Week 3

Question 1

Effect of PRR signaling

Answer 1

Cytokine and chemokine production

Increase exp of costimulatory mol: B7.1(CD80), B7.2 (CD86)

Enhanved mgration to regional secondary lymphoid organ

Question 2

Cytokine produyction (effect of PRR signaling)

Answer 2

IL-1, IL-6, IL-18alpha, IL-12: Inflammation

TYPE 1 IFN(IFN-alpha, IFN-beta): potent antiviral effect:

Type 1 interferon (IFN)=antiviral effect and is secreted upon PRR signaling

Binds to receptor on surroundinf ¢ to trigger intra¢re signaling so gene can be expressed(transc factor or
gene that inhibit viral replication so can halt/slow viral infection)

Question 3

Chemokines

Answer 3

CC chemokine (CCL2) and CXC chem (ex. CXCL8 or IL-8): differnce in theri location of two cystein residue

Chemokine receptor=G-Protein coupled receptor: transduce signal via interaction w/ GDP/GTP binding G P
Many receptor can bind more than one chemokine and many chemokine can bind to more than one receptor

Chemokines direct leukocyte migration (chemotaxis): signaling thru chemokine receptors helps cells move to a different areas: change in cell adhesiveness, change in £ cytoskeleton, induce mvt of leukocyte up a chemokine concentration gradient

Question 4

Migration and costimulation

Answer 4

PRR singaling triggers migration and targetin gof DC to lymphoid tissue

Increased expression of costimulatory molecule (CD80, CD86 aka B.1/B7.2)

Question 5

Study of erreots of TLR-3 AND IRF7 dependent type I-IFN production and amplifiaction

Answer 5

2groups: asymptomatic and life-threatening COVID, and the last couldnt make type 1 interferon vs asymptmatic that had them

Question 6

Migration of innate to site of infection

Answer 6

Check pic 1 fofr general summary

Question 7

Monocyte recruited to site of infection aadhesin molecule

Answer 7

check pic 2

Question 8

Adhesion molecule

Answer 8

Diff families (selectins, integrins, immunoglobulin superfamily)

Diff tissue distribution of these adhesiomn mol

Some adhesion mol ar expressed at baseline (always) some other are expressed a lot or only upregulated when there’s infection

Question 9

Adhesion mol: leukocytes roll along vascular endothelium

Answer 9

rolling due weak interactions alon endothelial

Selectin-mediated adhesion
PIC3

Question 10

REcruitement of leukocyte

Answer 10

Monocyte(precurosor of macrophage), neutrophils and other leukocyte recruited to site of infection

HEat, redness, swelling (edema), pain, vasodilation+inflammation SHOULD ALWAYS BE ACUTE TO CLEAR IT…Sever or chornic inflmmation: arthritis or loss of fct

PIC 4 very impo

Question 11

mIGRATION STEPS

Answer 11

Check pic 5

Rolling adhesion: rolling due to adhesion

Tight binding: anchoring takes place

Diapedesis: process ¢ crosses from lumen of a vessel between endothelial ¢ into surrounding tissue

Migration in the directino of higher chemokine[]