Viruses + virology Flashcards
(23 cards)
Contagium vivum fluidium
Soluble living germ - virus
Obligate intracellular parasites
Hijack host
Require host to reproduce + metabolise
Structural genes
Encode proteins that make viral structures
Non-structural genes
Encode proteins for virus function
Viriods
No protein component
Smallest self replicating pathogen
Rod/dumbell shape
In vivo
In organism
In vitro
In cells
CPEs
Changes in morphology of infected cell due to virus growth and replication
HA assay
Cross linking of viruses + sialic acid in RBCs
Agglutination
Plaque assay
Dye added
Clear areas = plaques
1 plaque =
1 PFU
Vaccine types - live attenuated
Weakened whole virus
Can replicate
Vaccine types - killed
Suitable for immunocompromised
Vaccine types - subunit
Specific components
VAP/capsid/antigen expressed in bacteria/yeast
Antiviral types - binding inhibitors
Block virus-host receptor binding
Antiviral types - entry inhibitors
Block fusion/entry
Antiviral types - polymerase inhibitors
Terminate chain synthesis
Terminate replication
Phosphorylation by viral kinase -> activation of antiviral to only work in infected cells
Antiviral types - non-polymerase inhibitors
Block viral protease activity
Antiviral types - neuraminidase inhibitors
Prevent virion release
Icosahedral capsid structure
20-sided symmetrical capsid
Helical capsid structure
Rod-shaped
Made of NA
Complex capsid structure
Icosahedral head
Helical tail
Disease classification groups
Hepatitis viruses (liver)
Common cold viruses (upper tract)
Respiratory viruses (lower tract)
Enteroviruses (GI)
STV
