Wordsology High Yield Notes - Gram Positive Cocci

Question 1

What two organisms are coagulase positive?

Answer 1

Staph aureus and Staph intermedius

Question 2

What two types of Gram positive cocci are catalase positive, coagulase negative?

Answer 2

Coagulase negative Staph and Micrococcus

Question 3

What are characteristics of Gram positive, coagulase positive Staphylococcus?

Answer 3

Staph aureus and Staph intermedius

• Salt tolerant
• Beta hemolytic
• Hyaluronidase
• Enterotoxins –> scalded skin syndrome
• Cause toxic shock syndrome (TSS)
• 50% people have as normal flora

Question 4

What test(s) differentiate coagulase negative Staphylococcus from Micrococcus?

Answer 4

Oxidase/Microdase (modified oxidase test)
and
Bacitracin/Lysostaphin

Question 5

Are coagulase-negative Staphylococcus oxidase/microdase positive or negative?

Answer 5

Oxidase/Microdase negative

Question 6

Are coagulase-negative Staphylococcus bacitracin resistant or susceptible?

Answer 6

Resistant

Question 7

What is susceptible to and shows a >16mm zone of inhibition to Novobiocin?

Answer 7

Staphylococcus epidermidis

Question 8

What organism is an opportunistic pathogen and may cause endocarditis if allowed the opportunity to enter surgical wounds?

Answer 8

Staph epidermidis

Question 9

What organism possesses glycocalyx (capsule/slime layer/biofilm)?

Answer 9

Staph epidermidis

Question 10

What Gram-positive organism causes UTI in sexually active females?

Answer 10

Staph saprophyticus

Question 11

Novobiocin is used to differentiate:

Answer 11

Staphylococcus epidermidis (susceptible) 
from 
Staphylococcus saprophyticus (resistant)

Question 12

A gram positive organism is bacitracin susceptible and oxidase positive. What could it be?

Answer 12

Micrococcus

Question 13

What test would you perform if you saw an alpha-hemolytic streptococcus colony?

Answer 13

Optochin disk to determine if it is Strep pneumo (susecptible).

Question 14

What organism is lancet shaped in pairs?

Answer 14

Strep pneumoniae

Question 15

What organism has glistening, wet, domed colonies?

Answer 15

Strep. pneumo

Question 16

What organism has a quellung reaction (capsular swelling)?

Answer 16

Strep pneumoniae

Question 17

What virulence factor does Strep pneumoniae have that protects it from phagocytosis?

Answer 17

Polysaccharide capsule

Question 18

What organism causes otitis media in children?

Answer 18

Strep. pneumoniae

Question 19

What is the optochin disk zone of inhibition for Strep pneumoniae?

Answer 19

> 14mm

Question 20

What does Strep. pneumoniae need in order to grow?

Answer 20

CO2

Brain heart infusion agar
TSA
Chocolate agar

Question 21

What type of organism can be found to be implicated in dental infections?

Answer 21

Strep. viridians

Question 22

What type of organism can cause subacute endocarditis?

Answer 22

Strep viridians

Question 23

What organism grows in low O2 and can be found in abscesses of the brain or abdomen?

Answer 23

Strep. anginosus, a type of viridians strep

Question 24

What type of Strep is Strep. anginosus?

Answer 24

Viridians Strep

Question 25

What environment can S. anginosus grow in and where is it usually found?

Answer 25

Low O2 environment | Found in abscesses of brain or abdomen

Question 26

Enterococcus is PRY ____?

Answer 26

Enterococcus is PRY positive.

Question 27

Group D Strep is PYR _____.

Answer 27

Group D Strep is PYR negative.

Question 28

Which one can tolerate salt: Enterococcus or Group D Strep?

Answer 28

Enterococcus - can grow in 6.5% NaCl

Question 29

What is the hemolytic pattern of enterococcus?

Answer 29

Enterococcus can be alpha or beta hemolytic.

Question 30

What test(s) differentiates Enterococcus from Group D Strep?

Answer 30

PRY and 6.5% NaCl

Question 31

What organism has Protein A as a virulence factor?

Answer 31

Staph aureus

Question 32

What is the first test you would perform if you wanted to ID a beta hemolytic Streptococcus?

Answer 32

PRY and Bacitracin

Question 33

Bacitracin and PYR result for Strep pyogenes:

Answer 33

Bacitracin susceptible, PYR positive

Question 34

Bacitracin and PYR result for Strep. agalactiae:

Answer 34

Bacitracin resistant and PYR negative

Question 35

What organism causes Scarlet Fever?

Answer 35

Strep. pyogenes (Group A Strep)

Question 36

What organism causes Strep throat?

Answer 36

S. pyogenes

Question 37

What Strep is the only one that is hippurate positive? What indicates a positive hippurate test?

Answer 37

Strep. agalactiae (Group B) Purple color positive

Question 38

Is Strep. agalactiae bile positive or negative?

Answer 38

Positive

Question 39

What organism can cause neonatal sepsis-meningitis?

Answer 39

Group B Strep (can be transmitted to baby)

Question 40

Group B strep capsule contains what?

Answer 40

Sialic acid

Question 41

What organism's capsule is anti-phagocytic and contains sialic acid?

Answer 41

S. agalactiae