Unit 8 - Wort Boiling Flashcards

(71 cards)

1
Q

What is flavour stability in beer?

A

The beer’s ability to resist staling and maintain fresh flavour over time.

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2
Q

What causes flavour staling?

A

Lipid oxidation, heat damage, oxygen exposure, and metal ions like iron and copper.

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3
Q

How can we reduce oxidation during the boil?

A
  • Fill kettle from the bottom to limit oxygen mixing
  • Use gas sparging during fill and boil (if available)
  • LOX enzyme is inactive above 67°C, so not active during boiling
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4
Q

What’s the 5 goals for boiling wort?

A
  • Remove DMS
  • Coagulate proteins
  • Isomerise hop acids
  • Sterilise wort
  • Denature enzymes
    Too much heat → stale flavours.
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5
Q

Are reductones from extra boiling helpful?

A

Not really—any antioxidant effect is offset by creation of staling precursors.

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6
Q

How do gallotannins help flavour stability?

A

They chelate (bind) metal ions (iron, copper) that speed up oxidation. Dose: 2–6 g per hL. Also improve haze stability.

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7
Q

How does trub formation help?

A

Trub traps lipids, removing oxidation precursors. To maximise trub:
* Keep pH around 5.2
* Encourage good boil & vapour bubbles
* Avoid excessive shear forces
* Use kettle finings or gallotannins

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8
Q

How do we measure if these controls work?

A
  • EPR spectroscopy
  • GC-MS (chemical analysis)
  • Sensory testing over time
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9
Q

Enzymes are denatured below boiling temperatures. (True/False)

A

True and False. Most malt enzymes are denatured below boiling (e.g., α-amylase at ~80°C), but some commercial enzymes are heat-stable and require boiling to be fully denatured.

Enzymes play a crucial role in the mashing process and their stability can vary based on temperature.

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10
Q

Boiling destroys all microorganisms in wort. (True/False)

A

False. Boiling kills all vegetative bacteria and fungi, but spores may survive. Fortunately, spore-forming microbes are rarely beer spoilers.

Understanding the effectiveness of boiling is essential for ensuring the microbiological quality of the wort.

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11
Q

It is best to achieve the evaporation of at least 15% of the wort volume during the boil. (True/False)

A

False. 15% is excessive. A target of around 4% is typical in modern breweries. The goal isn’t evaporation itself, but achieving the necessary boiling reactions without wasting energy or harming flavour and foam stability.

Efficient boiling is key to preserving the quality of the beer while optimizing production.

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12
Q

Reactions involving amino acids and reducing sugars are the only source of flavour during the boil. (True/False)

A

False. Hop compounds (bitterness and aroma), adjunct sugars (like candi sugar), and mild caramelisation also contribute to boil flavour.

The complexity of flavour development during boiling is vital for creating a well-rounded beer.

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13
Q

DMS can form after wort boiling is complete. (True/False)

A

True. SMM can break down into DMS during wort clarification, and yeast can reduce DMSO to DMS during fermentation.

Monitoring DMS levels is important for the final flavor profile of the beer.

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14
Q

What is the optimum pH at the end of wort boiling?

A

Optimum pH = 5.0–5.3

Correct pH helps improve protein coagulation, diacetyl reduction during fermentation, yeast growth, faster fermentation, inhibit contaminants, reduce colour formation, and achieve correct beer pH.

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15
Q

Why is ensuring that the pH is correct during the boil important?

A

Correct pH helps:
* Improve protein coagulation
* Improve diacetyl reduction during fermentation
* Encourage yeast growth
* Enable faster fermentation
* Inhibit contaminants
* Reduce colour formation
* Achieve correct beer pH

Note: hop utilisation is not optimal in this pH range.

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16
Q

What is the formula to calculate percentage evaporation if the start of boil gravity is 9.7°P and end of boil gravity is 10.6°P?

A

Evaporation = ((10.6 – 9.7) / 10.6) × 100 = 8.5%

This is acceptable (4–10% range), but there’s room for optimisation to save energy, reduce cost and improve flavour stability.

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17
Q

When do kettle finings need to be optimised?

A

Kettle finings need to be optimised regularly and whenever process/raw materials change or wort clarity drops

Balance: wort clarity and compactness of sediment in the Imhoff cone.

