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Flashcards in Vwf Deck (10):
1

What does vwf:cba detect?

ability of vwf to bind to collagen
measures preferentially the High Molecular Weight [HMW] multimers of VWF

2

Method CBA

Elisa- wells coated w Human collagen type III
Plasma added
Washed
HRP added to deft ab present by binding causing colour change
Amy colour proportional,to amt vwf bound to collagen

3

Dx of type 2n requires?

Pedigree analysis
Genetics
FVIII binding assa
Measure both fviii and vwf activity

4

How do,you ensure vwf assays remain précis

Use of controls w known vwf concentration to gen curves

5

Other DDx for mucosal bleeding

Acquired VWD,
platelet-type VWD,
platelet function defects,
vascular malformations such as hereditary hemorrhagic telangiectasias
connective tissue disorders

6

Which r on plts does vwf bind to

to platelet glycoprotein Iba

7

Pt related rf for inhibitor development

Race
Fhx
FVIII mutation
MHC class
Polymorphism of immune response genes- il 10, TNF, CTLA4

8

How does fviii work

Travels bound to Vwf and when act by fvii will activate fix

9

How is FVIII cleared

By fv and fix
Short half life

10

Riusk factor for F5 inhibitors

by exposure to bovine protein (i.e. bovine thrombin preparations)
Antibodies are generated as an immune response to bovine factor V which then cross-reacts with
recent surgery,
drug exposure (e.g. aminoglycosides, B-lactam antibiotics),
infections,
blood transfusions,
autoimmune disorders,
malignancy
pregnancy