Week 11

Question 1

Fab domain of antibody

Answer 1

Binds to epitope

Question 2

Fc domain of antibody

Answer 2

Biological effector

Modulate protein function

Question 3

How are monoclonal antibodies made using mouse?

Answer 3

Antigen injected into mouse —> immine response harvested —> mix with immortalised myeloma cells —> fused into hybridoma cells (via salvage pathway) —> HAT medium selection

Question 4

Monoclonal antibody production (no mous)?

Answer 4

Cell in well with growth factor —> antibodies secreted in medium —> test reaction w antigen —> propagate positive —> isolate and purify

Question 5

Humanising process (3)

Answer 5

1 100% mouse antibody

2 chimera. Human comstant regions, mouse variable regions.HAMA response reduced

3 mostly human, HAHA response. CDRs only from mouse.

Question 6

Phage display

Answer 6

Isolate human b cells and transfect into animals

Question 7

Human system for antibodies

Answer 7

Find survivor of disease and try to immortalise b cell to find antibody