Unit 5 Chapters 20, 27-31 The Evolutionary History Of Biodiversity, Biotechnology, Plants, Fungi, Protists, Bacteria, And Archrea Flashcards
(153 cards)
Which of the recombinant DNA drugs is incorrectly matched with its indication (the disease it is prescribed totreat)?
Neupogen (Amgen): Multiple sclerosis (Interferon)
Ian Wilmut
Cloning of Dolly the Sheep
Jennifer Doudna
CRISPR Cas9
W. French Anderson
First Human Gene Therapy
Shinya Yamanaka
induced Pluripotent Stem Cells
Stanley Cohen
Isolation of bacterial plasmids
Kary Mulis
Polymerase chain reaction
Herb Boyer
Isolation of restriction enzymes, Founder of Genentech
James Thompson
Derivation of Human embryonic stem cells
Fred Sanger
Dideoxy DNA sequencing
Craig venter
Whole genome shotgun sequencing
In the polymerase chain reaction (PCR) technique, a heating phase and a cooling phase alternate in cycles. Anoriginal sample of DNA would have to pass through how many total cycles to amplify the DNA ~1 billiontimes? (What about 2 billion times? What about 1 trillion times?)
C. 30
This figure shows the steps in cloning the human insulin gene. What process or enzyme was used for Step 1 ?
C. A restriction enzyme
Gene therapy treatments with new drugs like Kymriah and Yescarta (select all that apply)
B. The first FDA-approved drugs for immunotherapy to treat specific treatments
C. Involves the use of genetically engineered Chimeric Antigen Receptor T-cell to recognize proteins like CD19 on cancer cells
Match the drug with the disease ? Breast Cancer - HER 2+
Herceptin
Non-Hodgkin Lymphoma CD20+
Rituxin
T-cell autoimmune diseases like Rheumatoid arthritis and Psoriasis
Enbrel
RSV
Synagis
Cancer (antiVEGF, anti angiogenesis)
Avastin
PAM
A 3-6 nucleotide DNA sequence immediately downstream of the target site in the genomic DNA to be edited
Cas9
An ending lease that can create double-stranded breaks in DNA
SgRNA
An RNA molecule engineered to be complementary to the target to be edited
Which of these statements is NOT TRUE regarding the procedure for making cloned animals by somaticcell nuclear transfer?
The donor nucleus is from a haploid, quiescent cell
Idaho Gem
First cloned mule