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BACTE LAB > WK 1 LAB > Flashcards

WK 1 LAB Flashcards

(59 cards)

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1
Q

AUTOCLAVE USE

A

STERILIZATION

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2
Q

AUTOCLAVE USES IT FOR SEALING AND CREATING PRESSURE

A

RuBBER GASKETS

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3
Q

what water is used in autoclave

A

distilled or deionized water

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4
Q

to achieve complete sterilization in an autoclave what sjhould be the stats

A

121C, 15psi for 15 mins

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5
Q

used to create the mold
used in this machine.

A

petri dish or tubes

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6
Q

most commonly used biosafety cabinet

A

class 2

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7
Q

bsc for very fatal organism

A

class 3

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8
Q

USED TO CONFINE DROPLETS OR BACTERIA

A

BSC

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9
Q

FILTER OF BSC

A

HEPA (high-efficiency particulate air)
filter and ULPA (Ultra low penetration air

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10
Q

filter air coming inside OF BSC

A

GRILLS

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11
Q

Used to provide optimum temperature for
growtH

A

INCUBATOR

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12
Q

to circulate hot air to dry plates
paglabas kasi sa autoclave basa pa sample

HOT AIR OVEN

A

FAN

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13
Q

HOT AIR OVEN STERILIZING SETTING

A
  • Sterilizing setting 160-180c for 2 hrs
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14
Q
  • Oxidizes constituents of bacterial cell
A

HOT AIR OVEN

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15
Q

Used for stabbing culture and detecting
bacterial motility

A

INOCULATING LOOP OR NEEDLE

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16
Q

Need to be disinfecter then autoclaved before
throwing it out

A

INOCULATING LOOP OR NEEDLE

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17
Q
  • For mixing liquids
A

VORTEX MIXER/SHAKER

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18
Q

as cap-like depression in the top center
where the tubes are placed

A

VORTEX/MIXER

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19
Q

Moves in a dieratory motion affecting the
thorough mixing of what is placed inside the
test tube. speed can be adjusted

A

Vortex mixer/shaker

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20
Q
  • Melts media, solution, and sample
A

Water bath

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21
Q
  • Heats or melts sample and reagent
A

Heating mantle

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22
Q

For analyzing fluorescent material, especially
for spotting spots thin layer chromatography

A

UV VIEWING CABINET

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23
Q

Has a viewing port to avoid exposure to eyes
in the radiotion

A

UV VIEWING CABINET

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24
Q

No filter, confinement only though aerosols
and droplets might still leak outside

Study These Flashcards
A

INOCULATION CHAMBER

25
Used for testing pH for culture medium. If its too acidic, organisms wont grow h
PH METER
26
ALKALOPHILE EXAMPLE
CHOLERA
27
ACIDPHILE
enicyobactoprior
28
- Counting microbial colony
Colony counter
29
CFU
colony forming units
30
slows down the metabolism of bacteria
REF
31
Sterilization, heat fixation of smear, inoculation,transferring and plating culture, etc
BUNSEN BURNER
32
- Measuring sizes of bacterial cell
Stage ocular micrometer
33
- Can either be digital or triple beam balance
Weighing balance
34
3 TYPES OF THERMOMETER
1. Probe-type 2. Digital 3. Mercury
35
- Testing for coliform c water
MEMBRANE FILTER
36
- For sterilization liquids
MEMBRANE FILTER
37
Principle of microscopy
- Making small things visible
38
- Simplest microscope is
magnifying glass
39
HIGHEST MAGNIFICATION
1000X
40
TOTAL MAGNIFICATION
- Eyepiexe x objective lens
41
Ability of lens to distinguish 2 points at separate
Resolution
42
RESOLVING POWER
READ
43
DIameter of the circle of light that you see when working at a microscop
GIELD OF VIEW
44
MICROSCOPE THAT - Illuminates from light
BRIGHT FIELD
45
Has a special condenser diaphragm that occludes direct light
DARK-FIEKLD
46
Ring-shape condenser shifts light so theres an exchange of intensity in light (has contrast making you see better than in bright field)
PHASE CONTRAST
47
Best for analyzing urine and body fluids
PHASE COCNTRAST
48
- Uses electron beams and magnets
ELECTRON MICROSCOPE
49
More powerful because it has shorter waves of light
ELECTRON MICROSCOPE
50
Use chloroform light para maglow yung reagent
FLOURESCENT MICROSCOPE
51
- Uses uv or fluorescent light
FLOURESCEWNT MICROSCOPE
52
- Uses uv or fluorescent light
LENS AND OBJECTIVE
53
4 OBJECTIVES
● Scanning Objective Lens (4x) ● Low Power Lens (10x) ● High Power Lens (40x) ● Oil Immersion Lens (100x
54
LOBGEST OBJ
OIL IMMERSION
55
SHORTEST
SCANNER
56
- Focuses light
CONDENSER
57
- used so that there is no stray light
IMMERSION OIL
58
Buksan sa dimmer bg so that light going upwards can be regulated
Iris diaphragm
59

BACTE LAB (4 decks)

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