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18
Q

What are the two parameters that need to be balanced when optimising kettle finings?

A

Balance:
* Wort clarity
* Compactness of sediment in the Imhoff cone

Aim = clear wort + compact sediment.

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19
Q

How can you improve the utilisation of T90 hops during wort boiling?

A

To improve utilisation:
* Increase wort pH
* Reduce trub volume
* Increase boil time or temperature
* Lower wort gravity
* Use higher α-acid hops
* Reduce foaming (fobbing)
* Increase boil vigour

Some options may compromise other quality goals.

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20
Q

What is the DMS level at the end of wort boiling and its potential effect on the beer?

A

220 μg/L is well above DMS flavour threshold (30–50 μg/L)

Beer will likely have unwanted DMS aroma.

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21
Q

What short-term action can you take if the wort DMS is high?

A

Blend with a lower-DMS brew

This is a short-term solution to mitigate the DMS aroma.

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22
Q

What are the long-term actions to take for future brews to manage DMS levels?

A

Long-term actions:
* Use malt with low SMM
* Ensure enough heat to convert SMM to DMS
* Use an efficient wort boiling system
* Minimise hot residence time after boil
* Cool wort quickly
* Consider wort stripping after clarification

These actions help reduce DMS in future brews.

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23
Q

What are common raw materials and process aids added during wort boiling?

A

Adjuncts, hops/hop products, kettle finings, gallotannins, silica sol, PVPP, antifoams, yeast food, calcium or magnesium salts, sour wort, and acids.

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24
Q

What role do adjuncts play when added during the wort boil?

A

Increase wort gravity, influence flavour, colour, and flavour stability; can dilute malt flavour, allow high-gravity brewing, and expand brew size for same malt input.

