Untitled Deck Flashcards
(15 cards)
What are the two main types of ingredients in tissue culture media?
Basal and essential ingredients.
What are some common basal media used in tissue culture?
Eagle minimal essential medium (MEM), Dulbecco’s modified Eagle medium (DMEM), Roswell Park Memorial Institute (RPMI 1640), and others.
What nutrients are typically found in basal media?
Amino acids, glucose, salts, vitamins, and other nutrients.
What are the energy sources essential for cell growth?
Glucose and galactose.
Why are vitamins important in tissue culture media?
They are precursors for numerous co-factors and necessary for cell growth and proliferation.
Which common vitamins are found in basal media?
Riboflavin, thiamine, and biotin.
What trace elements are essential for cell viability?
Zinc, copper, and selenium.
What is the most important ingredient in tissue culture media?
Fetal calf serum.
How is cell viability defined?
The level of health of cells indicating capability of division.
How is cell viability determined?
By staining the cells with trypan blue dye.
What does trypan blue dye indicate?
It stains only non-viable cells.
How can cell viability be calculated?
As a percentage of total cell count.
What equipment can be used for automated cell counts?
Cellometer.
What are the conditions provided by a carbon dioxide (CO2) incubator for mammalian cells?
Temperature of 37°C, 2-5% CO2, and 95% air.
What is the purpose of double jacket walls in CO2 incubators?
To maintain 99% relative humidity and avoid dryness of cultures.
