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ASCP Microbiology > Wordsology High Yield Notes - Gram Positive Cocci > Flashcards

Wordsology High Yield Notes - Gram Positive Cocci Flashcards

1
Q

What two organisms are coagulase positive?

A

Staph aureus and Staph intermedius

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2
Q

What two types of Gram positive cocci are catalase positive, coagulase negative?

A

Coagulase negative Staph and Micrococcus

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3
Q

What are characteristics of Gram positive, coagulase positive Staphylococcus?

A

Staph aureus and Staph intermedius

  • Salt tolerant
  • Beta hemolytic
  • Hyaluronidase
  • Enterotoxins –> scalded skin syndrome
  • Cause toxic shock syndrome (TSS)
  • 50% people have as normal flora
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4
Q

What test(s) differentiate coagulase negative Staphylococcus from Micrococcus?

A

Oxidase/Microdase (modified oxidase test)
and
Bacitracin/Lysostaphin

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5
Q

Are coagulase-negative Staphylococcus oxidase/microdase positive or negative?

A

Oxidase/Microdase negative

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6
Q

Are coagulase-negative Staphylococcus bacitracin resistant or susceptible?

A

Resistant

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7
Q

What is susceptible to and shows a >16mm zone of inhibition to Novobiocin?

A

Staphylococcus epidermidis

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8
Q

What organism is an opportunistic pathogen and may cause endocarditis if allowed the opportunity to enter surgical wounds?

A

Staph epidermidis

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9
Q

What organism possesses glycocalyx (capsule/slime layer/biofilm)?

A

Staph epidermidis

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10
Q

What Gram-positive organism causes UTI in sexually active females?

A

Staph saprophyticus

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11
Q

Novobiocin is used to differentiate:

A 
Staphylococcus epidermidis (susceptible) 
from 
Staphylococcus saprophyticus (resistant)
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12
Q

A gram positive organism is bacitracin susceptible and oxidase positive. What could it be?

A

Micrococcus

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13
Q

What test would you perform if you saw an alpha-hemolytic streptococcus colony?

A

Optochin disk to determine if it is Strep pneumo (susecptible).

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14
Q

What organism is lancet shaped in pairs?

A

Strep pneumoniae

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15
Q

What organism has glistening, wet, domed colonies?

A

Strep. pneumo

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16
Q

What organism has a quellung reaction (capsular swelling)?

A

Strep pneumoniae

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17
Q

What virulence factor does Strep pneumoniae have that protects it from phagocytosis?

A

Polysaccharide capsule

18
Q

What organism causes otitis media in children?

A

Strep. pneumoniae

19
Q

What is the optochin disk zone of inhibition for Strep pneumoniae?

A

> 14mm

20
Q

What does Strep. pneumoniae need in order to grow?

A

CO2

Brain heart infusion agar
TSA
Chocolate agar

21
Q

What type of organism can be found to be implicated in dental infections?

A

Strep. viridians

22
Q

What type of organism can cause subacute endocarditis?

A

Strep viridians

23
Q

What organism grows in low O2 and can be found in abscesses of the brain or abdomen?

A

Strep. anginosus, a type of viridians strep

24
Q

What type of Strep is Strep. anginosus?

A

Viridians Strep

25
Q

What environment can S. anginosus grow in and where is it usually found?

A

Low O2 environment

Found in abscesses of brain or abdomen

26
Q

Enterococcus is PRY ____?

A

Enterococcus is PRY positive.

27
Q

Group D Strep is PYR _____.

A

Group D Strep is PYR negative.

28
Q

Which one can tolerate salt: Enterococcus or Group D Strep?

A

Enterococcus - can grow in 6.5% NaCl

29
Q

What is the hemolytic pattern of enterococcus?

A

Enterococcus can be alpha or beta hemolytic.

30
Q

What test(s) differentiates Enterococcus from Group D Strep?

A

PRY and 6.5% NaCl

31
Q

What organism has Protein A as a virulence factor?

A

Staph aureus

32
Q

What is the first test you would perform if you wanted to ID a beta hemolytic Streptococcus?

A

PRY and Bacitracin

33
Q

Bacitracin and PYR result for Strep pyogenes:

A

Bacitracin susceptible, PYR positive

34
Q

Bacitracin and PYR result for Strep. agalactiae:

A

Bacitracin resistant and PYR negative

35
Q

What organism causes Scarlet Fever?

A

Strep. pyogenes (Group A Strep)

36
Q

What organism causes Strep throat?

A

S. pyogenes

37
Q

What Strep is the only one that is hippurate positive? What indicates a positive hippurate test?

A

Strep. agalactiae (Group B)

Purple color positive

38
Q

Is Strep. agalactiae bile positive or negative?

A

Positive

39
Q

What organism can cause neonatal sepsis-meningitis?

A

Group B Strep (can be transmitted to baby)

40
Q

Group B strep capsule contains what?

A

Sialic acid

41
Q

What organism’s capsule is anti-phagocytic and contains sialic acid?

A

S. agalactiae

ASCP Microbiology (10 decks)

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