Week 2: Bacterial Metabolism/Genetics Flashcards
(28 cards)
how many ATP molecules are generated from glycolysis of glucose?
2 ATP
terminal electron receptor can be…
oxygen
what molecule makes oxygen free radical (O2-) to peroxide?
superoxide dismutase
what decomposes peroxide to water?
catalase and peroxidases
do aerobes need O2 for terminal electron receptor?
yes
_____ prefer to use ____ if available, but can use fermentation
facultatives, oxygen
microbe that doesn’t use O2, but are OK if it’s around
aerotolerant
microbe that…
- lack superoxide dismutase and catalase
- ferment or use nitrate, sulphate, etc. for e-
strict anaerobes (obligate)
microbe that likes a little O2 (5%)
microaerophiles
optimal growth temp of mesophiles
25-40 degrees C
scale of temperature/bacteria
psychrophile psychrotroph mesophile thermophile hyperthermophile
neutrophiles like a…
neutral pH
microbes that like acidic pH
acidophiles
microbes that like basic pH
alkalophiles
types of sampling
- aspirated pus/swab/paper point
- clean sample
- smear
- culture
clean sample keys
- target site infection
- reduce level of contamination
what can be defined as the persistence of genetic information?
life
central dogma of molecular biology/genetics
DNA –> RNA –> Protein
description of bacterial cells
- single
- circular
- double-stranded DNA
are bacteria haploid or diploid?
haploid
which cell is larger? human or bacterial cell?
human cell, in many apsects
lac protein that transports lactose into cells
lacY
lac enzyme that breaks down lactose into monomers that can be used in glycolysis
lacZ
DNA sequence that RNA polymerase binds to
promoter
