virus cultivation Flashcards
stock material for virus cultivation
CO2 gassed incubator laminar flow cabinet inverted microscope refrigerator and freezer centrifuge thermostatic bath
why do we need co2 gassed incubator for virus cultivation?
viruses need to be cultivated in living cells in 37 C with 3-10% CO2. this can be acheived with co2 gassed incubator
why do we need laminar flow cabinet for virus cultivation?
it filters the air so we can avoid any contamination
inverted microscope
useful for observing cell cultures at the bottom of a large container
thermostatic bath
maintains the 37 C uniformely
consumable material for virus cultivation
culture materials
pipettes
centrifuge tubes
culture media
culture material
sterile and disposable
made of plastic
cell culture flasks
microscope slides with reaction wells
well dishes
culture plates
pipettes
sterile pipettes with cotton at the top
automatic pipettes
micropipettes
sterile tips
culture media for virus cultivation
RPMI-1640
DMEM
containing FetalBovineSerum
l-glutamate
antibiotics
antifungal agents
types of cell lines
adherent and suspension cells
adherent cells
in vitro grow adherent to the plastic until they have covered the area or used all the nutrients from the medium
suspension cells
blood-derived cells growing in suspension in vitro
subculturing a cell line is done
always inside the laminar flow cabinet
cytopathic effects of viruses in cultures
cell lysis syncytium formation round cells as bunches of grapes vacuoles inside the cytoplasm detachment of the cell monolayer inclusion bodies
virus presence tests
hemadsorption immunofluorescence elisa western blot hai pcr
