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Microbiology Lab > Unit 6 > Flashcards

Unit 6 Flashcards

1
Q

Which personal items are allowed at the bench?

A. Ipads and Cell Phones
B. Sunglasses and your favorite pen
C. Selfie Sticks
D. Flip flops and your lab notebook
E. None of these are allowed at the bench

A

E. None of these are allowed at the bench

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2
Q

When evaluating a Gram stain of a single Gram(+) species, choose all of the things that would make it a very good stain.

A. The cells are stained all one color.
B. The cells are all purple.
C. The cells are spread evenly and in one layer over the slide.
D. The cells have varying colors
E. The cells are all pink
F. The cells are in clumps

A

A. The cells are stained all one color.
B. The cells are all purple.
C. The cells are spread evenly and in one layer over the slide.

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3
Q

Capsules are often slimy. They allow cells to stick to things and protect cells from white blood cells that might want to eat them. The correct term for “cell eating” is ________________.

A

Phagocytosis

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4
Q

What is the name of the flagella type that has flagella tufted or clustered at the end?

A. Monotrichous
B. Amphitrichous
C. Lophotrichous
D. Peritrichous

A

C. Lophotrichous

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5
Q

A dichotomous key is a tool that asks questions to allow identification of something by process of elimination.

A. True
B. False

A

A. True

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6
Q

The presence of an endospore can contribute to successful colonization of a host and then the host might get ill. This sort of characteristic is called a ___________ factor.

A

Virulence

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7
Q

RIESTIA MEGATERIUM and BACILLUS SUBTILIS are bacillus shaped species that produce multiple small round structures inside that hold nutrients. These structures are called _________ _____.

A

Inclusion body/inclusion bodies

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8
Q

This image shows a cell with ___________ flagella.

A. Monotrichous
B. Amphitrichous
C. Lophotrichous
D. Peritrichous

A

B. Amphitrichous

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9
Q

Choose all the items that are true of Candida albicans

A. C. albicans can reproduce through budding.
B. C. albicans is never pathogenic to humans
C. C. albicans is a bacterial species
D. C. albicans is Eukaryotic
E. C. ALBICANS never has hyphae
F. C. albicans has a nucleus

A

A. C. albicans can reproduce through budding.
D. C. albicans is Eukaryotic
F. C. albicans has a nucleus

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10
Q

The cells in the left photo have ____ endospores.

A. terminal
B. multiple
C. central

A

A. terminal

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11
Q

Inclusion bodies will be helpful to recognize, especially on the final project, so that if you see them you

A. will know you are looking at a staph arrangement
B. will know you are looking at rod shaped cells full of little round inclusion bodies, not coccal shaped cells in a strepto arrangement
C. can impress your friends and neighbors.
D. will know you are looking at a strep arrangement

A

B. will know you are looking at rod shaped cells full of little round inclusion bodies, not coccal shaped cells in a strepto arrangement

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12
Q

A simple stain will help you determine all of the following EXCEPT:

A. Colony size
B. Cell size
C. Cell shape
D. Cell arrangement

A

A. Colony size

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13
Q

The scale bar shown is 10 um in length. What is the length of of a single typical bacillus cell shown?

A. 2 millimeters
B. 0.5 um
C. 2 micrometers
D. 0.5 mm

A

C. 2 micrometers

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14
Q

If you had to make a dichotomous key to separate out these three species of bacteria, which of the following would be an appropriate first question?

A. Does it have endospores?
B. Does it have inclusion bodies?
C. Is it peritrichous?
D. Is it acid fast?

A

A. Does it have endospores?

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15
Q

One of these pictures is C. albicans and the other shows inclusion bodies. Which one is C. albicans?

A

A.

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16
Q

Name the flagellar arrangement seen on this bacterial cell.

A. Monotrichous
B. Amphitrichous
C. Lophotrichous
D. Peritrichous

A

C. Lophotrichous

17
Q

What is the name of the flagella type that only has one flagellum?

A. Monotrichous
B. Amphitrichous
C. Lophotrichous
D. Peritrichous

A

A. Monotrichous

18
Q

This image shows yeast and bacteria. What color is the yeast? Your choices are blue or yellow.

A

Yellow

19
Q

You can tell the difference between inclusion bodies and endospores inside bacterial cells because there can be ______________ inclusion bodies in a cell, but there can only be one endospore.

A

Multiple

20
Q

What is the name of the flagella type that has flagella at both ends of the cell (each side or pole)?

A. Monotrichous
B. Amphitrichous
C. Lophotrichous
D. Peritrichous

A

B. Amphitrichous

21
Q

Virulence ________ include things like flagella that increase the success of pathogens in the host.

A

Factor

22
Q

An endospore is an advantage to a bacterium because:

A. It helps the cell adhere to surfaces
B. It protects the cells from being eaten (phagocytosis).
C. It protects the DNA of the organism under harsh conditions.
D. It is difficult for antibiotics to penetrate the endospore wall

A

C. It protects the DNA of the organism under harsh conditions.

23
Q

Suppose you had to make a dichotomous key for these species. Both of these questions would be fine as the first question.

Does the species have hyphae?
Does the species have flagella?

A. True
B. False

A

A. True

24
Q

What color are Gram(+) cells?

A. Purple
B. Pink

A

A. Purple

25
Q

Name the flagellar arrangement seen on this bacterial cell.

A. Monotrichous
B. Amphitrichous
C. Lophotrichous
D. Peritrichous

A

A. Monotrichous

26
Q

These are red blood cells. Using the scale bar, estimate the size of a typical round cell shown below.

A. 10 um
B. 8 um
C. 6.25 um
D. 0.16 um

A

C. 6.25 um

27
Q

Only one of the species we looked at in this Unit had hyphae and was eukaryotic. That was CANDIDA ALBICANS, a yeast.

A. True
B. False

A

A. True

28
Q

The structure that allows prokaryotic movement.

A

Flagellum

29
Q

A capsule can prevent a species from being phagocytosed by our white blood cells, so capsules are considered virulence factors.

A. True
B. False

A

A. True

30
Q

List the name (chemical or dye) of the primary stain of the acid fast stain.

A

carbolfuchsin, carbol fuchsin

31
Q

This is a dichotomous key, and it would work to ID species A.

A. True
B. False

A

B. False

Reason: It asks yes or no questions , but it regroups species that were split apart and it never singles out individual species.

Microbiology Lab (9 decks)

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