Unit Fi Flashcards
(68 cards)
What are the stages of photosynthesis?
- Light dependent reaction
- Light independent reaction
Where does the light-dependent reaction occur?
Thylakoid membrane of chloroplast
What happens during photoionisation in the light-dependent reaction?
Chlorophyll absorbs light energy, exciting electrons and releasing them.
What is produced from the energy released during photoionisation?
ATP and reduced NADP
What is photolysis of water?
Water splits to produce protons, electrons, and oxygen (H2O → ½ O2 + 2e- + 2H+)
What is the role of rubisco in the Calvin cycle?
Catalyses the reaction of CO2 with ribulose bisphosphate (RuBP)
What happens to glycerate 3-phosphate (GP) in the Calvin cycle?
GP is reduced to triose phosphate (TP) using reduced NADP and ATP.
How does temperature affect the rate of photosynthesis?
Rate increases with temperature until an optimum, then decreases due to enzyme denaturation.
What is the effect of light intensity on photosynthesis?
Rate increases with light intensity until another factor becomes limiting.
How does CO2 concentration affect the rate of photosynthesis?
Rate increases with CO2 concentration until another factor becomes limiting.
What is a key consideration when evaluating agricultural practices related to photosynthesis?
Profit from increased yield should exceed costs.
What is the purpose of paper chromatography in isolating pigments?
To separate and identify pigments from leaves.
Describe the process of glycolysis.
- Glucose phosphorylated to glucose phosphate
- Hydrolysed to 2 x triose phosphate
- Oxidised to 2 pyruvate
What occurs during anaerobic respiration after glycolysis?
Pyruvate is converted to lactate or ethanol, regenerating NAD.
Why does anaerobic respiration produce less ATP than aerobic respiration?
Only glycolysis occurs, producing limited ATP.
What happens to pyruvate in aerobic respiration?
It is actively transported into the mitochondrial matrix.
What are the products of the link reaction per glucose molecule?
2 x Acetyl Coenzyme A, 2 x CO2, 2 x reduced NAD
What are the main products of the Krebs cycle per glucose molecule?
6 x reduced NAD, 2 x reduced FAD, 2 x ATP, 4 x CO2
Describe the oxidative phosphorylation process.
- Reduced NAD/FAD oxidised
- Electrons transferred down electron transfer chain
- ATP produced via chemiosmosis
- Oxygen is the final electron acceptor
Give examples of other respiratory substrates.
Breakdown products of lipids and amino acids.
What is the role of dehydrogenase in photosynthesis?
Catalyses the reduction of NADP in the light-dependent reaction.
How can the rate of dehydrogenase activity be measured?
By timing the decolourisation of DCPIP in the presence of chloroplasts.
What is the purpose of control in experimentation?
To isolate the effect of the variable being tested.
What is the significance of using Rf values in chromatography?
Rf values allow for comparison of pigments under consistent conditions.