Urinary Tract and Mouth

Question 1

Quantitative analysis

Answer 1

determine the number of bacteria per

mL of urine

Question 2

Qualitative analysis

Answer 2

examine the features of bacteria that

grow in urine

Question 3

What is a Calibrated Plate?

Answer 3

With Calibrated loop one streak down the middle and then a streak across the entire plate.

Question 4

Cariogenic bacteria can cause…

Answer 4

bacteria that can lead to tooth decay
and dental carries (cavities)
- Produce a capsule a stick to teeth
- Ferment sugars to produce acids a dissolve tooth

Question 5

Species Streptococcus salivarius Sucrose Components?

Answer 5

Glucose= ACID
Fructose= CAPSULE

Question 6

Species Streptococcus mutans Sucrose Components?

Answer 6

Glucose=Capsule

Fructose=ACID

Question 7

Sucrose plate is…

urinary

Answer 7

differential for capsule
production
colonies appear gooey/
slimy, moist, glistening, dome shaped

Question 8

Snyder agar is…

urinary

Answer 8

differential for fermentation
Green = (-)
Yellow = (+)

Question 9

Why are women more likely to get a UTI than men?

Answer 9

1. Urethra is shorter in women

2. Anal opening is closer to the urethra in women

Question 10

10,000-100,000 bac/mL

is considered?

Answer 10

false positive and the patient is

negative for a UTI

Question 11

What could lead to a false positive?

Answer 11

• Patient didn’t wipe properly before giving sample
• Patient didn’t get a mid-stream catch
• Sample was not tested in a timely manner by the lab

Question 12

Calibrated loop holds how much fluid?

Answer 12

1uL

Question 13

What is 1uL in mL

Answer 13

0.001mL

Question 14

What type of dilution can be made using a calibrated loop?

Answer 14

1x10^3 dilution

Question 15

How many colonies are required on a calibrated loop to

represent 100,000 bac/mL in the original sample?

Answer 15

100 colonies = UTI

Question 16

EMB plate is…

Answer 16

• Selective: Gram negative

- Differential: Lactose fermentation

Question 17

Blood agar plate plate is…

Answer 17

• Selective: nothing

- Differential: hemolysis

Question 18

Gamma =

Answer 18

no hemolysis

Question 19

Alpha =

Answer 19

partial hemolysis

Question 20

Beta =

Answer 20

complete hemolysis

Question 21

Reducing media…

Answer 21

• Contains thioglycollate which removes oxygen

- E. coli = facultative anaerobe (grows throughout)

Question 22

Sucrose Plate is…

mouth

Answer 22

• Differential for capsule production

- Look for gooey or sticky looking colonies

Question 23

Snyder Agar is…

mouth

Answer 23

• Differential for fermentation
• Look for yellow color change à timing of color change
  matters!
• Determine your risk for dental carries

Question 24

Risk of dental caries
Timing of Yellow appearance in Snyder Agar

Within 24 hours?

Answer 24

High risk

Question 25

Risk of dental caries Timing of Yellow appearance in Snyder Agar 24-48 hours

Answer 25

Moderate risk

Question 26

Risk of dental caries Timing of Yellow appearance in Snyder Agar 48-72 hours

Answer 26

Low risk

Question 27

Risk of dental caries Timing of Yellow appearance in Snyder Agar After 72 hours

Answer 27

Negative result