Week 5 Flashcards
1
Q
When extracting DNA from cells, why is SDS in the lysis solution
A
It allows the lipids to mix with the water
2
Q
After exposure to the electric current, the gel is immersed in a warm stain solution. Why do we keep this solution warm?
A
When the gel gets warmer, the faster it’ll diffuse the stain into the gel
3
Q
Explain distancing when measuring band fragments
A
The smaller the fragment the further the distance
4
Q
What are the four bases that are found in DNA
A
Adenine, thymine, guanine, cytosine
5
Q
A sample of DNA is 100,000 bases long. It is cut between bases 25,000 and 25,001, and between bases 50,000 and 50,001. This DNA is then loaded into a gel and exposed to an electric current (electrophoresis). How many bands of DNA would result?
A
2 bands
