UNIT 6-8 PRACS Flashcards

(58 cards)

1
Q

Counting chamber are seen under what miscroscope lens

A

SCANNING OBJ

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2
Q

The entirety

A

primary squares

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3
Q

Also called secondary corner
squares;

○ When viewing one secondary
square, we then switch to LPO.

A

secondary squares

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4
Q

squares in the middle
portion

A

Tertiary squares

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5
Q

smaller squares in
the middle portion

A

small central squares

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6
Q

THICKER AND SHORTER than WBC

A

RBC THOMA

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7
Q

With graduations: 0.5, 1, 101

A

RBC

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8
Q

LONGER BUT THINNER than RBC

A

WBC

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9
Q

With graduations: 0.5, 1, 11

A

WBC

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10
Q

Has larger bore

A

WBC

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11
Q

1.) Thick cover slip

2.) Suction device

A

ACCESSORY DEVICES

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12
Q

volume of bulb: RBC pipette

A

100 UNITS

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13
Q

volume of bulb of WBC

A

10 units

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14
Q

Bore size for the RBC is smaller, for WBC is larger.

A

TRUE

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15
Q

RBC OVERALL CAPACITY

A

101 UNITS

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16
Q

WBC OVERALL CAPACITY

A

10 UNITS

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17
Q

HOW MANY PARALLEL PLATFORMS SA IMPROVED NEUBAUER?

A

3 parallel platforms

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18
Q

1 primary square is equal to

A

3 x 3mm (9sqmm)

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19
Q

4 corner secondary squares is equal to

A

0.25 x 0.25 mm

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20
Q

central secondary square is equal to

A

0.2 x 0.2 mm

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21
Q

how many tertiary squares for RBC count for IMPROVED NEUBAUER

A

5 of the 25 tertiary squares

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22
Q

how many tertiary squares for WBC count in IMPROVED NEUBAUER

A

16 tertiary squares

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23
Q

how many tertiary squares for RBC count for NEUBAUER

A

5 of the 16 tertiary squares

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24
Q

The Fuchs-Rosenthal counting

grid has an area of?

and a depth of?

A

4 mm x 4mm

depth 0.2mm

25
depth of speirs levy
0.2mm
26
The area is arranged into 2 horizontal rows of 5 squares.
speirs-levy
27
total area of speirs levy
1x1mm (total area of 10sq mm)
28
used in counting the cellular elements of the blood.
HEMACYTOMETER
29
RBC has smaller bore compared to WBC pipette. TRUE OR FALSE?
TRUE
30
MUST BE A HYPOTONIC SOLUTION
TRUE- WBC DILUTING FLUID
31
how many drops should be discarded in WBC thoma
2-3 drops
32
dispensing using thoma pipette in the neubauer counting chamber.
Charging
33
locate the ruled area for WBC counting by focusing the microscope using?
LPO
34
INVERTED L-shape METHOD
WBC
35
Count the WBCs seen on the 4 secondary squares designated as W squares with an area of ?
4 mm2
36
Record the count on each of the 4 secondary squares making sure that the cell difference between the squares is ?
12 or less
37
Normal Range: 4,500 – 11,500 WBCs / mm³
WBC
38
4 𝑚𝑚² 𝑋 1/10 𝑚𝑚 𝑋 1/20
divide mo total number of cells counted WBC
39
Nature sample Operator’s error Due to problematic equipment Internal / field errors
sources of errors
40
The number of erythrocytes contained in 1 liter of blood is
erythrocyte number concentration.
41
what is the Characteristic of a good RBC diluting fluid?
ISOTONIC
42
Best RBC Diluting Fluid (cell shape is not altered) Can be stored for long periods of time
DACIE’s/ Formol citrate
43
composition of formol citrate
40% solution of formaldehyde = 10.0mL 3% W/V trisodium citrate = 990.0mL
44
Not much recommended ● No corrosive effect ● Allows growth of Yeast ● Produces clumping of cells
HAYEMS
45
Prevents Rouleaux formation Precipitates protein in hemoglobinemia and hyperglobulinemia.
GOWERS
46
Supports growth of fungi ● High specific gravity ● Can stain WBC
TOISSON’s
47
Used in emergency cases and excessive rouleaux formation and auto-agglutinated cells.
NSS
48
how many drops should be discarded in RBC Thoma?
5-6 drops
49
angle of the pipette while charging in RBC
30-35 degrees
50
RBCs that touch any of the lines on the ________ borders, even if they are outside the tertiary square, are included.
TOP AND LEFT
51
Record the count on each of the 5 tertiary squares making sure that the cell difference between two squares is
20 or less
52
All counts must be done in duplicate. TRUE OR FALSE?
TRUE
53
REFERENCE RANGE OF RBC
4.5-6.0x10¹²/L
54
Errors due to materials/equipment Operator’s technical errors Errors due to nature of sample
Sources of Error in RBC Counting
55
reference range for female for RBC
4.0-5.5 M/cumm
56
reference range for preganants
3.0-5.0 M/cumm
57
reference range for at birth
At birth = 7.0 M/cumm
58
Convenient; ○ Employs the use of pre-measured volume of diluting fluid in a plastic reservoir; ○ Specific amount of blood is collected.
unopette system