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25
What role do hops and hop products play during the boil?
Provide bitterness and aroma; aid trub formation via polyphenols and vegetable matter; help reduce fouling on heating surfaces.
26
What is the purpose of kettle finings in wort boiling?
Improve trub formation and cold break; enhance protein removal, improving beer filterability and colloidal stability.
27
What do gallotannins do during wort boiling?
Help remove haze proteins and prooxidant metal ions (copper and iron).
28
What does silica sol do when added to boiling wort?
Adsorbs haze proteins and polypeptides, aiding in their removal via trub.
29
Why is PVPP added during the wort boil?
Removes polyphenols that can cause haze formation.
30
What is the risk of overdosing clarification agents during wort boiling?
Can lead to excessive cold break → harms yeast health and increases beer losses.
31
What is the function of calcium salts during wort boiling?
React with phosphate to lower pH and remove oxalic acid (preventing calcium oxalate scale and gushing).
32
Why is magnesium less effective than calcium for pH reduction?
Magnesium causes similar reactions, but the pH drop is less significant than with calcium.
33
What does yeast food provide during wort boiling?
Supplies nutrients (especially zinc) for healthy fermentation—critical in sugar-adjunct worts; added at boil end for sterility.
34
Why are acids or sour wort added during the boil?
Adjust wort pH. Phosphoric acid addresses phosphate deficiency; lactic acid or sour wort offers smoother flavour.
35
What is the purpose of antifoam during wort boiling?
Reduces foam formation in the kettle, preventing bitterness loss and potentially preserving foam stability.
36
What aspect of the wort boil does reducing evaporation from 8% to 4% most likely affect?
The amount of thermal energy applied to the wort, which can impact multiple aspects of wort and beer quality.
37
Why is it important to evaluate wort quality after changing the boil evaporation rate?
Because boil outcomes depend on thermal energy input, and reducing evaporation may affect enzyme inactivation, sterilisation, flavour, colour, protein precipitation, and volatile removal.
38
How can you confirm that malt enzymes have been inactivated in the 4% evaporation trial?
HPLC sugar analysis or wort attenuation limit test—but usually unnecessary, as 4% evaporation still applies sufficient heat.
39
How can you confirm wort sterilisation after the boil?
Wort forcing test (27°C for 7 days) or membrane filtration followed by culturing on nutrient agar. Often unnecessary for 4% evaporation.
40
How do you measure whether evaporation and concentration of the wort occurred as expected?
Use a densitometer or hydrometer to measure gravity before and after the boil, or track steam/energy use, or wort level change.
41
How is wort colour development measured?
Spectrophotometer at 430 nm or visual comparator.
42
How can wort flavour development be analysed after changing boil conditions?
GCMS comparison of wort volatiles and sensory analysis using a trained tasting panel.
43
How do you measure isomerisation and extraction of hop compounds?
Measure bitterness with 2,2,4-trimethylpentane extraction and spectrophotometry, or HPLC. Hop aroma via GCMS.
44
How do you evaluate the formation and removal of protein-polyphenol complexes during boiling?
Imhoff cone sediment test, Kjeldahl method to measure coagulable nitrogen before and after boil.
45
What impact does reduced evaporation have on beer haze and fermentation, and how is it tested?
Less protein removal may lead to haze or yeast coating. Test with haze forcing, saturated ammonium sulphate test, fermentation rate, and yeast viability checks.
46
What is the key unwanted volatile that must be removed during boiling, and how is it tested?
Dimethyl sulphide (DMS); measured using GCMS.
47
What aldehydes should be tested to assess boil effectiveness in volatile removal?
* Isobutyraldehyde * 3-methylbutanal * 2-methylbutanal * 3-methylthiopropionaldehyde
48
How can the condensate be used to assess removal of unwanted volatiles?
Analyse condensate via GCMS for aldehydes, ketones, pyrazines, sulphides, or do odour tests—early condensate should be worty; late condensate odourless.
49
How do you measure wort acidification after boiling?
Measure pH using a pH meter.
50
How is activation of foam-stabilising proteins assessed?
NIBEM test for foam stability over time; advanced labs can measure Protein Z and LTP1 levels.
51
What is the formula to calculate energy required to heat a substance?
q = M × Cp × (T2 - T1) ## Footnote Where q is the energy, M is the mass, Cp is the specific heat capacity, and (T2 - T1) is the temperature change.
52
In the equation q = M × Cp × (T2 - T1), what does 'M' represent?
Mass of the substance in kg
53
What does Cp stand for and what are its units?
Specific heat capacity (kJ/kg°C), energy needed to heat 1 kg by 1°C
54
What does (T2 - T1) represent in the energy equation?
Temperature rise (°C or K)
55
What is the specific heat capacity of wort used in brewing calculations?
4.0 kJ/kg°C
56
How do you calculate the mass of 1000 hL of wort with density 1.032 kg/L?
1000 hL = 100,000 L → 100,000 × 1.032 = 103,200 kg
57
How much energy is needed to heat 103,200 kg of wort from 75°C to 100°C?
q = 103,200 × 4.0 × 25 = 10,320,000 kJ = 10,320 MJ
58
If it takes 25 min to heat the wort, what is the heat rate in MJ/min?
Q = 10,320 MJ ÷ 25 = 412.8 MJ/min
59
How do you convert MJ/min to kW?
Divide by 60 (sec) and multiply by 1000 → (MJ/min ÷ 60) × 1000
60
What is the kW value of 412.8 MJ/min?
6880 kW
61
What is the specific heat of vaporisation of water?
2257 kJ/kg
62
How much water is boiled off during 5% evaporation of 100,000 L?
5% of 100,000 = 5000 L = 5000 kg
63
How much energy is needed to boil off 5000 kg of water?
5000 × 2257 = 11,285,000 kJ = 11,285 MJ
64
What is the energy rate (Q) for this boil over 60 minutes in MJ/min?
11,285 MJ ÷ 60 = 188.08 MJ/min
65
What is the kW equivalent of 188.08 MJ/min?
(188.08 ÷ 60) × 1000 = 3134.67 kW
66
Why does boiling require more energy but less kW than heating?
Boiling happens over a longer time, spreading the energy out
67
What is the formula for heat transfer during boiling?
Q = U × A × ΔT
68
In the heat transfer equation, what does 'U' represent?
Overall heat transfer coefficient (kW/m²K)
69
What does 'A' represent in Q = UAΔT?
Heat transfer surface area in m²
70
What does ΔT represent in Q = UAΔT?
Temperature difference between heating surface and wort (K)
71
What 3 things increase heat transfer rate (Q) during boiling?
* High U (efficiency) * Large A (surface) * Large ΔT (temp difference)