White Blood Cell Microscopic Identification

Question 1

• A heterogenous group of nucleated cells found in the blood circulation

Answer 1

White Blood Cells (leukocytes)

Question 2

WBCs
• Play an important role in_______ of the body

Answer 2

immunity

Question 3

• Classifications

Answer 3

Granulocyte
Agranulocyte

Question 4

Granulocyte

Answer 4

Neutrophil
Eosinophil
Basophil

Question 5

Agranulocytes

Answer 5

Lymphocyte
Monocyte

Question 6

NEUTROPHIL
• In the circulation:
• Size:

Answer 6

Mainly mature segmented neutrophils

10-14 um

Question 7

NEUTROPHIL

• Nucleus:
• Cytoplasm:

Answer 7

Central or eccentric blue violet 3-4 lobes, connected by chromatin thread

Pale blue to pink

Question 8

NEUTROPHIL

• Nucleus: Central or eccentric (color and number of lobes), connected by_____

• Cytoplasm: Pale blue to pink

Answer 8

blue violet 3-4 lobes

chromatin thread

Question 9

NEUTROPHIL

Cytoplasmic granules

Answer 9

Azurophilic, fine, closely packed, violet-pink/ fine lilac to pink, not seen separately, and do not cover the nucleus

Question 10

Azurophilic, fine, closely packed, violet-pink/ fine lilac to pink, not seen separately, and do not cover the nucleus

Answer 10

Neutrophil

Question 11

NEUTROPHIL

• Function: Defense against ______
• Life span: ______ (circulation); _______(tissues)

Answer 11

Defense against bacterial
infection (phagocytosis)

6-10 hrs (circulation);
5 days (tissues)

Question 12

• Function: Defense against bacterial
infection (phagocytosis)
• Life span: 6-10 hrs (circulation); 5 days (tissues)

Answer 12

Neutrophil

Question 13

immature neutrophil

Answer 13

Band cell

Question 14

BAND CELLS

• In the circulation:
• Size:

Answer 14

small in number

10-14 um

Question 15

BAND CELLS

• Nucleus:
• Normally account for approx.____ of peripheral blood leukocytes

Answer 15

non-segmented, C or S shaped nuclei

5-10%

Question 16

• Nucleus; non-segmented, C or S shaped nuclei
• Normally account for approx. 5-10% of peripheral blood leukocytes

Answer 16

Band cells

Question 17

NEUTROPHIL GRANULES

Answer 17

• Proteases
• Oxidizing agents
• NADPH oxidase
• Defensins
• Lysozymes
• Myeloperoxidases

Question 18

• BI-LOBED

Answer 18

EOSINOPHIL

Question 19

EOSINOPHIL

• Size:
• Nucleus:
• Cytoplasm:

Answer 19

10-14 um

Central or eccentric purplish blue 2 lobes, connected by a thick chromatin thread, “spectacle shaped”

acidophilic, light pink to red

Question 20

• Size: 10-14 um

• Nucleus: Central or eccentric purplish blue 2 lobes, connected by a thick chromatin thread,
“spectacle shaped”

• Cytoplasm: acidophilic, light pink to red

Answer 20

Eosinophil

Question 21

• Cytoplasmic Granules: Eosinophilic, large, coarse, uniform sized, brick red to orange, seen separately, does not cover nucleus

Answer 21

Eosinophil

Question 22

• Function: involved in allergy (neutralize basophil and mast cell products) and parasitic infections

• Life span: 6-10 hrs (circulation); 5 days (tissues)

Answer 22

Eosinophil

Question 23

Eosinophil

• Function: involved in ______ (neutralize basophil and mast cell products) and______

• Life span:
_______ (circulation);______(tissues)

Answer 23

allergy; parasitic infections

6-10 hrs

5 days

Question 24

EOSINOPHIL GRANULES

Answer 24

• Major basic protein
• Acid phosphatase
• Arylsulfatase
• Eosinophilic cationic protein
• Eosinophil derived neurotoxin
• Eosinophil peroxidase

Question 25

BASOPHIL GRANULES

Answer 25

• Bradykinin • Eosinophil chemotactic factor • Serotonin • Heparin • Histamine

Question 26

BASOPHIL • Size: • Nucleus: • Cytoplasm:

Answer 26

10-15 um Central, 2-3 lobes, blue-violet S-shaped, not clearly seen due to overlaying of granules Bluish, basophilic, full of granules

Question 27

• Size: 10-15 um • Nucleus: Central, 2-3 lobes, blue-violet S- shaped, not clearly seen due to overlaying of granules • Cytoplasm: Bluish, basophilic, full of granules

Answer 27

BASOPHIL

Question 28

• Cytoplasmic Granules: Large, very coarse, variable in size, deep bluish purple, observed separately, completely fill the center and obscure the nucleus

Answer 28

Basophil

Question 29

Basophil • Function: • Life span:

Answer 29

Allergic inflammation progression and maintenance Exist for only a few hours in the bloodstream

Question 30

• Function: Allergic inflammation progression and maintenance • Life span: Exist for only a few hours in the bloodstream

Answer 30

Basophil

Question 31

• The smallest WBC • Same size as the RBCs

Answer 31

LYMPHOCYTE

Question 32

Lymphocytes • Size:_____ (Small lymphocyte);____(Large lymphocyte) • Nucleus: • Cytoplasm:

Answer 32

7-9 um; 10-15 um Eccentric, indented, large round nucleus, deep purplish blue Scanty, sparse, light blue color

Question 33

• Size: 7-9 um (Small); 10-15 um (Large) • Nucleus: Eccentric, indented, large round nucleus, deep purplish blue • Cytoplasm: Scanty, sparse, light blue color

Answer 33

Lymphocytes

Question 34

• Cytoplasmic Granules: None • Function: Defense against viral infections, specific acquired immunity, participates in cell-mediated and humoral immunity

Answer 34

Lymphocytes

Question 35

3 types of lymphocytes

Answer 35

T-cell, B-cell, Natural Killer (NK) cell

Question 36

MONOCYTE • Circulation:_____ • Tissues:_____

Answer 36

Monocyte Macrophages

Question 37

Monocytes • Size: Nucleus: • Cytoplasm:

Answer 37

12-20 um pale blue violet, large, single, indented or horse shoe or kidney shaped, occupies almost half of the cytoplasm Dull grayish blue/pale blue; "ground-glass appearance"; clear, may contain digestive vacuole

Question 38

12-20 um pale blue violet, large, single, indented or horse shoe or kidney shaped, occupies almost half of the cytoplasm Dull grayish blue/pale blue; "ground-glass appearance"; clear, may contain digestive vacuole

Answer 38

Monocytes

Question 39

• Cytoplasmic Granules: Azurophilic, fine lilac granules

Answer 39

Monocytes

Question 40

Monocytes • Function: • Life span:

Answer 40

Phagocytosis, bacterial killing, antigen presentation 70 hours (peripheral blood)

Question 41

• Function: Phagocytosis, bacterial killing, antigen presentation • Life span: 70 hours (peripheral blood)

Answer 41

Monocytes