Year 1 Flashcards
(24 cards)
What are some aseptic techniques?
- Wash hands with soap oR
Disinfect surfaces: - Use sterile pipette/syringe (to transfer bacteria);
- (Remove bottle lid and) flame neck of bottle;
- Lift lid of (agar) plate at an
angle; - Work close to upward air movement;
- Use sterile spreader;
- Place pipette/spreader into disinfectant immediately after use);
why do we add hydrochloric acid to root tip?
to soften and loosen the root tissues for when they squished under slide for micrscope
why do we use a dye for the root tip
to stain the chromsomes
why do we use mounted needle to
ensure no bubble under the slip so clear image in micrscope
why do we use last 5mm of the root tip
this is the growing region of cells so this is where mitosis would be occuring
why do we have to make sure we press down on the root tip without sliding it
thin layer of cells to allow light to pass through
and ensure there are no overlapping cells
risk assesment of scalpel
cut on top of a white tile to prevent slipping (hard surface)
- cut away from body
risk of hydrochloric acid
wear goggles and wash ahnds if any spills
what does not being able to see visible chromosomes in root tip squash?
cell is not in mitosis
whatgs miotic index
miotic index= number of cells in mitosis/ total number of cells x100
how to ensure miotic undex count is accurate?
examine large number of fields of view
- to ensure representative sample
why do we control the temp in potato osmosis practical
temp affects the rate of kinetic energy
- so would affect te rate of osmosims , omsosis would occur at a faster rate
why do we blot potato discs dry
to ensure any access water is removed and to get an accurate mass reading
alcohol and beetroot
alcohol damages the cell membranes of betroot cells = making pigment leak out of cells
- does this by denaturing protein channels found on phospholipid bilayer
what does a colorimeter metre do
turn qualitative observations of colour into quantitative values which are absorbance of light
to then use the data to plot a calibration curve
why do we blot betroot dry?
to remove pigment that has been released from breaking of cells when cutting the betroot into discs
why would high temp result in pigment being released in betroot cells
- cause membrane proteins to denature
- damaging the phospholipid bilayer and breaking open cells
- more pigment is released
why would ethanol/ alchohol result in pigment being released in betroot cells
- lipids are soluble in alchocol so the alchohol will start to dissolbe the phoshpolipid bilayer
- damaaging cells
-causing pigment to be released
why would acid result in pigment being released in betroot cells
- acid denatures membrane protein
- damaging cell membrane
- releasing pigment
heart disection risk assessment
- wear lab coat
- wear gloves
- disinfect surface
- disnifect equipment
how to do a scientific drawing
- no shading
- no arrows
- have to have a title
- have a scale
- no cross hatching
- no overlapping lines
why must u keep the bunsen burner on during aseptic technique
to kill bacteria found in the air
why must u make sure to not tape the whole lid around in an agar plate?
to allow 02 to enter for respiration
what is the inhibition zone in aseptic technique
where bacteria didnt grow so this shows that the antiobitoic was able to kill the bacteria
