

Match the organism with the appropriate term, disease or selective media. Bread crumb colonies

Answer 28

Fusobacterium nucleatum

Question 29

Match the organism with the appropriate term, disease or selective media. Molar tooth colonies

Answer 29

Actinomyces sp.

Question 30

True or False? | A direct gram stain of endocervical secretions is diagnostic of the presence or absence of N. gonorrhoeae

Answer 30

False

Question 31

True or False? | Campylobacter jejuni, Moraxella catarrhalis and Neisseria meningitidis are all oxidase positive.

Answer 31

True

Question 32

Vaccination is responsible for the reduced incidence of severe invasive infections caused by:

Answer 32

Haemophilus influenzae

Question 33

The optimum temperature for the primary cultivation of campylobacter jejuni is:

Answer 33

42 degrees C

Question 34

A positive ALA test is useful for the identification of certain Haemophilus spp. by establishing the following:

Answer 34

X factor independence

Question 35

True or False? | All specimens received for anaerobic culture should be plated and incubated for 18-24 hours.

Answer 35

False

Question 36

``` Infection with which of the following organisms is not associated with the ingestion of food products: Haemophilus influenzae Campylobacter jejuni Clostridium botulinum Clostridium perfringens ```

Answer 36

Haemophilus influenzae

Question 37

Performing the CLO test from a biopsy will cause a color change when this organism is detected:

Answer 37

Helicobacter pylori

Question 38

A typical gram stain of Haemophilus influenzae is best described as:

Answer 38

Gram negative coccobacilli

Question 39

Which plate should be used for the primary cultivation of Haemophilus influenzae?

Answer 39

Chocolate agar

Question 40

``` Which of these anaerobes is part of the normal flora of teh skin and is rarely implicated in infections? Bacteroides fragilis Clostridium perfringens Proprionibacterium sp. Clostridium botulinum ```

Answer 40

Proprionibacterium sp.

Question 41

The development of Guillan-Barre syndrome is associated with infection with which organism?

Answer 41

Campylobacter jejuni

Question 42

This factor, present in several bacteria and yeast, is responsible for satellite growth of Haemophilus sp. around colonies of Staphylococcus species on sheep blood agar.

Answer 42

NAD

Question 43

This organism produces a powerful preformed toxin which may be ingested with food and lead to respiratory paralysis.

Answer 43

Clostridium botulinum

Question 44

``` Which growth requirement is not appropriate for the fultivation of Haemophilus influenzae: 5-10% CO2 Microaerophilic environment X and V factors 35-37 degree C incubation ```

Answer 44

Microaerophilic environment

Question 45

The proper specimen to collect when H. pylori is suspected is:

Answer 45

Gastric biopsy

Question 46

``` Which of the following is a gram positive rod: Haemophilus influenzae Campylobacter jejuni Moraxella catarrhalis Clostridium difficile ```

Answer 46

Clostridium difficile

Question 47

This organism is known to be the major cause of bacterial meningitis worldwide among non-vaccinated children

Answer 47

Haemophilus influenzae

Question 48

When performing the ALA test, the detection of this end product will indicate factor independence.

Answer 48

Porphyrins

Question 49

``` A CSF (cerebrospinal fluid) specimen from a 21 yo college student grew a pure culture of moist grey colonies. The following lab results were observed: Gram stain = gram negative diplococci BAP and Chocolate agar = aerobic growth in CO2 incubator MacConkey and PEA= no growth Oxidase= positive DNase - neg Carbohydrate fermentation = glucose-pos, maltose–pos, sucrose–neg, lactose–neg What is the most likely organism? ```

Answer 49

Neisseria meningiditis

Question 50

A diabetic patient presents to the emergency department with excrutiating foot pain. Upon further exam he is found to have a foul smelling wound at the base of his foot. A piece of tissue is submitted for aerobic and anerobic culture. The culture results are as follows. No growth on Blood, Chocolate and Macconkey agar incubated at 35degrees in ambient air. Growth on PRAS-ANA brucella plate inubated in a jar with a Gaspak Growth in the bottom of a thioglycollate broth. Gram stain=large gram positive (and gram variable) rods, no spores observed PRAS-ANA brucella agar shows double zone of beta hemolysis Special potency disks = Kanamycin-sensitive, Vancomycin-sensitive, Colistin-resistant Which organism is most likely the agent of his infection?

Answer 50

Clostridium perfringens

Question 51

A patient has developed renal symptoms. After a series of tests a liver abscess was found. She had recently undergone surgery of the small bowel. The abscess was aspirated and sent to lab on swabs and in special anaerobic collection tubes. The following culture results were observed. Growth on ANA blood plate and LKV plate after 48 hour anaerobic incubation. (no hemolysis) Subsequent culture of those colonies to an aerobic chocolate plate resulted in no growth. Blackening was observed on BBE agar. Special potency disks: Kanamycin-resistant Vancomycin-resistant Colistin-resistant What is the most likley ID?

Answer 51

Bacteroides fragilis

Question 52

A specimen was isolated from the cerebrospinal fluid of a 4 yr old girl visiting from outside the US. The following results were observed after 48 hr incubation in CO2. Choc = small grey colonies with a mouse-like odor Gram stain= gram negative coccobacilli Blood agar= no growth MacConkey = no growth Satteliting with Staphylococcus = positive ALA= neg The most likely organism is:

Answer 52

Haemophilus influenzae

Question 53

A sputum specimen was obtained from a 79 year old male. He has COPD (chronic obstructive pulmonary disease). The xrays suggest pneumonia. His culture results are as follows: Growth on Chocolate and Blood agar: large opague colonies Macconkey= no growth Gram stain = gram negative diplococci satellite test = negative Oxidase=positive Carbohydrate fermentation: Glucose=neg, Maltose=neg, Sucrose=neg, Lactose=neg Dnase=positive Beta lactamase=negative

Answer 53

Moraxella catarrhalis

Question 54

``` Cultivation of H. ducreyi will require all of the following except: 4-7 day incubation X Factor V Factor high humidity ```

Answer 54

V Factor

Question 55

Actinomyces is usually involved in infections of

Answer 55

Oral/facial area

Question 56

A post surgical drainage from an abdominal wound grew an anaerobic organism on a blood agar plate at 48 hours. It is not aerotolerant. The gram stain showed gram neg bacilli. The following reactions were observed: Nitrate = neg Catalase= negative Growth on BBE = neg (no blackening) Kanamycin = sensitive ,Vancomycin = resistant , Colistin= sensitive The organism will likely be identified as:

Answer 56

Fusobacterium nucleatum

Question 57

The ingestion of honey is not recommended for young children because it may contain the spores of this anaerobic organism.

Answer 57

Clostridium botulinum