WBC

Question 1

eosinophils

Answer 1

allergic response/some phagocytosis
12-17 u in diameter
cyto filled w/ red-orange granules (much larger than neut.)
dense nuclear chromatin
segmented nucleus

Question 2

neutrophils

Answer 2

eliminate bacteria by phagocytosis/ inflammatory rxn
10-14 u
clear cyto with pink to lavendar granules
dense nulcear chromatin
segmented nucleus

Question 3

basophils

Answer 3

allergic rxn/ hypersensitivity rxns
10-14 u
cytoplasm filled w/ large blue-black granules
dense nuclear chromatin
segmented nucleus

Question 4

lymphocytes

Answer 4

Tcell regulate AB syn., immune response
Bcell synthesize AB, immune response
dense nuclear chromatin
round-oval nucleus
blue cyto. 
\+/- azurophilc granules

Question 5

reactive lymphocytes

Answer 5

blue around edges
indented by RBCs
not reported if less than 10% of cells

Question 6

monocytes

Answer 6

phagocytosis, immune response, ingest & kill microorganisms, clear aged/damaged cells
15-22 u
blue-grey cytoplasm- ground glass
irregular nuclear shape (horseshoe, bent)
less dense nuclear chromatin
most likely to be vacuolated

Question 7

complete differential includes:

Answer 7

wbc estimate
wbc differential
rbc morphology
plt estimate
calculation of wbc absolute values

Question 8

normal values segmented neutrophils

Answer 8

40-80%

Question 9

normal values band neutrophil

Answer 9

0-5%

Question 10

normal lymphocyte value

Answer 10

25-35%

Question 11

normal monocyte value

Answer 11

2-10%

Question 12

normal eosinophil value

Answer 12

0-5%

Question 13

normal basophil value

Answer 13

0-1%

Question 14

normal absolute value segmented neutrophil

Answer 14

1.8- 7 x 103/cumm (or 109/L)

Question 15

normal absolute value band neutrophils

Answer 15

0-0.7 x 103/cumm (or 109/L

Question 16

normal absolute value lymphocytes

Answer 16

1-4.8 x 103/cumm (or 109/L)

Question 17

normal absolute value monocytes

Answer 17

0.2- 0.8 x 103/cumm (or 109/L)

Question 18

normal absolute value eosinophil

Answer 18

0- 0.4 x 103/cumm (or 109/L)

Question 19

normal absolute value basophils

Answer 19

0- 0.2 x 103/cumm (or 109/L)

Question 20

wbc differential sources of error

Answer 20

non random cell distribution
individual variation in cell id
variation in method
inherent error in evaluating 100 cells
Rumke's table (statistical test takes error into account)

Question 21

clinical significance of neutrophilia

Answer 21

younger forms seen as severity increase
bacterial infection
inflamm

Question 22

clinical significance of neutropenia

Answer 22

drug induced (absolute)
viral infection (relative)
overwhelming infection

Question 23

clinical significance of lymphocytosis

Answer 23

viral infec
leukemia
childhood (adult levels @ puberty)

Question 24

clinical significance of lymphocytopenia

Answer 24

drug induced (absolute)
bacterial infec (relative)

Question 25

clinical significance of monocytosis

Answer 25

fungal infec (most often) leukemia TB (most often) post chemo

Question 26

clincial significance of eosinophila

Answer 26

allergy asthma parasitic infec leukemia (rare)

Question 27

clinical significance of basophilia

Answer 27

allergy hypersensitivity rxn leukemia (extremely rare)